ENSG00000165525

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000298310 ENSG00000165525 HEK293_OSMI2_2hA HEK293_TMG_2hB NEMF protein_coding protein_coding 12.6041 2.878429 20.43054 0.2984752 0.9321734 2.823076 0.918699 0.435070 1.354860 0.1103345 0.1844913 1.616649 0.09096667 0.1467 0.06666667 -0.08003333 3.626304e-02 1.201397e-18 FALSE TRUE
ENST00000555970 ENSG00000165525 HEK293_OSMI2_2hA HEK293_TMG_2hB NEMF protein_coding protein_coding 12.6041 2.878429 20.43054 0.2984752 0.9321734 2.823076 3.397977 0.000000 7.125659 0.0000000 0.8977766 9.478903 0.17827917 0.0000 0.34643333 0.34643333 1.201397e-18 1.201397e-18 FALSE TRUE
MSTRG.9495.2 ENSG00000165525 HEK293_OSMI2_2hA HEK293_TMG_2hB NEMF protein_coding   12.6041 2.878429 20.43054 0.2984752 0.9321734 2.823076 6.375639 1.941218 9.409139 0.1583077 0.5678820 2.271220 0.56234583 0.6778 0.46310000 -0.21470000 1.036849e-02 1.201397e-18 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000165525 E001 34.5117568 0.0047266395 2.493436e-05 1.512419e-04 14 49782083 49783813 1731 - 1.341 1.676 1.149
ENSG00000165525 E002 2.1799423 0.0065432883 4.772459e-01 6.158323e-01 14 49783814 49783814 1 - 0.387 0.536 0.759
ENSG00000165525 E003 3.4687881 0.0044597129 3.934390e-02 8.980980e-02 14 49783815 49783827 13 - 0.471 0.824 1.533
ENSG00000165525 E004 130.1095917 0.0123381196 3.770595e-07 3.416693e-06 14 49783828 49784655 828 - 1.912 2.245 1.117
ENSG00000165525 E005 57.1200717 0.0015014667 9.652609e-04 3.839298e-03 14 49784656 49784713 58 - 1.591 1.802 0.715
ENSG00000165525 E006 1.1835302 0.0869683414 9.244965e-01 9.564336e-01 14 49784905 49784924 20 - 0.282 0.257 -0.182
ENSG00000165525 E007 45.0216604 0.0070097508 4.954630e-02 1.083567e-01 14 49784925 49784955 31 - 1.503 1.667 0.557
ENSG00000165525 E008 46.2441207 0.0143610125 2.721847e-02 6.643465e-02 14 49784956 49785004 49 - 1.504 1.705 0.687
ENSG00000165525 E009 4.0127328 0.0243478797 2.596076e-01 3.970282e-01 14 49785005 49785091 87 - 0.558 0.768 0.896
ENSG00000165525 E010 52.2496550 0.0007588829 9.493547e-04 3.785569e-03 14 49785092 49785135 44 - 1.553 1.768 0.727
ENSG00000165525 E011 4.3222150 0.0038425287 1.214895e-04 6.206736e-04 14 49785136 49785219 84 - 0.452 1.031 2.411
ENSG00000165525 E012 73.8608379 0.0003645768 8.723485e-05 4.620308e-04 14 49785220 49785320 101 - 1.701 1.914 0.718
ENSG00000165525 E013 4.5271929 0.0089071021 1.729950e-01 2.920850e-01 14 49785321 49785774 454 - 0.588 0.824 0.980
ENSG00000165525 E014 54.8491905 0.0007958467 2.426454e-03 8.541464e-03 14 49786718 49786750 33 - 1.578 1.774 0.664
ENSG00000165525 E015 2.9623847 0.0052585976 8.587229e-01 9.128644e-01 14 49786751 49787174 424 - 0.490 0.536 0.218
ENSG00000165525 E016 91.7943700 0.0005217536 2.522321e-02 6.233479e-02 14 49789146 49789343 198 - 1.813 1.935 0.411
ENSG00000165525 E017 44.7186346 0.0200575352 5.179159e-01 6.522346e-01 14 49789496 49789573 78 - 1.538 1.485 -0.183
ENSG00000165525 E018 80.0605115 0.0091190032 6.726031e-01 7.785197e-01 14 49795791 49795944 154 - 1.779 1.765 -0.049
ENSG00000165525 E019 0.5149111 0.0201126368 3.815271e-02 8.764192e-02 14 49795945 49796232 288 - 0.053 0.419 3.633
ENSG00000165525 E020 1.7984463 0.0109237732 1.456071e-02 3.949944e-02 14 49796233 49796328 96 - 0.251 0.704 2.371
ENSG00000165525 E021 0.6727692 0.0576794983 9.829519e-02 1.879291e-01 14 49797373 49797465 93 - 0.101 0.419 2.633
ENSG00000165525 E022 66.5542108 0.0003840251 3.345473e-01 4.782913e-01 14 49799475 49799524 50 - 1.685 1.755 0.238
ENSG00000165525 E023 63.1130084 0.0004291130 4.044648e-01 5.481725e-01 14 49799636 49799678 43 - 1.666 1.730 0.217
ENSG00000165525 E024 140.5779670 0.0022179500 2.888411e-01 4.294803e-01 14 49800420 49800650 231 - 2.010 2.071 0.206
ENSG00000165525 E025 80.3232442 0.0008371977 6.518186e-01 7.626208e-01 14 49800651 49800696 46 - 1.774 1.813 0.133
