ENSG00000165521

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000553526 ENSG00000165521 HEK293_OSMI2_2hA HEK293_TMG_2hB EML5 protein_coding nonsense_mediated_decay 1.850398 0.7162321 2.184536 0.03415663 0.044713 1.595413 0.3482140 0.2648075 0.0000000 0.03246149 0.00000000 -4.780349 0.23541250 0.3718 0.0000 -0.3718 1.018496e-10 1.018496e-10 FALSE TRUE
ENST00000554922 ENSG00000165521 HEK293_OSMI2_2hA HEK293_TMG_2hB EML5 protein_coding protein_coding 1.850398 0.7162321 2.184536 0.03415663 0.044713 1.595413 0.8324241 0.4198193 0.9590351 0.06838994 0.03059323 1.172819 0.49343750 0.5813 0.4399 -0.1414 4.702739e-01 1.018496e-10 FALSE TRUE
MSTRG.10044.2 ENSG00000165521 HEK293_OSMI2_2hA HEK293_TMG_2hB EML5 protein_coding   1.850398 0.7162321 2.184536 0.03415663 0.044713 1.595413 0.2033729 0.0000000 0.3529089 0.00000000 0.18836336 5.181536 0.07580417 0.0000 0.1605 0.1605 3.746722e-01 1.018496e-10 FALSE TRUE
MSTRG.10044.4 ENSG00000165521 HEK293_OSMI2_2hA HEK293_TMG_2hB EML5 protein_coding   1.850398 0.7162321 2.184536 0.03415663 0.044713 1.595413 0.1946680 0.0000000 0.4976551 0.00000000 0.25069293 5.665777 0.07397500 0.0000 0.2317 0.2317 3.546559e-01 1.018496e-10 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000165521 E001 32.1723026 0.0007743275 1.395718e-06 1.122779e-05 14 88612431 88613720 1290 - 1.363 1.668 1.046
ENSG00000165521 E002 26.5069669 0.0008768324 2.819797e-01 4.219439e-01 14 88613721 88614870 1150 - 1.366 1.449 0.285
ENSG00000165521 E003 37.8242675 0.0128911449 1.835767e-02 4.791686e-02 14 88614871 88615817 947 - 1.486 1.671 0.631
ENSG00000165521 E004 6.6868724 0.0025216312 3.869602e-01 5.311168e-01 14 88615818 88615854 37 - 0.814 0.929 0.442
ENSG00000165521 E005 11.0176969 0.0017853069 2.554074e-01 3.922926e-01 14 88616142 88616242 101 - 0.992 1.118 0.457
ENSG00000165521 E006 11.9154232 0.1365307626 9.005297e-01 9.405451e-01 14 88616726 88616879 154 - 1.032 1.122 0.328
ENSG00000165521 E007 10.6547151 0.1207630373 6.290601e-01 7.445094e-01 14 88618228 88618331 104 - 0.976 1.098 0.447
ENSG00000165521 E008 0.0000000       14 88618648 88618649 2 -      
ENSG00000165521 E009 7.9718642 0.0177515360 6.593622e-02 1.365929e-01 14 88618650 88618722 73 - 0.814 1.059 0.925
ENSG00000165521 E010 8.3108590 0.0469697146 1.831717e-02 4.782699e-02 14 88618723 88618812 90 - 0.801 1.139 1.263
ENSG00000165521 E011 0.0000000       14 88618813 88619712 900 -      
ENSG00000165521 E012 11.1311967 0.0017986873 4.678771e-01 6.073269e-01 14 88620754 88620923 170 - 1.016 1.098 0.299
ENSG00000165521 E013 4.8610686 0.0034017529 9.488668e-02 1.827108e-01 14 88620924 88620926 3 - 0.789 0.519 -1.158
ENSG00000165521 E014 11.6962223 0.0015862983 4.925269e-01 6.296934e-01 14 88621113 88621209 97 - 1.080 1.009 -0.258
ENSG00000165521 E015 11.1255714 0.0028239738 5.167238e-01 6.512572e-01 14 88621210 88621301 92 - 1.023 1.098 0.270
ENSG00000165521 E016 0.8449086 0.0138339990 9.570765e-03 2.770964e-02 14 88621757 88621904 148 - 0.073 0.519 3.647
ENSG00000165521 E017 12.6600729 0.0041631826 6.806091e-02 1.401482e-01 14 88622604 88622718 115 - 1.031 1.219 0.676
ENSG00000165521 E018 11.4974734 0.0021676121 3.972582e-01 5.410248e-01 14 88624970 88625127 158 - 1.023 1.117 0.341
ENSG00000165521 E019 13.8820122 0.0063579849 9.392324e-01 9.658359e-01 14 88626838 88627046 209 - 1.124 1.136 0.044
ENSG00000165521 E020 10.4354600 0.0080041444 7.658723e-01 8.485420e-01 14 88627646 88627747 102 - 1.016 0.985 -0.112
ENSG00000165521 E021 8.8359585 0.0782943828 7.899858e-01 8.656547e-01 14 88627748 88627819 72 - 0.940 1.001 0.228
ENSG00000165521 E022 7.0072069 0.0652630916 6.987077e-01 7.986111e-01 14 88634469 88634489 21 - 0.849 0.922 0.280
ENSG00000165521 E023 9.1316010 0.0453047724 7.634281e-01 8.467304e-01 14 88638809 88638907 99 - 0.949 1.008 0.218
