ENSG00000165238

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000411624 ENSG00000165238 HEK293_OSMI2_2hA HEK293_TMG_2hB WNK2 protein_coding protein_coding 21.91193 35.32409 12.71786 2.136549 1.351612 -1.473072 1.5809593 2.4984872 0.6626998 0.07064590 0.1399801 -1.8987828 0.06731250 0.07146667 0.05483333 -0.016633333 6.688148e-01 1.720339e-15 FALSE TRUE
ENST00000427277 ENSG00000165238 HEK293_OSMI2_2hA HEK293_TMG_2hB WNK2 protein_coding protein_coding 21.91193 35.32409 12.71786 2.136549 1.351612 -1.473072 0.5239074 0.2631715 1.6782348 0.26317150 0.0690191 2.6276367 0.03960833 0.00680000 0.13586667 0.129066667 1.335449e-02 1.720339e-15 FALSE TRUE
ENST00000432730 ENSG00000165238 HEK293_OSMI2_2hA HEK293_TMG_2hB WNK2 protein_coding protein_coding 21.91193 35.32409 12.71786 2.136549 1.351612 -1.473072 2.0017997 3.5650986 0.5330491 0.25327918 0.2696971 -2.7188284 0.08192917 0.10153333 0.03893333 -0.062600000 4.249210e-01 1.720339e-15 FALSE TRUE
ENST00000453718 ENSG00000165238 HEK293_OSMI2_2hA HEK293_TMG_2hB WNK2 protein_coding protein_coding 21.91193 35.32409 12.71786 2.136549 1.351612 -1.473072 1.1784758 2.3102060 0.0000000 0.19194638 0.0000000 -7.8581091 0.04564167 0.06590000 0.00000000 -0.065900000 1.720339e-15 1.720339e-15 FALSE TRUE
ENST00000460335 ENSG00000165238 HEK293_OSMI2_2hA HEK293_TMG_2hB WNK2 protein_coding processed_transcript 21.91193 35.32409 12.71786 2.136549 1.351612 -1.473072 3.7521708 9.4292301 0.4572767 1.21229591 0.4572767 -4.3363201 0.13361667 0.27303333 0.02990000 -0.243133333 4.559772e-02 1.720339e-15   FALSE
ENST00000467401 ENSG00000165238 HEK293_OSMI2_2hA HEK293_TMG_2hB WNK2 protein_coding retained_intron 21.91193 35.32409 12.71786 2.136549 1.351612 -1.473072 2.5187184 3.6978782 1.2434209 0.16658618 0.1608052 -1.5647229 0.11480000 0.10490000 0.10193333 -0.002966667 9.334829e-01 1.720339e-15 FALSE TRUE
ENST00000471076 ENSG00000165238 HEK293_OSMI2_2hA HEK293_TMG_2hB WNK2 protein_coding retained_intron 21.91193 35.32409 12.71786 2.136549 1.351612 -1.473072 1.1691134 1.2075841 0.7063186 0.09676303 0.0648869 -0.7653482 0.05543750 0.03453333 0.05606667 0.021533333 1.587960e-01 1.720339e-15   FALSE
ENST00000478583 ENSG00000165238 HEK293_OSMI2_2hA HEK293_TMG_2hB WNK2 protein_coding processed_transcript 21.91193 35.32409 12.71786 2.136549 1.351612 -1.473072 1.3819372 2.0293057 1.3657123 2.02930574 0.3491252 -0.5678993 0.07538333 0.05236667 0.10406667 0.051700000 1.767127e-01 1.720339e-15 FALSE FALSE
ENST00000693671 ENSG00000165238 HEK293_OSMI2_2hA HEK293_TMG_2hB WNK2 protein_coding processed_transcript 21.91193 35.32409 12.71786 2.136549 1.351612 -1.473072 0.6529952 0.0000000 1.1904703 0.00000000 0.6791357 6.9074559 0.04525000 0.00000000 0.08470000 0.084700000 8.745395e-02 1.720339e-15 FALSE TRUE
MSTRG.33011.19 ENSG00000165238 HEK293_OSMI2_2hA HEK293_TMG_2hB WNK2 protein_coding   21.91193 35.32409 12.71786 2.136549 1.351612 -1.473072 2.7623401 5.8247998 1.5461715 0.76805499 0.3310469 -1.9066821 0.11332500 0.16490000 0.12026667 -0.044633333 4.989195e-01 1.720339e-15 FALSE TRUE
MSTRG.33011.9 ENSG00000165238 HEK293_OSMI2_2hA HEK293_TMG_2hB WNK2 protein_coding   21.91193 35.32409 12.71786 2.136549 1.351612 -1.473072 1.3335116 1.6124024 1.1322470 0.39837747 0.2834343 -0.5062570 0.07137917 0.04460000 0.09493333 0.050333333 3.838291e-01 1.720339e-15 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000165238 E001 0.0000000       9 93184122 93184138 17 +      
