ENSG00000164975

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000380821 ENSG00000164975 HEK293_OSMI2_2hA HEK293_TMG_2hB SNAPC3 protein_coding protein_coding 9.940835 5.382155 13.75365 0.327139 0.2690377 1.35193 2.0079575 1.2424777 1.712560 0.13367840 0.03682658 0.4597696 0.2200333 0.23043333 0.12453333 -0.10590000 0.0009274537 0.0009274537 FALSE TRUE
ENST00000490969 ENSG00000164975 HEK293_OSMI2_2hA HEK293_TMG_2hB SNAPC3 protein_coding nonsense_mediated_decay 9.940835 5.382155 13.75365 0.327139 0.2690377 1.35193 5.7188762 3.5208468 8.716818 0.29775548 0.33277391 1.3054415 0.5657958 0.65246667 0.63410000 -0.01836667 0.8722488099 0.0009274537 TRUE TRUE
ENST00000610884 ENSG00000164975 HEK293_OSMI2_2hA HEK293_TMG_2hB SNAPC3 protein_coding protein_coding 9.940835 5.382155 13.75365 0.327139 0.2690377 1.35193 1.1173986 0.3680371 1.766276 0.01701672 0.07060798 2.2322563 0.1133167 0.06886667 0.12846667 0.05960000 0.0224538150 0.0009274537 TRUE TRUE
MSTRG.32425.6 ENSG00000164975 HEK293_OSMI2_2hA HEK293_TMG_2hB SNAPC3 protein_coding   9.940835 5.382155 13.75365 0.327139 0.2690377 1.35193 0.8143252 0.2015978 1.094937 0.08777135 0.31692394 2.3845677 0.0750750 0.03880000 0.07936667 0.04056667 0.3911810252 0.0009274537 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000164975 E001 2.0044789 0.0074066800 9.880859e-01 9.965975e-01 9 15422734 15422868 135 + 0.472 0.468 -0.019
ENSG00000164975 E002 3.8584865 0.0278269912 6.659572e-01 7.735125e-01 9 15422869 15422875 7 + 0.653 0.727 0.312
ENSG00000164975 E003 4.0067797 0.0570516792 5.947982e-01 7.169709e-01 9 15422876 15422878 3 + 0.653 0.762 0.450
ENSG00000164975 E004 15.7884371 0.0122978432 8.390740e-02 1.656610e-01 9 15422879 15422893 15 + 1.266 1.084 -0.650
ENSG00000164975 E005 60.6530587 0.0028734483 2.986965e-01 4.401546e-01 9 15422894 15423141 248 + 1.793 1.735 -0.197
ENSG00000164975 E006 39.0217793 0.0093465135 6.016316e-01 7.227477e-01 9 15423142 15423193 52 + 1.600 1.555 -0.154
ENSG00000164975 E007 58.5905299 0.0109420038 2.570632e-01 3.941057e-01 9 15423909 15423986 78 + 1.785 1.696 -0.302
ENSG00000164975 E008 68.3967345 0.0127137093 3.080151e-01 4.502545e-01 9 15433552 15433636 85 + 1.846 1.766 -0.271
ENSG00000164975 E009 0.5202097 0.0207135772 9.340243e-01 9.624227e-01 9 15433637 15433824 188 + 0.157 0.172 0.155
ENSG00000164975 E010 79.2888986 0.0004252322 2.461546e-02 6.108626e-02 9 15444602 15444706 105 + 1.915 1.817 -0.331
ENSG00000164975 E011 46.1604891 0.0005233793 3.565690e-02 8.291610e-02 9 15447095 15447111 17 + 1.692 1.573 -0.407
ENSG00000164975 E012 75.3447354 0.0003609652 2.726868e-01 4.116388e-01 9 15447112 15447244 133 + 1.881 1.832 -0.166
ENSG00000164975 E013 60.2821873 0.0004067287 6.081060e-01 7.279496e-01 9 15451320 15451402 83 + 1.781 1.754 -0.090
ENSG00000164975 E014 84.5270907 0.0005209494 1.826221e-01 3.044883e-01 9 15453041 15453205 165 + 1.935 1.879 -0.189
ENSG00000164975 E015 0.1472490 0.0438170989 1.000000e+00   9 15453206 15453577 372 + 0.086 0.000 -7.648
ENSG00000164975 E016 70.4940420 0.0051171106 1.406220e-01 2.488736e-01 9 15457960 15458067 108 + 1.865 1.784 -0.272
ENSG00000164975 E017 175.2479602 0.0015959559 1.369148e-01 2.437899e-01 9 15459719 15460313 595 + 2.213 2.254 0.138
ENSG00000164975 E018 57.4940512 0.0006382258 1.729778e-14 5.512216e-13 9 15460314 15461626 1313 + 1.598 1.948 1.183
ENSG00000164975 E019 86.1267295 0.0004504653 3.363017e-01 4.801265e-01 9 15461631 15461805 175 + 1.909 1.944 0.116
ENSG00000164975 E020 110.3565484 0.0015842565 2.973198e-05 1.771066e-04 9 15461806 15462460 655 + 1.971 2.122 0.508
ENSG00000164975 E021 3.5192398 0.0146540315 4.226088e-01 5.657450e-01 9 15462847 15462864 18 + 0.673 0.536 -0.610
ENSG00000164975 E022 8.2149992 0.1603190793 6.970637e-01 7.972942e-01 9 15462865 15462948 84 + 0.970 0.877 -0.351
ENSG00000164975 E023 6.6272872 0.2557961147 8.013284e-01 8.734135e-01 9 15462949 15462990 42 + 0.881 0.816 -0.249
ENSG00000164975 E024 6.2310476 0.0196740368 8.296019e-02 1.641776e-01 9 15462991 15463327 337 + 0.914 0.644 -1.082
ENSG00000164975 E025 18.8133059 0.0075277323 4.018053e-02 9.137165e-02 9 15465510 15465953 444 + 1.337 1.135 -0.715