ENSG00000164284

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000329271 ENSG00000164284 HEK293_OSMI2_2hA HEK293_TMG_2hB GRPEL2 protein_coding protein_coding 15.35156 7.200549 23.90639 2.320223 0.1580884 1.729819 11.082466 3.13153925 17.834133 0.7568836 0.93737703 2.505906 0.61374167 0.45530000 0.74570000 0.29040000 2.249968e-04 5.518373e-14 FALSE TRUE
ENST00000416916 ENSG00000164284 HEK293_OSMI2_2hA HEK293_TMG_2hB GRPEL2 protein_coding protein_coding 15.35156 7.200549 23.90639 2.320223 0.1580884 1.729819 0.673201 0.07793996 1.618914 0.0370753 0.23291283 4.211248 0.03279583 0.01483333 0.06776667 0.05293333 1.372205e-02 5.518373e-14 FALSE FALSE
MSTRG.27138.1 ENSG00000164284 HEK293_OSMI2_2hA HEK293_TMG_2hB GRPEL2 protein_coding   15.35156 7.200549 23.90639 2.320223 0.1580884 1.729819 2.543193 3.81181394 2.397760 1.6417229 0.04804864 -0.666566 0.29376667 0.49616667 0.10030000 -0.39586667 5.518373e-14 5.518373e-14 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000164284 E001 4.328538 0.0050478908 2.919675e-01 4.329023e-01 5 149345430 149345498 69 + 0.684 0.563 -0.529
ENSG00000164284 E002 4.182333 0.0037961884 6.312453e-01 7.462947e-01 5 149345499 149345501 3 + 0.654 0.635 -0.082
ENSG00000164284 E003 62.261122 0.0031470649 5.429377e-02 1.167606e-01 5 149345502 149345616 115 + 1.739 1.698 -0.137
ENSG00000164284 E004 6.300259 0.0093008112 4.357856e-01 5.779572e-01 5 149345619 149345825 207 + 0.808 0.752 -0.223
ENSG00000164284 E005 115.980683 0.0034451140 1.088791e-02 3.089883e-02 5 149348272 149348425 154 + 2.006 1.957 -0.167
ENSG00000164284 E006 90.592435 0.0037798521 1.214463e-03 4.689789e-03 5 149349654 149349735 82 + 1.911 1.812 -0.336
ENSG00000164284 E007 2.113121 0.0239529341 1.429916e-01 2.521181e-01 5 149349736 149349858 123 + 0.488 0.221 -1.645
ENSG00000164284 E008 193.381385 0.0003150739 2.477784e-05 1.504486e-04 5 149350918 149351351 434 + 2.227 2.178 -0.164
ENSG00000164284 E009 612.513526 0.0033024006 3.949456e-12 8.778747e-11 5 149351352 149353436 2085 + 2.734 2.627 -0.354
ENSG00000164284 E010 443.375974 0.0068498966 2.626668e-20 1.736874e-18 5 149353437 149354583 1147 + 2.462 2.824 1.207