ENSG00000164548

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000297071 ENSG00000164548 HEK293_OSMI2_2hA HEK293_TMG_2hB TRA2A protein_coding protein_coding 75.57183 63.14532 83.29991 10.01478 0.9921019 0.3995839 54.727293 55.0491652 53.622985 8.6731861 1.1664431 -0.0378622 0.73231667 0.872033333 0.64390000 -0.22813333 7.535040e-26 7.53504e-26 FALSE TRUE
ENST00000494255 ENSG00000164548 HEK293_OSMI2_2hA HEK293_TMG_2hB TRA2A protein_coding retained_intron 75.57183 63.14532 83.29991 10.01478 0.9921019 0.3995839 6.332970 1.6835818 8.866369 0.1568471 0.1245951 2.3898920 0.08691667 0.028566667 0.10643333 0.07786667 1.100041e-05 7.53504e-26 FALSE FALSE
MSTRG.29580.16 ENSG00000164548 HEK293_OSMI2_2hA HEK293_TMG_2hB TRA2A protein_coding   75.57183 63.14532 83.29991 10.01478 0.9921019 0.3995839 2.810183 0.4050198 4.878489 0.4050198 0.2482691 3.5581366 0.03050833 0.007566667 0.05860000 0.05103333 6.514272e-02 7.53504e-26   FALSE
MSTRG.29580.9 ENSG00000164548 HEK293_OSMI2_2hA HEK293_TMG_2hB TRA2A protein_coding   75.57183 63.14532 83.29991 10.01478 0.9921019 0.3995839 2.673257 0.4883336 5.966917 0.2546656 0.6793275 3.5842177 0.03267083 0.007866667 0.07146667 0.06360000 3.476450e-02 7.53504e-26 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000164548 E001 3.721260 0.0043392506 1.182004e-02 3.310328e-02 7 23504780 23504783 4 - 0.421 0.813 1.749
ENSG00000164548 E002 590.877013 0.0008578433 4.365690e-31 7.906949e-29 7 23504784 23505197 414 - 2.631 2.869 0.791
ENSG00000164548 E003 322.070038 0.0031161422 3.518926e-22 2.851369e-20 7 23505198 23505248 51 - 2.299 2.633 1.112
ENSG00000164548 E004 332.649616 0.0023148933 3.564020e-35 8.826050e-33 7 23505249 23505315 67 - 2.273 2.661 1.293
ENSG00000164548 E005 206.172625 0.0003621301 2.114372e-42 8.486471e-40 7 23505316 23505319 4 - 2.078 2.458 1.269
ENSG00000164548 E006 511.946776 0.0001732514 2.377877e-75 4.210108e-72 7 23505320 23505467 148 - 2.515 2.834 1.060
ENSG00000164548 E007 531.909471 0.0028313137 1.846765e-04 8.978701e-04 7 23505468 23505569 102 - 2.663 2.776 0.376
ENSG00000164548 E008 6.280891 0.0803683317 9.974937e-02 1.900957e-01 7 23505570 23505745 176 - 0.994 0.733 -1.009
ENSG00000164548 E009 346.695628 0.0021143434 2.103635e-01 3.389732e-01 7 23505746 23505810 65 - 2.522 2.561 0.129
ENSG00000164548 E010 230.098376 0.0012720223 8.355943e-01 8.971784e-01 7 23505811 23505813 3 - 2.369 2.367 -0.007
ENSG00000164548 E011 1.846633 0.1307014881 8.899131e-01 9.336414e-01 7 23505814 23505885 72 - 0.471 0.462 -0.047
ENSG00000164548 E012 289.647473 0.0006170164 1.864546e-01 3.093071e-01 7 23506138 23506263 126 - 2.481 2.457 -0.080
ENSG00000164548 E013 27.394789 0.0007897324 2.654824e-02 6.505101e-02 7 23506264 23506266 3 - 1.532 1.397 -0.466
ENSG00000164548 E014 3.285940 0.0047801775 1.751533e-03 6.445287e-03 7 23506267 23506480 214 - 0.838 0.361 -2.186
ENSG00000164548 E015 5.669949 0.0211220972 1.393358e-02 3.807241e-02 7 23507157 23507419 263 - 0.978 0.627 -1.395
ENSG00000164548 E016 30.581955 0.0006405148 1.848373e-03 6.753287e-03 7 23507420 23507420 1 - 1.598 1.413 -0.632
ENSG00000164548 E017 534.467906 0.0001627301 5.419446e-15 1.854584e-13 7 23507421 23507535 115 - 2.792 2.678 -0.381
ENSG00000164548 E018 698.132924 0.0001999420 1.254069e-19 7.661389e-18 7 23512894 23513024 131 - 2.911 2.791 -0.400
ENSG00000164548 E019 450.200865 0.0001650622 9.496533e-09 1.176337e-07 7 23513025 23513082 58 - 2.706 2.617 -0.298
ENSG00000164548 E020 397.548729 0.0002611601 1.586509e-07 1.550474e-06 7 23516363 23516394 32 - 2.652 2.562 -0.302
ENSG00000164548 E021 649.725372 0.0002809594 1.040951e-17 5.100409e-16 7 23516395 23516528 134 - 2.881 2.756 -0.414
ENSG00000164548 E022 566.905294 0.0032839323 1.773592e-04 8.669669e-04 7 23521707 23521840 134 - 2.816 2.695 -0.404
ENSG00000164548 E023 61.749378 0.0231833867 1.745168e-08 2.057995e-07 7 23521841 23522120 280 - 2.005 1.483 -1.766
ENSG00000164548 E024 28.268217 0.0006849669 3.502637e-10 5.637648e-09 7 23522121 23522131 11 - 1.645 1.235 -1.416
ENSG00000164548 E025 95.492311 0.0003500176 2.246839e-45 1.072184e-42 7 23522132 23522353 222 - 2.200 1.660 -1.817
ENSG00000164548 E026 56.270422 0.0397378393 3.921877e-05 2.265345e-04 7 23522354 23522432 79 - 1.952 1.482 -1.594
ENSG00000164548 E027 18.318973 0.0131254269 1.528459e-03 5.729022e-03 7 23531181 23531202 22 - 1.429 1.123 -1.075
ENSG00000164548 E028 22.279455 0.0066770675 8.659595e-06 5.836331e-05 7 23531203 23531270 68 - 1.532 1.164 -1.282
ENSG00000164548 E029 10.902034 0.0177053404 1.457710e-11 2.954835e-10 7 23531417 23531788 372 - 1.361 0.468 -3.503
ENSG00000164548 E030 515.742714 0.0022673579 6.967962e-08 7.293953e-07 7 23531789 23532041 253 - 2.789 2.640 -0.497