ENSG00000164463

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000296953 ENSG00000164463 HEK293_OSMI2_2hA HEK293_TMG_2hB CREBRF protein_coding protein_coding 1.307549 0.3537353 2.408551 0.09606382 0.2413801 2.733182 0.42002096 0.17808792 0.75578627 0.005320963 0.13498833 2.0255346 0.44812500 0.57786667 0.30940000 -0.26846667 0.19255842 0.01214175 FALSE TRUE
ENST00000517882 ENSG00000164463 HEK293_OSMI2_2hA HEK293_TMG_2hB CREBRF protein_coding processed_transcript 1.307549 0.3537353 2.408551 0.09606382 0.2413801 2.733182 0.08444979 0.07641404 0.24809775 0.076414045 0.15989706 1.5785799 0.06515417 0.14103333 0.10666667 -0.03436667 1.00000000 0.01214175   FALSE
ENST00000520420 ENSG00000164463 HEK293_OSMI2_2hA HEK293_TMG_2hB CREBRF protein_coding protein_coding 1.307549 0.3537353 2.408551 0.09606382 0.2413801 2.733182 0.02507128 0.05045636 0.02846044 0.005524466 0.01459252 -0.6525189 0.06292500 0.15470000 0.01170000 -0.14300000 0.01214175 0.01214175 FALSE TRUE
ENST00000520464 ENSG00000164463 HEK293_OSMI2_2hA HEK293_TMG_2hB CREBRF protein_coding retained_intron 1.307549 0.3537353 2.408551 0.09606382 0.2413801 2.733182 0.02459315 0.02372865 0.03658594 0.007715438 0.01305675 0.4659198 0.04301667 0.06523333 0.01503333 -0.05020000 0.43638565 0.01214175 TRUE TRUE
ENST00000523161 ENSG00000164463 HEK293_OSMI2_2hA HEK293_TMG_2hB CREBRF protein_coding protein_coding 1.307549 0.3537353 2.408551 0.09606382 0.2413801 2.733182 0.07902603 0.00000000 0.45414835 0.000000000 0.26772849 5.5365141 0.03014167 0.00000000 0.17403333 0.17403333 0.56471216 0.01214175   FALSE
MSTRG.27318.1 ENSG00000164463 HEK293_OSMI2_2hA HEK293_TMG_2hB CREBRF protein_coding   1.307549 0.3537353 2.408551 0.09606382 0.2413801 2.733182 0.15915136 0.00000000 0.19640653 0.000000000 0.13992945 4.3674167 0.06618333 0.00000000 0.09076667 0.09076667 0.75494740 0.01214175 FALSE TRUE
MSTRG.27318.10 ENSG00000164463 HEK293_OSMI2_2hA HEK293_TMG_2hB CREBRF protein_coding   1.307549 0.3537353 2.408551 0.09606382 0.2413801 2.733182 0.10583352 0.00000000 0.13065234 0.000000000 0.13065234 3.8140617 0.06057500 0.00000000 0.06013333 0.06013333 1.00000000 0.01214175 FALSE TRUE
MSTRG.27318.4 ENSG00000164463 HEK293_OSMI2_2hA HEK293_TMG_2hB CREBRF protein_coding   1.307549 0.3537353 2.408551 0.09606382 0.2413801 2.733182 0.37759356 0.02504836 0.51591256 0.013534026 0.03803670 3.9074041 0.20195000 0.06113333 0.21526667 0.15413333 0.15272875 0.01214175 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000164463 E001 0.1451727 0.0432759444 9.530951e-01   5 173056352 173056367 16 + 0.054 0.000 -7.977
ENSG00000164463 E002 0.4439371 0.0217609444 1.000000e+00 1.000000e+00 5 173056368 173056389 22 + 0.146 0.000 -11.124
ENSG00000164463 E003 2.2089628 0.0836260146 8.629034e-02 1.694633e-01 5 173056390 173056479 90 + 0.476 0.000 -12.701
ENSG00000164463 E004 0.6696487 0.0285487845 5.499617e-01 6.796474e-01 5 173057344 173057393 50 + 0.146 0.255 1.005
ENSG00000164463 E005 1.5511803 0.0091320969 6.972884e-01 7.974452e-01 5 173057394 173057455 62 + 0.341 0.255 -0.582
ENSG00000164463 E006 2.1382138 0.0218771450 4.221658e-01 5.653304e-01 5 173057456 173057550 95 + 0.435 0.255 -1.111
ENSG00000164463 E007 3.4827107 0.0053833380 1.203669e-01 2.204993e-01 5 173057551 173057683 133 + 0.593 0.255 -1.872
ENSG00000164463 E008 0.3729606 0.0281810494 1.924273e-01 3.168107e-01 5 173060252 173060455 204 + 0.054 0.255 2.590
ENSG00000164463 E009 2.5013845 0.0913569227 6.680027e-02 1.380643e-01 5 173080585 173080588 4 + 0.512 0.000 -12.807
ENSG00000164463 E010 7.9419094 0.0359220637 8.717877e-01 9.215405e-01 5 173080589 173080784 196 + 0.851 0.817 -0.133
ENSG00000164463 E011 6.8809773 0.0035679587 8.177910e-01 8.847976e-01 5 173086501 173086626 126 + 0.800 0.762 -0.150
ENSG00000164463 E012 8.4825087 0.0021469323 2.240510e-01 3.552514e-01 5 173090315 173090423 109 + 0.890 0.698 -0.763
ENSG00000164463 E013 25.8184627 0.0008079932 3.915831e-02 8.947563e-02 5 173090424 173091223 800 + 1.337 1.139 -0.699
ENSG00000164463 E014 10.5613015 0.0025183850 1.312262e-01 2.358584e-01 5 173091224 173091401 178 + 0.984 0.762 -0.851
ENSG00000164463 E015 0.1472490 0.0433494204 9.566397e-01   5 173091402 173092330 929 + 0.054 0.000 -9.704
ENSG00000164463 E016 8.2590878 0.0042905650 1.082073e-01 2.028894e-01 5 173108624 173108818 195 + 0.890 0.622 -1.085
ENSG00000164463 E017 0.2966881 0.0275509960 1.000000e+00   5 173108819 173110521 1703 + 0.102 0.000 -10.627
ENSG00000164463 E018 6.6496019 0.0026094118 6.158526e-01 7.340134e-01 5 173110522 173110711 190 + 0.781 0.698 -0.338
ENSG00000164463 E019 5.1359723 0.0033164753 7.246589e-01 8.181238e-01 5 173112306 173112379 74 + 0.686 0.623 -0.270
ENSG00000164463 E020 6.8173506 0.0029052419 2.743178e-01 4.134540e-01 5 173123080 173123202 123 + 0.809 0.622 -0.770
ENSG00000164463 E021 0.5483223 0.0210015019 3.690881e-02 8.528501e-02 5 173123203 173124581 1379 + 0.054 0.415 3.590
ENSG00000164463 E022 6.9472099 0.0023863756 9.694281e-02 1.858164e-01 5 173133630 173133765 136 + 0.827 0.531 -1.254
ENSG00000164463 E023 79.1547216 0.0008570145 3.609247e-02 8.376448e-02 5 173133766 173138821 5056 + 1.765 1.847 0.278
ENSG00000164463 E024 9.9760499 0.0096510125 3.188716e-10 5.168486e-09 5 173138822 173139284 463 + 0.709 1.397 2.539