ENSG00000164934

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000521716 ENSG00000164934 HEK293_OSMI2_2hA HEK293_TMG_2hB DCAF13 protein_coding retained_intron 40.01305 17.48342 64.86028 0.9454007 1.215531 1.890745 1.621413 0.0000000 3.611217 0.0000000 0.8929323 8.500331 0.02955833 0.0000 0.0562000 0.0562000 5.230796e-09 5.230796e-09 FALSE TRUE
ENST00000521999 ENSG00000164934 HEK293_OSMI2_2hA HEK293_TMG_2hB DCAF13 protein_coding protein_coding 40.01305 17.48342 64.86028 0.9454007 1.215531 1.890745 2.269908 0.3615175 3.554977 0.2405295 0.1110950 3.262390 0.05671667 0.0197 0.0549000 0.0352000 2.982646e-01 5.230796e-09 FALSE FALSE
ENST00000612750 ENSG00000164934 HEK293_OSMI2_2hA HEK293_TMG_2hB DCAF13 protein_coding protein_coding 40.01305 17.48342 64.86028 0.9454007 1.215531 1.890745 34.246513 16.5247305 54.232720 0.9057918 2.1622476 1.713930 0.86634583 0.9453 0.8354667 -0.1098333 2.589285e-02 5.230796e-09 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000164934 E001 13.6903483 0.0016153221 1.383440e-01 2.457994e-01 8 103414714 103415364 651 + 1.176 1.017 -0.574
ENSG00000164934 E002 5.1941998 0.0065773180 5.075835e-01 6.430433e-01 8 103415365 103415378 14 + 0.790 0.687 -0.419
ENSG00000164934 E003 6.1498389 0.0677343892 9.138795e-01 9.494838e-01 8 103415379 103415384 6 + 0.829 0.813 -0.062
ENSG00000164934 E004 6.1455725 0.0830066104 9.042121e-01 9.430342e-01 8 103415385 103415385 1 + 0.829 0.813 -0.063
ENSG00000164934 E005 41.7626527 0.0005470262 1.163233e-01 2.147133e-01 8 103415386 103415411 26 + 1.562 1.650 0.302
ENSG00000164934 E006 289.1755527 0.0012025074 1.117564e-03 4.361927e-03 8 103415412 103415516 105 + 2.449 2.351 -0.326
ENSG00000164934 E007 481.0540690 0.0018238361 3.643542e-04 1.636031e-03 8 103420264 103420463 200 + 2.670 2.571 -0.331
ENSG00000164934 E008 0.7406253 0.0153999473 1.812986e-01 3.028710e-01 8 103420464 103420648 185 + 0.292 0.001 -9.023
ENSG00000164934 E009 301.2096290 0.0047482173 9.640794e-02 1.849707e-01 8 103420975 103421082 108 + 2.461 2.387 -0.247
ENSG00000164934 E010 157.2575499 0.0012049536 1.599895e-01 2.751060e-01 8 103426056 103426056 1 + 2.177 2.124 -0.180
ENSG00000164934 E011 309.3006685 0.0016134506 5.006067e-02 1.092747e-01 8 103426057 103426145 89 + 2.471 2.409 -0.207
ENSG00000164934 E012 382.9228008 0.0001670477 7.871223e-01 8.636685e-01 8 103427097 103427252 156 + 2.549 2.540 -0.028
ENSG00000164934 E013 81.8583551 0.0199709157 4.853942e-07 4.303064e-06 8 103427253 103430611 3359 + 1.971 1.514 -1.546
ENSG00000164934 E014 273.7657694 0.0001705298 3.311080e-01 4.746970e-01 8 103430612 103430689 78 + 2.394 2.414 0.065
ENSG00000164934 E015 245.2833151 0.0001868704 1.900022e-02 4.933302e-02 8 103432659 103432741 83 + 2.337 2.392 0.182
ENSG00000164934 E016 340.8477413 0.0023819447 2.762259e-02 6.726157e-02 8 103435626 103435790 165 + 2.475 2.539 0.214
ENSG00000164934 E017 3.3298669 0.0058510752 1.433352e-03 5.418098e-03 8 103439482 103440135 654 + 0.404 0.883 2.114
ENSG00000164934 E018 401.4873613 0.0057458408 2.522618e-01 3.885859e-01 8 103440136 103440271 136 + 2.552 2.597 0.149
ENSG00000164934 E019 2.8776891 0.0468107651 3.982080e-01 5.419462e-01 8 103440272 103441454 1183 + 0.492 0.640 0.675
ENSG00000164934 E020 423.6030061 0.0014165274 2.071207e-03 7.444846e-03 8 103441455 103441618 164 + 2.567 2.641 0.244
ENSG00000164934 E021 2.9838494 0.0276957583 2.256724e-02 5.685764e-02 8 103441619 103442794 1176 + 0.435 0.812 1.673
ENSG00000164934 E022 281.3292535 0.0011125073 7.878755e-07 6.691202e-06 8 103442795 103443453 659 + 2.372 2.501 0.430