ENSG00000164494

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000369031 ENSG00000164494 HEK293_OSMI2_2hA HEK293_TMG_2hB PDSS2 protein_coding protein_coding 7.4322 5.308529 8.302655 0.4162705 0.6810393 0.6442822 1.510402 2.262189 1.328261 0.1530946 0.3656728 -0.7637236 0.2623625 0.4285000 0.1546667 -0.2738333 0.001835862 0.001835862 FALSE TRUE
ENST00000369037 ENSG00000164494 HEK293_OSMI2_2hA HEK293_TMG_2hB PDSS2 protein_coding protein_coding 7.4322 5.308529 8.302655 0.4162705 0.6810393 0.6442822 4.859935 2.913874 5.793669 0.3397565 0.2158785 0.9890840 0.6243792 0.5467667 0.7057333 0.1589667 0.197161568 0.001835862 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000164494 E001 170.6427315 0.0049927851 2.493128e-03 8.744156e-03 6 107152562 107153905 1344 - 2.177 2.299 0.407
ENSG00000164494 E002 71.9225122 0.0003628776 1.121705e-01 2.087154e-01 6 107153906 107154389 484 - 1.880 1.829 -0.172
ENSG00000164494 E003 28.6493149 0.0053063461 1.269819e-01 2.299445e-01 6 107154390 107154460 71 - 1.508 1.409 -0.342
ENSG00000164494 E004 69.6612101 0.0045901803 7.105400e-04 2.936618e-03 6 107154461 107154777 317 - 1.907 1.742 -0.555
ENSG00000164494 E005 24.8828453 0.0204241936 2.702880e-01 4.088994e-01 6 107193822 107193854 33 - 1.446 1.348 -0.336
ENSG00000164494 E006 0.1515154 0.0429863703 7.646742e-01   6 107197841 107197898 58 - 0.098 0.000 -7.959
ENSG00000164494 E007 35.3756660 0.0134816747 1.836933e-01 3.058359e-01 6 107210439 107210570 132 - 1.597 1.497 -0.344
ENSG00000164494 E008 34.2304180 0.0065723783 3.388676e-02 7.952808e-02 6 107212109 107212282 174 - 1.597 1.456 -0.484
ENSG00000164494 E009 23.6829992 0.0008385280 4.294948e-04 1.888029e-03 6 107245548 107245619 72 - 1.476 1.227 -0.865
ENSG00000164494 E010 23.4461870 0.0145320553 1.457093e-10 2.502873e-09 6 107264186 107264512 327 - 1.037 1.632 2.083
ENSG00000164494 E011 63.0907242 0.0004570357 4.032780e-01 5.470553e-01 6 107274029 107274227 199 - 1.784 1.834 0.167
ENSG00000164494 E012 44.8055104 0.0034786042 5.130811e-01 6.479253e-01 6 107334198 107334332 135 - 1.639 1.692 0.179
ENSG00000164494 E013 88.2229317 0.0039561600 7.968473e-01 8.703604e-01 6 107458990 107459367 378 - 1.947 1.945 -0.004
ENSG00000164494 E014 35.5864217 0.0055266288 5.241667e-01 6.577934e-01 6 107459368 107459623 256 - 1.537 1.590 0.182