ENSG00000164182

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000296597 ENSG00000164182 HEK293_OSMI2_2hA HEK293_TMG_2hB NDUFAF2 protein_coding protein_coding 16.99229 13.01495 22.63314 1.395697 0.840931 0.7977965 12.5786973 9.0392160 17.119623 1.1144328 0.7505771 0.9206287 0.73278333 0.6924000 0.75603333 0.063633333 0.10125971 0.02528688 FALSE  
ENST00000678452 ENSG00000164182 HEK293_OSMI2_2hA HEK293_TMG_2hB NDUFAF2 protein_coding nonsense_mediated_decay 16.99229 13.01495 22.63314 1.395697 0.840931 0.7977965 2.4377800 2.2967615 2.762065 0.2068292 0.1208868 0.2650925 0.14921250 0.1772333 0.12203333 -0.055200000 0.02528688 0.02528688    
MSTRG.26261.2 ENSG00000164182 HEK293_OSMI2_2hA HEK293_TMG_2hB NDUFAF2 protein_coding   16.99229 13.01495 22.63314 1.395697 0.840931 0.7977965 0.8985953 0.7456569 1.258867 0.1227780 0.2533565 0.7477374 0.05514583 0.0573000 0.05576667 -0.001533333 0.97255001 0.02528688 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000164182 E001 2.7907083 0.0081151369 1.282030e-01 0.2316508116 5 60945143 60945159 17 + 0.668 0.418 -1.175
ENSG00000164182 E002 7.3089260 0.0286052465 3.942959e-01 0.5381480621 5 60945160 60945176 17 + 0.971 0.849 -0.464
ENSG00000164182 E003 10.5381697 0.0557549784 6.311986e-01 0.7462566731 5 60945177 60945182 6 + 1.096 1.013 -0.303
ENSG00000164182 E004 54.7810771 0.0037257516 4.439951e-01 0.5855780351 5 60945183 60945204 22 + 1.724 1.767 0.145
ENSG00000164182 E005 56.8804779 0.0031389242 4.532755e-01 0.5940128124 5 60945205 60945206 2 + 1.741 1.782 0.138
ENSG00000164182 E006 137.3293060 0.0091356032 4.762222e-01 0.6148722815 5 60945207 60945222 16 + 2.155 2.112 -0.145
ENSG00000164182 E007 376.1009588 0.0004019576 6.163659e-05 0.0003394537 5 60945223 60945334 112 + 2.604 2.532 -0.240
ENSG00000164182 E008 377.5454009 0.0002281168 1.795893e-04 0.0008762404 5 60945335 60945382 48 + 2.604 2.542 -0.207
ENSG00000164182 E009 44.5008177 0.0005100627 5.867369e-03 0.0182644377 5 60945383 60945428 46 + 1.587 1.736 0.506
ENSG00000164182 E010 5.8364864 0.1156696628 9.462201e-01 0.9702395715 5 61045524 61045604 81 + 0.830 0.845 0.055
ENSG00000164182 E011 8.3710844 0.0020538751 2.052491e-02 0.0525961829 5 61045840 61045978 139 + 0.846 1.105 0.965
ENSG00000164182 E012 0.4439371 0.0215735157 1.763224e-01 0.2964584219 5 61050376 61050540 165 + 0.251 0.000 -11.113
ENSG00000164182 E013 463.1796470 0.0001575561 5.421343e-01 0.6729505048 5 61073125 61073214 90 + 2.670 2.666 -0.014
ENSG00000164182 E014 1.8488108 0.0090947082 6.588763e-01 0.7678669125 5 61083243 61083329 87 + 0.490 0.418 -0.370
ENSG00000164182 E015 272.6937675 0.0001800726 1.713171e-02 0.0452316451 5 61098992 61099032 41 + 2.413 2.468 0.183
ENSG00000164182 E016 0.5514428 0.0201747182 3.613252e-02 0.0838440732 5 61121858 61126086 4229 + 0.000 0.346 12.103
ENSG00000164182 E017 454.1243095 0.0015339317 1.161126e-04 0.0005960972 5 61152704 61154531 1828 + 2.618 2.706 0.295