ENSG00000165525 E026 1.3598103 0.0858772700 8.065541e-01 8.770678e-01 14 49800697 49801519 823 - 0.311 0.258 -0.364
ENSG00000165525 E027 103.5359402 0.0003105891 3.507315e-01 4.949167e-01 14 49802453 49802573 121 - 1.893 1.864 -0.098
ENSG00000165525 E028 56.1563078 0.0003925819 3.098216e-01 4.521625e-01 14 49802669 49802727 59 - 1.634 1.583 -0.174
ENSG00000165525 E029 69.2093665 0.0003542773 1.583149e-01 2.728878e-01 14 49803237 49803294 58 - 1.728 1.659 -0.233
ENSG00000165525 E030 73.5412153 0.0018802980 2.398643e-01 3.739831e-01 14 49806021 49806133 113 - 1.751 1.696 -0.188
ENSG00000165525 E031 47.3031987 0.0006454550 6.435965e-01 7.560969e-01 14 49813988 49814050 63 - 1.545 1.592 0.160
ENSG00000165525 E032 40.7552246 0.0007946719 1.660767e-01 2.831011e-01 14 49814754 49814807 54 - 1.470 1.583 0.389
ENSG00000165525 E033 37.3039971 0.0012939954 2.609296e-01 3.985281e-01 14 49814808 49814857 50 - 1.466 1.389 -0.263
ENSG00000165525 E034 1.0478088 0.1901982736 3.740413e-01 5.184560e-01 14 49818293 49818421 129 - 0.283 0.000 -10.427
ENSG00000165525 E035 3.3495129 0.0377040493 1.178311e-01 2.168331e-01 14 49820096 49820456 361 - 0.471 0.770 1.318
ENSG00000165525 E036 55.6491687 0.0004436446 1.524984e-02 4.105144e-02 14 49825867 49825955 89 - 1.645 1.491 -0.524
ENSG00000165525 E037 33.1773560 0.0007712268 6.368866e-02 1.328433e-01 14 49828291 49828291 1 - 1.425 1.275 -0.522
ENSG00000165525 E038 53.6117310 0.0004572711 2.102761e-02 5.364740e-02 14 49828292 49828354 63 - 1.628 1.480 -0.505
ENSG00000165525 E039 0.0000000       14 49828613 49828615 3 -      
ENSG00000165525 E040 81.6487149 0.0004395751 6.824639e-05 3.716960e-04 14 49828616 49828787 172 - 1.817 1.592 -0.761
ENSG00000165525 E041 33.1182904 0.0021357343 1.009996e-04 5.265721e-04 14 49828788 49828807 20 - 1.450 1.062 -1.367
ENSG00000165525 E042 91.2802763 0.0012832472 3.071855e-02 7.336415e-02 14 49829054 49829262 209 - 1.847 1.744 -0.351
ENSG00000165525 E043 0.4482035 0.0262950599 1.000000e+00 1.000000e+00 14 49829263 49829348 86 - 0.144 0.000 -9.199
ENSG00000165525 E044 58.8353284 0.0004754962 3.097833e-01 4.521241e-01 14 49829349 49829426 78 - 1.651 1.601 -0.169
ENSG00000165525 E045 53.0583071 0.0004384873 1.652795e-02 4.390313e-02 14 49831299 49831361 63 - 1.624 1.468 -0.532
ENSG00000165525 E046 49.2315104 0.0005047741 2.489323e-01 3.846770e-01 14 49832051 49832126 76 - 1.580 1.514 -0.226
ENSG00000165525 E047 52.0865294 0.0007148076 1.208016e-01 2.210968e-01 14 49832207 49832277 71 - 1.607 1.514 -0.319
ENSG00000165525 E048 43.5484635 0.0005771732 5.511371e-02 1.181941e-01 14 49833423 49833496 74 - 1.537 1.404 -0.460
ENSG00000165525 E049 57.6561889 0.0004025970 4.418563e-03 1.431548e-02 14 49834363 49834449 87 - 1.662 1.480 -0.623
ENSG00000165525 E050 44.7467970 0.0005096201 6.882881e-03 2.092030e-02 14 49838139 49838206 68 - 1.558 1.360 -0.684
ENSG00000165525 E051 60.0466811 0.0033035607 4.740436e-03 1.520669e-02 14 49840718 49840866 149 - 1.683 1.491 -0.653
ENSG00000165525 E052 1.6126024 0.0112293928 4.943538e-02 1.081566e-01 14 49845975 49846138 164 - 0.251 0.628 2.049
ENSG00000165525 E053 1.5760707 0.0109630727 2.085266e-01 3.367091e-01 14 49846139 49846139 1 - 0.282 0.536 1.411
ENSG00000165525 E054 56.9069703 0.0004550429 6.371709e-03 1.958912e-02 14 49846140 49846265 126 - 1.655 1.480 -0.598
ENSG00000165525 E055 0.0000000       14 49849629 49849707 79 -      
ENSG00000165525 E056 64.0793277 0.0004063305 8.701543e-04 3.509871e-03 14 49851563 49851665 103 - 1.710 1.503 -0.708
ENSG00000165525 E057 53.6060723 0.0004697370 9.300748e-05 4.891588e-04 14 49851807 49851875 69 - 1.642 1.360 -0.970
ENSG00000165525 E058 49.2119642 0.0006531827 1.688492e-03 6.246186e-03 14 49852695 49852945 251 - 1.601 1.375 -0.779