ENSG00000165118

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000314700 ENSG00000165118 HEK293_OSMI2_2hA HEK293_TMG_2hB C9orf64 protein_coding protein_coding 14.34609 10.43469 22.42842 1.615693 0.8316149 1.103201 0.4952759 1.72140043 0.6789504 0.86855786 0.2167465 -1.329467 0.04134583 0.161733333 0.03096667 -0.130766667 0.7047194704 0.0002625487 FALSE TRUE
ENST00000376344 ENSG00000165118 HEK293_OSMI2_2hA HEK293_TMG_2hB C9orf64 protein_coding protein_coding 14.34609 10.43469 22.42842 1.615693 0.8316149 1.103201 12.5983192 8.33675405 17.8497898 1.42473086 0.4308964 1.097428 0.88265000 0.801633333 0.79700000 -0.004633333 0.9108886560 0.0002625487 FALSE TRUE
MSTRG.32913.3 ENSG00000165118 HEK293_OSMI2_2hA HEK293_TMG_2hB C9orf64 protein_coding   14.34609 10.43469 22.42842 1.615693 0.8316149 1.103201 0.9592540 0.07735894 3.4533633 0.07735894 0.7887116 5.309075 0.04991667 0.005666667 0.15196667 0.146300000 0.0002625487 0.0002625487 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000165118 E001 0.4751703 0.0206846117 2.821979e-01 4.221688e-01 9 83938311 83938313 3 - 0.091 0.282 1.979
ENSG00000165118 E002 345.5899827 0.0113080293 9.145148e-04 3.665160e-03 9 83938314 83939364 1051 - 2.449 2.634 0.616
ENSG00000165118 E003 246.5739495 0.0001967251 9.751790e-01 9.885402e-01 9 83939365 83939743 379 - 2.371 2.411 0.132
ENSG00000165118 E004 186.8205766 0.0015182044 2.987526e-03 1.022717e-02 9 83944794 83944989 196 - 2.283 2.228 -0.184
ENSG00000165118 E005 2.4647842 0.0064516380 6.032320e-02 1.271578e-01 9 83953648 83953843 196 - 0.378 0.709 1.564
ENSG00000165118 E006 169.9901759 0.0076091901 2.955419e-01 4.366710e-01 9 83955381 83955544 164 - 2.229 2.205 -0.079
ENSG00000165118 E007 113.4177851 0.0009056877 3.840798e-02 8.811612e-02 9 83955545 83955640 96 - 2.061 2.025 -0.119
ENSG00000165118 E008 96.2790960 0.0003529658 7.917412e-01 8.667911e-01 9 83956164 83956254 91 - 1.968 1.998 0.101
ENSG00000165118 E009 173.9442350 0.0003441804 8.028110e-06 5.450282e-05 9 83956255 83956742 488 - 2.265 2.183 -0.273
ENSG00000165118 E010 2.7473814 0.1961774384 2.528905e-01 3.893233e-01 9 83956897 83956986 90 - 0.629 0.384 -1.194