ENSG00000165355

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000298097 ENSG00000165355 HEK293_OSMI2_2hA HEK293_TMG_2hB FBXO33 protein_coding protein_coding 8.943475 2.97284 16.27468 0.5230555 0.322811 2.448757 6.5311038 2.7429290 11.5356866 0.4150735 0.6335800 2.068315 0.80613333 0.93 0.70786667 -0.22213333 3.260937e-02 1.149881e-12 FALSE TRUE
ENST00000554190 ENSG00000165355 HEK293_OSMI2_2hA HEK293_TMG_2hB FBXO33 protein_coding protein_coding 8.943475 2.97284 16.27468 0.5230555 0.322811 2.448757 0.5603584 0.2299111 0.7822025 0.1405076 0.1357408 1.723370 0.07224167 0.07 0.04796667 -0.02203333 8.940730e-01 1.149881e-12 FALSE FALSE
MSTRG.9453.2 ENSG00000165355 HEK293_OSMI2_2hA HEK293_TMG_2hB FBXO33 protein_coding   8.943475 2.97284 16.27468 0.5230555 0.322811 2.448757 1.7787005 0.0000000 3.9205044 0.0000000 0.3918156 8.618571 0.11577917 0.00 0.24203333 0.24203333 1.149881e-12 1.149881e-12 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000165355 E001 376.44612 0.0016377564 1.793304e-34 4.235962e-32 14 39397684 39399565 1882 - 2.411 2.685 0.912
ENSG00000165355 E002 121.06527 0.0035548915 1.199632e-02 3.352264e-02 14 39399566 39399787 222 - 2.002 1.915 -0.292
ENSG00000165355 E003 195.52066 0.0003406173 3.738140e-14 1.129653e-12 14 39401176 39401697 522 - 2.229 2.017 -0.710
ENSG00000165355 E004 83.35834 0.0008806773 1.632854e-06 1.294632e-05 14 39401698 39401861 164 - 1.866 1.648 -0.738
ENSG00000165355 E005 54.07830 0.0004890283 3.298766e-02 7.778126e-02 14 39402401 39402511 111 - 1.663 1.571 -0.313
ENSG00000165355 E006 61.63539 0.0105101897 3.347142e-01 4.784478e-01 14 39431564 39431771 208 - 1.707 1.673 -0.114
ENSG00000165355 E007 40.88875 0.0048837010 3.413243e-04 1.545554e-03 14 39431772 39431970 199 - 1.564 1.298 -0.918
ENSG00000165355 E008 28.76402 0.0079024874 7.623900e-04 3.123187e-03 14 39431971 39432260 290 - 1.422 1.101 -1.127
ENSG00000165355 E009 12.45190 0.0016270430 6.690930e-01 7.759786e-01 14 39432261 39432466 206 - 1.033 1.018 -0.054