ENSG00000165238 E002 0.3289534 0.0296295697 5.583864e-01   9 93184139 93184145 7 + 0.173 0.085 -1.170
ENSG00000165238 E003 2.1808858 0.0582123200 5.745362e-03 1.794273e-02 9 93184146 93184157 12 + 0.770 0.271 -2.496
ENSG00000165238 E004 2.9109019 0.0066561752 2.336921e-05 1.426481e-04 9 93184158 93184165 8 + 0.920 0.271 -3.078
ENSG00000165238 E005 7.0191233 0.0073840699 1.717429e-09 2.436437e-08 9 93184166 93184211 46 + 1.246 0.502 -2.933
ENSG00000165238 E006 8.2671841 0.0034177573 2.044637e-10 3.419709e-09 9 93184212 93184247 36 + 1.302 0.582 -2.754
ENSG00000165238 E007 11.8894476 0.0058310809 3.228158e-10 5.227050e-09 9 93184248 93184361 114 + 1.413 0.778 -2.317
ENSG00000165238 E008 6.1635408 0.0026313711 1.344796e-06 1.086429e-05 9 93184362 93184385 24 + 1.152 0.557 -2.341
ENSG00000165238 E009 4.8244563 0.0032680519 3.279934e-04 1.492790e-03 9 93184928 93184929 2 + 1.012 0.530 -1.959
ENSG00000165238 E010 89.2693208 0.0059141214 9.120953e-13 2.254615e-11 9 93184930 93185610 681 + 2.134 1.763 -1.247
ENSG00000165238 E011 0.0000000       9 93204975 93205003 29 +      
ENSG00000165238 E012 92.8474924 0.0020412954 2.421526e-09 3.341473e-08 9 93229696 93229868 173 + 2.085 1.828 -0.863
ENSG00000165238 E013 108.4455715 0.0058778253 1.289188e-09 1.872521e-08 9 93230888 93231108 221 + 2.179 1.878 -1.010
ENSG00000165238 E014 0.3268771 0.0296709280 5.581563e-01   9 93234776 93234807 32 + 0.173 0.085 -1.170
ENSG00000165238 E015 89.9281782 0.0051369069 2.031817e-08 2.366273e-07 9 93234808 93234922 115 + 2.087 1.804 -0.950
ENSG00000165238 E016 58.9659766 0.0023763270 2.465086e-07 2.321551e-06 9 93234923 93234965 43 + 1.898 1.629 -0.909
ENSG00000165238 E017 76.3991493 0.0027607932 2.234769e-08 2.581684e-07 9 93238233 93238321 89 + 2.009 1.741 -0.902
ENSG00000165238 E018 111.7378953 0.0128779672 1.665828e-04 8.205209e-04 9 93239757 93239976 220 + 2.154 1.921 -0.781
ENSG00000165238 E019 98.0417583 0.0054602140 5.838152e-05 3.234167e-04 9 93247543 93247834 292 + 2.074 1.874 -0.673
ENSG00000165238 E020 56.9160700 0.0225843117 1.573835e-02 4.214429e-02 9 93252883 93253082 200 + 1.838 1.643 -0.660
ENSG00000165238 E021 27.4099530 0.0155030236 2.360448e-02 5.899546e-02 9 93256299 93256454 156 + 1.528 1.330 -0.685
ENSG00000165238 E022 0.1482932 0.0425961285 1.000000e+00   9 93256726 93256769 44 + 0.000 0.085 9.315
ENSG00000165238 E023 40.9191069 0.0008936216 1.596071e-02 4.264209e-02 9 93256948 93257139 192 + 1.662 1.518 -0.492
ENSG00000165238 E024 111.3855210 0.0029434219 2.135107e-02 5.432887e-02 9 93258931 93259614 684 + 2.065 1.965 -0.336
ENSG00000165238 E025 103.3559682 0.0062723853 7.429731e-03 2.233553e-02 9 93261814 93262107 294 + 2.060 1.922 -0.463
ENSG00000165238 E026 56.7772637 0.0084894595 7.406043e-02 1.500254e-01 9 93262670 93262719 50 + 1.789 1.673 -0.392
ENSG00000165238 E027 0.1515154 0.0429554124 2.187036e-01   9 93263266 93263324 59 + 0.173 0.000 -11.579
ENSG00000165238 E028 92.1072724 0.0144999265 6.347595e-01 7.491670e-01 9 93263566 93263734 169 + 1.934 1.908 -0.086
