ENSG00000165526

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000298317 ENSG00000165526 HEK293_OSMI2_2hA HEK293_TMG_2hB RPUSD4 protein_coding protein_coding 32.50073 36.49178 30.42229 4.011245 0.5188082 -0.2623639 16.090444 21.9941865 11.190958 3.3197628 0.69510417 -0.9741559 0.49138333 0.59696667 0.3679000 -0.22906667 2.161155e-05 2.098344e-09 FALSE TRUE
ENST00000526942 ENSG00000165526 HEK293_OSMI2_2hA HEK293_TMG_2hB RPUSD4 protein_coding retained_intron 32.50073 36.49178 30.42229 4.011245 0.5188082 -0.2623639 1.950710 0.6637424 4.494488 0.3766732 0.27728137 2.7410942 0.06406250 0.02106667 0.1479000 0.12683333 1.445323e-01 2.098344e-09 FALSE TRUE
ENST00000530903 ENSG00000165526 HEK293_OSMI2_2hA HEK293_TMG_2hB RPUSD4 protein_coding retained_intron 32.50073 36.49178 30.42229 4.011245 0.5188082 -0.2623639 2.851658 1.6931671 3.365068 0.1089334 0.06380455 0.9866965 0.08937083 0.04690000 0.1106333 0.06373333 2.098344e-09 2.098344e-09 FALSE TRUE
ENST00000532800 ENSG00000165526 HEK293_OSMI2_2hA HEK293_TMG_2hB RPUSD4 protein_coding retained_intron 32.50073 36.49178 30.42229 4.011245 0.5188082 -0.2623639 1.952884 0.4831591 3.291487 0.1181474 0.21076692 2.7429908 0.06205000 0.01413333 0.1084000 0.09426667 2.442038e-08 2.098344e-09 FALSE TRUE
ENST00000533628 ENSG00000165526 HEK293_OSMI2_2hA HEK293_TMG_2hB RPUSD4 protein_coding protein_coding 32.50073 36.49178 30.42229 4.011245 0.5188082 -0.2623639 7.987400 10.4662640 5.932966 0.9310390 0.57220743 -0.8178693 0.24198333 0.28876667 0.1956667 -0.09310000 5.485910e-02 2.098344e-09 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000165526 E001 52.7310876 0.0031859013 1.930807e-02 5.000214e-02 11 126202096 126202413 318 - 1.620 1.760 0.475
ENSG00000165526 E002 339.0215232 0.0001486429 3.414497e-22 2.772680e-20 11 126202414 126202781 368 - 2.369 2.573 0.679
ENSG00000165526 E003 471.9156921 0.0010738164 1.235276e-07 1.232783e-06 11 126202782 126203061 280 - 2.575 2.688 0.375
ENSG00000165526 E004 406.6848791 0.0001292073 1.402263e-04 7.045791e-04 11 126203062 126203321 260 - 2.544 2.615 0.234
ENSG00000165526 E005 429.6709090 0.0002356517 8.787030e-01 9.260911e-01 11 126203322 126203654 333 - 2.613 2.616 0.009
ENSG00000165526 E006 103.8307877 0.0003787992 1.547275e-01 2.680612e-01 11 126203655 126203657 3 - 2.035 1.985 -0.167
ENSG00000165526 E007 184.2685230 0.0002558734 5.015513e-01 6.376920e-01 11 126204231 126204324 94 - 2.260 2.243 -0.058
ENSG00000165526 E008 91.5650646 0.0003441365 6.330537e-01 7.478178e-01 11 126204325 126204328 4 - 1.933 1.952 0.064
ENSG00000165526 E009 149.2084691 0.0002380472 9.723975e-01 9.867788e-01 11 126205468 126205519 52 - 2.156 2.158 0.007
ENSG00000165526 E010 114.7278391 0.0002626184 3.371063e-01 4.809635e-01 11 126205520 126205539 20 - 2.066 2.034 -0.105
ENSG00000165526 E011 118.2718290 0.0002719897 1.408605e-01 2.492072e-01 11 126205540 126205570 31 - 2.089 2.040 -0.161
ENSG00000165526 E012 149.8869916 0.0002477463 6.407014e-01 7.539066e-01 11 126205571 126205612 42 - 2.168 2.155 -0.043
ENSG00000165526 E013 133.6437892 0.0002513499 7.687689e-01 8.505624e-01 11 126205688 126205707 20 - 2.105 2.115 0.036
ENSG00000165526 E014 138.6384456 0.0002788866 9.850661e-01 9.946850e-01 11 126205708 126205718 11 - 2.127 2.128 0.002
ENSG00000165526 E015 184.4841341 0.0015617750 4.272715e-01 5.701016e-01 11 126205719 126205781 63 - 2.266 2.241 -0.085
ENSG00000165526 E016 43.3452797 0.0007419339 8.910903e-14 2.551994e-12 11 126205782 126206253 472 - 1.846 1.451 -1.344
ENSG00000165526 E017 32.8973676 0.0086639304 6.674768e-14 1.943613e-12 11 126206254 126206553 300 - 1.797 1.232 -1.940
ENSG00000165526 E018 20.2686818 0.0020781948 3.480016e-26 4.209953e-24 11 126209040 126209490 451 - 1.677 0.800 -3.133
ENSG00000165526 E019 7.2553358 0.0022136025 1.345066e-12 3.228824e-11 11 126209491 126209493 3 - 1.270 0.401 -3.539
ENSG00000165526 E020 13.2510010 0.0016768947 1.552092e-13 4.292543e-12 11 126209494 126209520 27 - 1.458 0.763 -2.528
ENSG00000165526 E021 153.6878380 0.0006472715 5.709768e-01 6.973796e-01 11 126209521 126209550 30 - 2.184 2.168 -0.053
ENSG00000165526 E022 186.9972113 0.0015299017 5.501692e-01 6.798285e-01 11 126209551 126209605 55 - 2.268 2.249 -0.063
ENSG00000165526 E023 247.7178293 0.0014677468 6.635312e-02 1.373119e-01 11 126209606 126209722 117 - 2.411 2.356 -0.183
ENSG00000165526 E024 0.6558305 0.0994402160 6.390415e-01 7.525236e-01 11 126209723 126209723 1 - 0.264 0.178 -0.727
ENSG00000165526 E025 258.5204543 0.0002164417 9.736406e-03 2.811458e-02 11 126210890 126211055 166 - 2.434 2.377 -0.190
ENSG00000165526 E026 1.5885526 0.0094972315 6.308876e-01 7.460147e-01 11 126211056 126211297 242 - 0.354 0.442 0.488
ENSG00000165526 E027 1.7819870 0.0102945140 2.604832e-01 3.980130e-01 11 126211333 126211449 117 - 0.546 0.355 -0.995
ENSG00000165526 E028 173.0404869 0.0002269662 4.470000e-04 1.955452e-03 11 126211450 126211692 243 - 2.285 2.191 -0.315