ENSG00000165521 E024 9.2059004 0.0032146622 8.467656e-01 9.047783e-01 14 88642893 88643022 130 - 0.976 0.957 -0.068
ENSG00000165521 E025 8.0351563 0.0021978784 9.203176e-01 9.536676e-01 14 88644433 88644511 79 - 0.912 0.929 0.064
ENSG00000165521 E026 0.0000000       14 88644512 88644737 226 -      
ENSG00000165521 E027 0.0000000       14 88646947 88646955 9 -      
ENSG00000165521 E028 0.0000000       14 88649912 88649926 15 -      
ENSG00000165521 E029 11.4462251 0.0032742645 2.676753e-01 4.060071e-01 14 88657376 88657502 127 - 1.080 0.958 -0.451
ENSG00000165521 E030 12.5608551 0.0050549083 3.378014e-01 4.816868e-01 14 88658187 88658388 202 - 1.112 1.010 -0.373
ENSG00000165521 E031 9.7458161 0.0143862329 5.572288e-01 6.856890e-01 14 88661654 88661830 177 - 0.959 1.035 0.281
ENSG00000165521 E032 6.5731495 0.0026568402 7.905341e-01 8.660101e-01 14 88663031 88663114 84 - 0.826 0.866 0.154
ENSG00000165521 E033 3.6484067 0.0047529754 1.480152e-01 2.589725e-01 14 88663115 88663119 5 - 0.687 0.436 -1.158
ENSG00000165521 E034 8.7007396 0.0020407853 8.962975e-01 9.377205e-01 14 88664493 88664624 132 - 0.940 0.929 -0.042
ENSG00000165521 E035 11.0173072 0.0021554718 9.622207e-01 9.801741e-01 14 88665337 88665489 153 - 1.023 1.034 0.038
ENSG00000165521 E036 12.5362499 0.0014994535 3.508690e-01 4.950517e-01 14 88681890 88682031 142 - 1.106 1.010 -0.350
ENSG00000165521 E037 11.3765834 0.0235965215 2.170036e-01 3.467909e-01 14 88685015 88685142 128 - 1.093 0.928 -0.607
ENSG00000165521 E038 10.2034468 0.0042811776 5.082180e-01 6.436804e-01 14 88687216 88687327 112 - 1.031 0.957 -0.272
ENSG00000165521 E039 9.2991909 0.0019300364 2.902041e-02 7.004765e-02 14 88688271 88688359 89 - 1.031 0.749 -1.079
ENSG00000165521 E040 9.0009401 0.0202889671 9.757847e-01 9.889077e-01 14 88688360 88688473 114 - 0.950 0.959 0.034
ENSG00000165521 E041 10.6197740 0.0313946878 6.937979e-01 7.947872e-01 14 88694307 88694407 101 - 1.032 0.986 -0.168
ENSG00000165521 E042 8.6678391 0.0548819155 6.150436e-02 1.291675e-01 14 88695361 88695454 94 - 1.009 0.702 -1.189
ENSG00000165521 E043 6.3142196 0.0026576736 3.425115e-03 1.151167e-02 14 88696847 88696952 106 - 0.912 0.436 -2.051
ENSG00000165521 E044 8.9964617 0.0050604949 2.145772e-02 5.455487e-02 14 88702446 88702632 187 - 1.016 0.703 -1.213
ENSG00000165521 E045 10.6453762 0.0136443687 6.234317e-01 7.400203e-01 14 88704860 88704978 119 - 0.992 1.059 0.246
ENSG00000165521 E046 10.7221412 0.0128549090 4.523143e-01 5.931717e-01 14 88705482 88705588 107 - 0.984 1.080 0.353
ENSG00000165521 E047 0.0000000       14 88705720 88705873 154 -      
ENSG00000165521 E048 7.8700280 0.0441484360 2.438735e-01 3.787442e-01 14 88706259 88706426 168 - 0.941 0.754 -0.728
ENSG00000165521 E049 9.0422702 0.0019766341 7.555016e-02 1.523946e-01 14 88712271 88712483 213 - 1.016 0.791 -0.854
ENSG00000165521 E050 12.3793236 0.0017368022 3.526237e-01 4.968762e-01 14 88714939 88715195 257 - 1.106 1.010 -0.350
ENSG00000165521 E051 8.6801276 0.0020826766 8.972345e-01 9.383719e-01 14 88726541 88726678 138 - 0.940 0.929 -0.042
ENSG00000165521 E052 12.0437175 0.0239766224 9.323947e-02 1.801873e-01 14 88736364 88736565 202 - 1.118 0.901 -0.803
ENSG00000165521 E053 7.7632405 0.1151312594 1.683502e-01 2.860474e-01 14 88738879 88739014 136 - 0.959 0.702 -1.004
ENSG00000165521 E054 9.6558085 0.0017597730 2.082987e-03 7.481008e-03 14 88740387 88740572 186 - 1.067 0.649 -1.624
ENSG00000165521 E055 6.8195657 0.0023990824 6.677763e-02 1.380311e-01 14 88744023 88744091 69 - 0.912 0.649 -1.051
ENSG00000165521 E056 9.9138741 0.0017084381 1.711601e-03 6.318473e-03 14 88746185 88746283 99 - 1.073 0.649 -1.648
ENSG00000165521 E057 9.2336267 0.0018372245 3.394600e-02 7.964836e-02 14 88754512 88754671 160 - 1.023 0.749 -1.051
ENSG00000165521 E058 10.0472110 0.0016930931 8.586311e-01 9.127925e-01 14 88792307 88792953 647 - 1.000 0.984 -0.058