ENSG00000165238 E029 90.7644573 0.0170966001 6.112012e-01 7.303761e-01 9 93263917 93264033 117 + 1.929 1.902 -0.091
ENSG00000165238 E030 0.0000000       9 93267020 93267244 225 +      
ENSG00000165238 E031 119.4515310 0.0021022166 8.946775e-01 9.367028e-01 9 93267746 93267916 171 + 2.027 2.021 -0.021
ENSG00000165238 E032 66.0617706 0.0078996526 3.449327e-01 4.890287e-01 9 93268020 93268065 46 + 1.722 1.785 0.212
ENSG00000165238 E033 72.9274895 0.0019534466 3.632203e-01 5.076509e-01 9 93268627 93268746 120 + 1.782 1.825 0.145
ENSG00000165238 E034 0.4481018 0.2096018135 9.070848e-01 9.450053e-01 9 93268840 93268950 111 + 0.173 0.154 -0.205
ENSG00000165238 E035 142.3514732 0.0002883413 5.000245e-03 1.593033e-02 9 93288788 93289186 399 + 2.029 2.121 0.307
ENSG00000165238 E036 194.2367562 0.0035730199 3.474720e-03 1.165213e-02 9 93289187 93289620 434 + 2.144 2.263 0.399
ENSG00000165238 E037 87.2110120 0.0004635996 2.231120e-02 5.632115e-02 9 93289978 93290047 70 + 1.819 1.915 0.321
ENSG00000165238 E038 36.9728617 0.0034059217 9.646440e-01 9.817000e-01 9 93292308 93292310 3 + 1.534 1.528 -0.021
ENSG00000165238 E039 105.7223594 0.0003574427 9.703642e-01 9.854453e-01 9 93292311 93292396 86 + 1.977 1.972 -0.017
ENSG00000165238 E040 319.6156666 0.0002044873 7.305374e-03 2.201338e-02 9 93292491 93293132 642 + 2.405 2.461 0.189
ENSG00000165238 E041 76.7839624 0.0003462977 1.622623e-04 8.016719e-04 9 93293133 93293173 41 + 1.697 1.873 0.592
ENSG00000165238 E042 165.5199150 0.0002061702 9.364421e-07 7.816971e-06 9 93297853 93298067 215 + 2.045 2.199 0.515
ENSG00000165238 E043 78.4096083 0.0007815553 1.300878e-05 8.413184e-05 9 93299070 93299261 192 + 1.680 1.889 0.706
ENSG00000165238 E044 36.5845156 0.0062565232 2.965518e-01 4.377876e-01 9 93300051 93300149 99 + 1.467 1.542 0.257
ENSG00000165238 E045 10.9043023 0.0532054701 3.979091e-01 5.416551e-01 9 93300150 93300326 177 + 0.894 1.048 0.572
ENSG00000165238 E046 15.8603759 0.0013279945 5.345545e-01 6.665904e-01 9 93306637 93306776 140 + 1.137 1.191 0.195
ENSG00000165238 E047 33.2735102 0.0175631578 1.332471e-02 3.667639e-02 9 93306777 93306821 45 + 1.302 1.541 0.824
ENSG00000165238 E048 117.7901658 0.0033545317 2.120082e-04 1.015302e-03 9 93306822 93308327 1506 + 1.890 2.062 0.576
ENSG00000165238 E049 178.2654486 0.0051341542 3.331355e-07 3.052848e-06 9 93308328 93308500 173 + 2.013 2.252 0.801
ENSG00000165238 E050 123.1129926 0.0014360271 5.997559e-15 2.041357e-13 9 93308501 93308584 84 + 1.764 2.105 1.146
ENSG00000165238 E051 94.7451330 0.0213674827 5.559646e-05 3.096361e-04 9 93308721 93309195 475 + 1.653 1.999 1.168
ENSG00000165238 E052 58.3473869 0.0041595397 2.440743e-01 3.789876e-01 9 93315488 93317519 2032 + 1.768 1.698 -0.236
ENSG00000165238 E053 109.2680290 0.0101169986 1.492179e-04 7.436834e-04 9 93317520 93317631 112 + 1.799 2.041 0.813
ENSG00000165238 E054 20.9791059 0.0023408157 1.927393e-01 3.172086e-01 9 93317632 93317872 241 + 1.208 1.317 0.383
ENSG00000165238 E055 33.9354601 0.0141882639 6.799180e-01 7.839783e-01 9 93317873 93318027 155 + 1.467 1.500 0.113
ENSG00000165238 E056 227.6434619 0.0083655729 1.058772e-05 6.997424e-05 9 93318028 93319208 1181 + 2.127 2.357 0.769
ENSG00000165238 E057 66.1618732 0.0087773047 4.256720e-02 9.575877e-02 9 93320367 93320572 206 + 1.667 1.805 0.467