ENSG00000165406

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000319836 ENSG00000165406 HEK293_OSMI2_2hA HEK293_TMG_2hB MARCHF8 protein_coding protein_coding 10.48946 10.07817 10.78917 0.5705277 0.463167 0.09825536 8.0551269 8.5849325 7.7497286 0.3316712 0.2533921 -0.1474801 0.76782500 0.85366667 0.71896667 -0.13470000 0.0009419769 0.0009419769 FALSE TRUE
ENST00000453424 ENSG00000165406 HEK293_OSMI2_2hA HEK293_TMG_2hB MARCHF8 protein_coding protein_coding 10.48946 10.07817 10.78917 0.5705277 0.463167 0.09825536 0.6913200 0.5473002 0.7710347 0.1592758 0.1169419 0.4869319 0.06565833 0.05363333 0.07243333 0.01880000 0.7154883053 0.0009419769 FALSE TRUE
ENST00000453980 ENSG00000165406 HEK293_OSMI2_2hA HEK293_TMG_2hB MARCHF8 protein_coding protein_coding 10.48946 10.07817 10.78917 0.5705277 0.463167 0.09825536 0.4903447 0.2485724 0.9032003 0.2485724 0.4926907 1.8203632 0.04489167 0.02750000 0.08113333 0.05363333 0.5705571967 0.0009419769   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000165406 E001 1.0360631 0.1002721838 1.761877e-02 4.630977e-02 10 45454585 45454592 8 - 0.000 0.491 14.761
ENSG00000165406 E002 886.7539661 0.0088363932 5.356896e-08 5.737169e-07 10 45454593 45457505 2913 - 2.811 3.050 0.795
ENSG00000165406 E003 40.8452037 0.0006178616 4.894849e-01 6.269069e-01 10 45457506 45457510 5 - 1.593 1.657 0.217
ENSG00000165406 E004 166.9496395 0.0013426497 4.763488e-01 6.149764e-01 10 45457511 45458117 607 - 2.184 2.264 0.268
ENSG00000165406 E005 91.4884097 0.0003691762 7.070601e-03 2.140946e-02 10 45458118 45458276 159 - 1.966 1.970 0.015
ENSG00000165406 E006 115.8839635 0.0035204650 2.462196e-03 8.650243e-03 10 45458277 45458543 267 - 2.079 2.052 -0.091
ENSG00000165406 E007 70.4180468 0.0107352093 1.194598e-02 3.340314e-02 10 45459120 45459267 148 - 1.883 1.822 -0.204
ENSG00000165406 E008 0.0000000       10 45461083 45461230 148 -      
ENSG00000165406 E009 61.2110240 0.0027057256 5.202331e-04 2.235553e-03 10 45461231 45461386 156 - 1.831 1.758 -0.244
ENSG00000165406 E010 32.9304439 0.0006664195 6.818594e-03 2.075087e-02 10 45461387 45461411 25 - 1.562 1.500 -0.211
ENSG00000165406 E011 1.8518427 0.0434707783 7.202916e-01 8.149277e-01 10 45461412 45462204 793 - 0.399 0.537 0.698
ENSG00000165406 E012 11.9108422 0.0170379265 1.033638e-02 2.959790e-02 10 45463151 45463908 758 - 1.205 1.018 -0.671
ENSG00000165406 E013 4.2558592 0.0784393582 2.386373e-01 3.725638e-01 10 45463909 45463996 88 - 0.785 0.655 -0.536
ENSG00000165406 E014 51.5245180 0.0005172539 1.156249e-02 3.249536e-02 10 45464239 45464327 89 - 1.729 1.708 -0.072
ENSG00000165406 E015 1.4318415 0.0427776090 6.635595e-01 7.715731e-01 10 45484865 45484933 69 - 0.398 0.382 -0.094
ENSG00000165406 E016 46.3002442 0.0004676183 1.643050e-04 8.106013e-04 10 45489367 45489417 51 - 1.719 1.629 -0.306
ENSG00000165406 E017 80.7666265 0.0031665851 4.287075e-08 4.687870e-07 10 45533110 45533289 180 - 1.982 1.826 -0.524
ENSG00000165406 E018 0.0000000       10 45535211 45535371 161 -      
ENSG00000165406 E019 20.9700502 0.0021813858 1.419072e-02 3.865739e-02 10 45553112 45553204 93 - 1.383 1.294 -0.309
ENSG00000165406 E020 5.3553914 0.0252720700 8.006425e-03 2.379433e-02 10 45590524 45590574 51 - 0.930 0.621 -1.242
ENSG00000165406 E021 0.1451727 0.0430203334 4.599386e-01   10 45593430 45593624 195 - 0.114 0.000 -12.008
ENSG00000165406 E022 79.4726563 0.0052607564 2.909323e-05 1.736546e-04 10 45594235 45594906 672 - 1.960 1.846 -0.384