ENSG00000165417

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000553612 ENSG00000165417 HEK293_OSMI2_2hA HEK293_TMG_2hB GTF2A1 protein_coding protein_coding 7.820912 2.683251 13.29882 0.5492479 0.4649548 2.304962 6.7201322 2.65449 11.5366331 0.5389372 0.3719463 2.115541 0.91180417 0.9898667 0.86773333 -0.12213333 3.535174e-07 3.535174e-07 FALSE TRUE
MSTRG.10032.2 ENSG00000165417 HEK293_OSMI2_2hA HEK293_TMG_2hB GTF2A1 protein_coding   7.820912 2.683251 13.29882 0.5492479 0.4649548 2.304962 0.5099876 0.00000 0.7383662 0.0000000 0.1479852 6.225672 0.03829583 0.0000000 0.05646667 0.05646667 9.366663e-04 3.535174e-07 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000165417 E001 758.699971 0.0020752408 8.804335e-43 3.579302e-40 14 81175452 81179660 4209 - 2.709 2.985 0.918
ENSG00000165417 E002 113.867894 0.0003021162 6.873324e-09 8.734442e-08 14 81179661 81180177 517 - 1.982 1.818 -0.550
ENSG00000165417 E003 22.764182 0.0008474229 2.003335e-01 3.266424e-01 14 81180178 81180208 31 - 1.283 1.258 -0.086
ENSG00000165417 E004 51.635124 0.0004546286 3.168353e-04 1.447954e-03 14 81180209 81180330 122 - 1.641 1.499 -0.486
ENSG00000165417 E005 65.163639 0.0003906270 2.061345e-03 7.415510e-03 14 81185531 81185620 90 - 1.732 1.653 -0.270
ENSG00000165417 E006 122.553699 0.0045374786 2.328803e-05 1.422076e-04 14 81192519 81192839 321 - 2.009 1.869 -0.472
ENSG00000165417 E007 59.255286 0.0061501490 1.046480e-03 4.119961e-03 14 81196108 81196241 134 - 1.700 1.547 -0.521
ENSG00000165417 E008 0.000000       14 81197227 81197408 182 -      
ENSG00000165417 E009 38.554656 0.0005648843 1.379187e-03 5.238755e-03 14 81197409 81197484 76 - 1.518 1.368 -0.516
ENSG00000165417 E010 39.184722 0.0010173083 1.214733e-04 6.206074e-04 14 81201594 81201658 65 - 1.533 1.325 -0.719
ENSG00000165417 E011 69.636626 0.0006544716 1.795685e-10 3.033052e-09 14 81203900 81204104 205 - 1.789 1.488 -1.023
ENSG00000165417 E012 1.497056 0.0991370841 4.847647e-01 6.227181e-01 14 81209870 81209936 67 - 0.358 0.241 -0.792
ENSG00000165417 E013 53.842674 0.0017711573 8.097922e-08 8.373141e-07 14 81216413 81216514 102 - 1.678 1.382 -1.014
ENSG00000165417 E014 57.901753 0.0031852040 2.516546e-07 2.366016e-06 14 81220489 81220959 471 - 1.706 1.420 -0.977
ENSG00000165417 E015 1.916655 0.0071390525 3.871641e-02 8.865732e-02 14 81221144 81221147 4 - 0.454 0.001 -10.502
ENSG00000165417 E016 5.516337 0.0029071768 1.521183e-02 4.096893e-02 14 81221148 81221699 552 - 0.776 0.396 -1.739