ENSG00000164346

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000514918 ENSG00000164346 HEK293_OSMI2_2hA HEK293_TMG_2hB NSA2 protein_coding retained_intron 106.2839 51.98959 179.2419 5.029756 5.085853 1.785416 7.134134 0.2816894 16.32719 0.2816894 2.269831 5.807583 0.04490417 0.006033333 0.09123333 0.08520000 2.507169e-02 1.468435e-05 FALSE  
MSTRG.26400.5 ENSG00000164346 HEK293_OSMI2_2hA HEK293_TMG_2hB NSA2 protein_coding   106.2839 51.98959 179.2419 5.029756 5.085853 1.785416 90.209589 47.0483071 149.81747 4.4153682 5.040000 1.670781 0.86712083 0.905400000 0.83566667 -0.06973333 1.468435e-05 1.468435e-05 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000164346 E001 4.018016 0.0476054185 6.028123e-01 7.237163e-01 5 74766991 74767247 257 + 0.640 0.725 0.356
ENSG00000164346 E002 3.796570 0.0297977693 8.156503e-01 8.833189e-01 5 74767248 74767248 1 + 0.640 0.677 0.157
ENSG00000164346 E003 53.742645 0.0086860235 5.929753e-03 1.842382e-02 5 74767249 74767259 11 + 1.759 1.548 -0.715
ENSG00000164346 E004 59.691170 0.0100081390 2.542915e-02 6.276515e-02 5 74767260 74767262 3 + 1.795 1.622 -0.586
ENSG00000164346 E005 485.814355 0.0024844839 8.265076e-09 1.034620e-07 5 74767263 74767363 101 + 2.702 2.525 -0.591
ENSG00000164346 E006 994.209259 0.0010674647 5.622600e-09 7.273368e-08 5 74768931 74769118 188 + 2.997 2.885 -0.370
ENSG00000164346 E007 765.480255 0.0000991446 2.770893e-04 1.286359e-03 5 74769214 74769297 84 + 2.868 2.817 -0.169
ENSG00000164346 E008 630.219916 0.0009910569 1.560409e-01 2.698471e-01 5 74769298 74769364 67 + 2.778 2.746 -0.108
ENSG00000164346 E009 5.777053 0.0066306982 4.754078e-01 6.141133e-01 5 74769365 74769554 190 + 0.829 0.723 -0.422
ENSG00000164346 E010 1063.538652 0.0001718417 2.473107e-14 7.675667e-13 5 74770631 74770810 180 + 2.967 3.055 0.294
ENSG00000164346 E011 1.477825 0.1169163571 2.729728e-01 4.119478e-01 5 74771477 74771653 177 + 0.299 0.556 1.394
ENSG00000164346 E012 15.811281 0.0012490047 7.222803e-02 1.470430e-01 5 74772313 74772421 109 + 1.136 1.295 0.564
ENSG00000164346 E013 993.941631 0.0008274831 3.883320e-12 8.648994e-11 5 74773868 74774060 193 + 2.929 3.040 0.367
ENSG00000164346 E014 1.743380 0.0276552117 5.168171e-01 6.513291e-01 5 74774916 74775037 122 + 0.444 0.317 -0.732
ENSG00000164346 E015 632.949189 0.0048514316 1.482626e-01 2.593144e-01 5 74776604 74779308 2705 + 2.754 2.804 0.166
ENSG00000164346 E016 6.488412 0.0029019195 1.035024e-04 5.382142e-04 5 74779309 74780113 805 + 0.969 0.316 -2.958