ENSG00000164323

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000458385 ENSG00000164323 HEK293_OSMI2_2hA HEK293_TMG_2hB CFAP97 protein_coding protein_coding 6.317997 1.402078 10.14524 0.1210273 0.4042356 2.846333 3.335988 1.3576292 4.637580 0.1401800 0.1618472 1.764802 0.6868125 0.96553333 0.4573333 -0.5082 9.249299e-22 3.41315e-22 FALSE TRUE
MSTRG.25816.2 ENSG00000164323 HEK293_OSMI2_2hA HEK293_TMG_2hB CFAP97 protein_coding   6.317997 1.402078 10.14524 0.1210273 0.4042356 2.846333 2.868943 0.0146264 5.330968 0.0146264 0.3086849 7.760751 0.2799208 0.01076667 0.5247667 0.5140 3.413150e-22 3.41315e-22 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000164323 E001 128.3755144 0.0243349858 5.457408e-13 1.396016e-11 4 185159665 185161289 1625 - 1.804 2.409 2.026
ENSG00000164323 E002 236.0234706 0.0002344792 1.584597e-01 2.730728e-01 4 185161290 185162925 1636 - 2.208 2.285 0.259
ENSG00000164323 E003 51.3810844 0.0023048386 6.556611e-04 2.736512e-03 4 185164029 185164179 151 - 1.584 1.438 -0.502
ENSG00000164323 E004 0.3299976 0.0274424043 1.010193e-02   4 185169026 185170340 1315 - 0.000 0.441 13.562
ENSG00000164323 E005 25.2076836 0.0007582464 1.563948e-02 4.193290e-02 4 185175786 185175799 14 - 1.288 1.152 -0.479
ENSG00000164323 E006 71.0828707 0.0004294176 2.055147e-03 7.396578e-03 4 185175800 185176051 252 - 1.713 1.645 -0.231
ENSG00000164323 E007 0.1515154 0.0428998978 1.000000e+00   4 185182178 185182519 342 - 0.049 0.000 -8.780
ENSG00000164323 E008 125.3329745 0.0071459441 5.239812e-04 2.249312e-03 4 185190143 185190767 625 - 1.958 1.866 -0.310
ENSG00000164323 E009 74.8523092 0.0004001213 4.151092e-13 1.081722e-11 4 185190768 185191212 445 - 1.764 1.378 -1.320
ENSG00000164323 E010 5.5317390 0.0029562174 1.190666e-02 3.331017e-02 4 185203785 185203897 113 - 0.727 0.274 -2.298
ENSG00000164323 E011 13.7110795 0.0153841607 5.102611e-04 2.197986e-03 4 185203898 185204028 131 - 1.068 0.562 -2.016
ENSG00000164323 E012 0.1472490 0.0435677182 1.000000e+00   4 185206777 185207082 306 - 0.049 0.000 -8.772
ENSG00000164323 E013 0.2944980 0.3918417152 1.000000e+00   4 185207735 185207791 57 - 0.094 0.000 -8.153
ENSG00000164323 E014 1.3661505 0.0583570075 2.737568e-02 6.675644e-02 4 185209325 185209504 180 - 0.171 0.657 2.879