ENSG00000165338

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000298068 ENSG00000165338 HEK293_OSMI2_2hA HEK293_TMG_2hB HECTD2 protein_coding protein_coding 2.03197 0.6946671 3.82863 0.1167727 0.1433021 2.445578 1.13781341 0.3020110 1.8513444 0.06322526 0.1134765 2.576676 0.57629583 0.46740000 0.4829667 0.01556667 0.946450997 0.007089596 FALSE TRUE
ENST00000371667 ENSG00000165338 HEK293_OSMI2_2hA HEK293_TMG_2hB HECTD2 protein_coding protein_coding 2.03197 0.6946671 3.82863 0.1167727 0.1433021 2.445578 0.01629795 0.0630414 0.0000000 0.06304140 0.0000000 -2.868714 0.01823750 0.07096667 0.0000000 -0.07096667 0.295835993 0.007089596 FALSE TRUE
ENST00000371681 ENSG00000165338 HEK293_OSMI2_2hA HEK293_TMG_2hB HECTD2 protein_coding protein_coding 2.03197 0.6946671 3.82863 0.1167727 0.1433021 2.445578 0.55962619 0.3236550 1.2403578 0.08407467 0.0500674 1.905912 0.29956250 0.45420000 0.3251667 -0.12903333 0.276493788 0.007089596 FALSE TRUE
ENST00000631422 ENSG00000165338 HEK293_OSMI2_2hA HEK293_TMG_2hB HECTD2 protein_coding protein_coding 2.03197 0.6946671 3.82863 0.1167727 0.1433021 2.445578 0.22714417 0.0000000 0.5443065 0.00000000 0.1309245 5.792612 0.07389167 0.00000000 0.1399333 0.13993333 0.007089596 0.007089596   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000165338 E001 0.8921407 0.0136968632 3.163375e-01 4.590954e-01 10 91409280 91409421 142 + 0.270 0.000 -9.990
ENSG00000165338 E002 0.4460135 0.0314233661 8.640792e-01 9.163445e-01 10 91410285 91410343 59 + 0.156 0.000 -11.253
ENSG00000165338 E003 7.0254795 0.0023240157 1.662963e-01 2.833800e-01 10 91410344 91410576 233 + 0.769 0.960 0.734
ENSG00000165338 E004 14.1772223 0.0013627556 8.298376e-01 8.931815e-01 10 91425281 91425410 130 + 1.081 1.107 0.095
ENSG00000165338 E005 19.6438897 0.0010490851 3.016265e-01 4.434340e-01 10 91460427 91460565 139 + 1.232 1.132 -0.358
ENSG00000165338 E006 16.2439846 0.0012657179 2.274981e-01 3.594168e-01 10 91461254 91461356 103 + 1.157 1.025 -0.476
ENSG00000165338 E007 14.9914456 0.0013508828 5.121894e-03 1.626020e-02 10 91462095 91462184 90 + 1.152 0.790 -1.354
ENSG00000165338 E008 28.4214706 0.0104193640 3.859089e-03 1.274694e-02 10 91462185 91463664 1480 + 1.315 1.558 0.839
ENSG00000165338 E009 8.6409454 0.0062134355 2.359572e-01 3.694879e-01 10 91478201 91478265 65 + 0.913 0.735 -0.695
ENSG00000165338 E010 7.2143244 0.1034050539 7.151701e-01 8.111624e-01 10 91481094 91481139 46 + 0.830 0.744 -0.342
ENSG00000165338 E011 11.4905390 0.0045717085 8.115823e-02 1.613464e-01 10 91482967 91483076 110 + 1.032 0.790 -0.917
ENSG00000165338 E012 0.4513240 0.1670225594 4.626328e-01 6.024953e-01 10 91484090 91484107 18 + 0.110 0.238 1.345
ENSG00000165338 E013 0.7437457 0.0157042467 8.322974e-01 8.949267e-01 10 91484108 91484333 226 + 0.197 0.240 0.361
ENSG00000165338 E014 0.1451727 0.0435937846 1.000000e+00   10 91484334 91484345 12 + 0.058 0.000 -9.806
ENSG00000165338 E015 0.2966881 0.0271656821 1.000000e+00   10 91484346 91484506 161 + 0.110 0.000 -10.764
ENSG00000165338 E016 10.4834041 0.0017793229 2.304968e-02 5.785134e-02 10 91484507 91484655 149 + 1.008 0.671 -1.316
ENSG00000165338 E017 8.4178512 0.0020248718 1.101082e-02 3.118735e-02 10 91485180 91485303 124 + 0.935 0.507 -1.780
ENSG00000165338 E018 4.0780495 0.0040703011 3.732182e-02 8.607558e-02 10 91485304 91487681 2378 + 0.675 0.240 -2.338
ENSG00000165338 E019 5.1757277 0.0031279002 1.988705e-01 3.247928e-01 10 91487682 91487778 97 + 0.736 0.507 -1.007
ENSG00000165338 E020 2.6175367 0.0058670635 8.887087e-01 9.328217e-01 10 91487779 91489981 2203 + 0.478 0.507 0.140
ENSG00000165338 E021 6.4926861 0.0026862835 6.466879e-01 7.585104e-01 10 91491200 91491307 108 + 0.769 0.839 0.275
ENSG00000165338 E022 8.1328269 0.0020404037 5.202018e-01 6.542045e-01 10 91492352 91492484 133 + 0.881 0.790 -0.354
ENSG00000165338 E023 8.0618504 0.0021330897 8.456380e-01 9.040069e-01 10 91493420 91493508 89 + 0.864 0.839 -0.097
ENSG00000165338 E024 10.7054155 0.0016655373 4.882464e-02 1.070689e-01 10 91496214 91496372 159 + 1.014 0.735 -1.075
ENSG00000165338 E025 7.1745304 0.0155587253 5.246391e-01 6.581116e-01 10 91498108 91498182 75 + 0.838 0.735 -0.406
ENSG00000165338 E026 6.7744111 0.1256019677 8.181930e-01 8.851003e-01 10 91498872 91498959 88 + 0.790 0.842 0.206
ENSG00000165338 E027 7.1169819 0.0513291599 6.595588e-01 7.683452e-01 10 91499044 91499150 107 + 0.810 0.880 0.274
ENSG00000165338 E028 7.3502853 0.0023448459 4.160743e-01 5.594364e-01 10 91500502 91500617 116 + 0.856 0.734 -0.480
ENSG00000165338 E029 11.3425053 0.0015300128 7.814113e-01 8.595751e-01 10 91501191 91501334 144 + 0.989 1.025 0.132
ENSG00000165338 E030 70.2925134 0.0005984013 8.636324e-06 5.822414e-05 10 91512264 91514829 2566 + 1.706 1.903 0.664