ENSG00000165169

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000378578 ENSG00000165169 HEK293_OSMI2_2hA HEK293_TMG_2hB DYNLT3 protein_coding protein_coding 10.56925 5.227514 16.78096 0.5540316 1.13656 1.68073 5.9113550 3.3823379 9.7540304 0.49388306 0.48506734 1.525197 0.55203750 0.6708333 0.5827667 -0.08806667 7.193154e-01 2.352898e-07 FALSE FALSE
ENST00000378581 ENSG00000165169 HEK293_OSMI2_2hA HEK293_TMG_2hB DYNLT3 protein_coding protein_coding 10.56925 5.227514 16.78096 0.5540316 1.13656 1.68073 0.3224864 0.6345095 0.1381321 0.06812599 0.04192142 -2.121318 0.05777917 0.1268000 0.0085000 -0.11830000 2.352898e-07 2.352898e-07 FALSE TRUE
MSTRG.34085.3 ENSG00000165169 HEK293_OSMI2_2hA HEK293_TMG_2hB DYNLT3 protein_coding   10.56925 5.227514 16.78096 0.5540316 1.13656 1.68073 4.1558161 1.0002663 6.5775690 1.00026629 0.63043789 2.705011 0.36572500 0.1601000 0.3908667 0.23076667 1.743279e-01 2.352898e-07 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000165169 E001 7.582606 0.0318736724 5.951339e-11 1.089337e-09 X 37836757 37837359 603 - 0.349 1.295 3.921
ENSG00000165169 E002 74.633009 0.0441033313 5.202117e-02 1.127558e-01 X 37838836 37839363 528 - 1.766 1.991 0.758
ENSG00000165169 E003 20.907777 0.0011300341 5.642198e-01 6.915521e-01 X 37839364 37839391 28 - 1.295 1.354 0.206
ENSG00000165169 E004 214.307023 0.0025861988 4.727150e-04 2.055792e-03 X 37839392 37840526 1135 - 2.333 2.244 -0.297
ENSG00000165169 E005 20.982715 0.0146846222 2.856815e-02 6.914631e-02 X 37840527 37840542 16 - 1.377 1.158 -0.771
ENSG00000165169 E006 43.396348 0.0264708992 3.084604e-01 4.507524e-01 X 37840543 37840612 70 - 1.649 1.560 -0.305
ENSG00000165169 E007 46.689152 0.0005028625 6.260646e-01 7.420691e-01 X 37840613 37840651 39 - 1.658 1.645 -0.042
ENSG00000165169 E008 59.662776 0.0005438636 7.924758e-01 8.673175e-01 X 37841028 37841105 78 - 1.753 1.779 0.089
ENSG00000165169 E009 78.708473 0.0004495376 8.465772e-01 9.046572e-01 X 37841782 37841905 124 - 1.873 1.895 0.075
ENSG00000165169 E010 50.598490 0.0008319686 6.079621e-01 7.278384e-01 X 37846317 37846358 42 - 1.679 1.719 0.137
ENSG00000165169 E011 3.542984 0.0046324165 4.752973e-01 6.140131e-01 X 37847043 37847480 438 - 0.589 0.709 0.514
ENSG00000165169 E012 39.604221 0.0005193233 7.092370e-01 8.066739e-01 X 37847481 37847571 91 - 1.573 1.609 0.120