ENSG00000164169

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000322396 ENSG00000164169 HEK293_OSMI2_2hA HEK293_TMG_2hB PRMT9 protein_coding protein_coding 6.636677 2.057053 10.32267 0.08802936 0.4447707 2.321565 2.5945605 0.7270525 4.259009 0.08588089 0.2560416 2.534062 0.37180417 0.3583333 0.4158333 0.0575000 0.781939932 0.000516703 FALSE TRUE
ENST00000514886 ENSG00000164169 HEK293_OSMI2_2hA HEK293_TMG_2hB PRMT9 protein_coding nonsense_mediated_decay 6.636677 2.057053 10.32267 0.08802936 0.4447707 2.321565 3.0679499 1.3300004 3.991972 0.17351382 0.2681770 1.578477 0.48635833 0.6416667 0.3879333 -0.2537333 0.012905785 0.000516703 FALSE TRUE
MSTRG.25560.4 ENSG00000164169 HEK293_OSMI2_2hA HEK293_TMG_2hB PRMT9 protein_coding   6.636677 2.057053 10.32267 0.08802936 0.4447707 2.321565 0.7344412 0.0000000 1.799987 0.00000000 0.7450166 7.499836 0.08356667 0.0000000 0.1705000 0.1705000 0.000516703 0.000516703 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000164169 E001 17.1551866 0.0042270058 0.4920680341 0.629283593 4 147637785 147638384 600 - 1.189 1.127 -0.221
ENSG00000164169 E002 55.2152100 0.0094358461 0.0053625874 0.016916313 4 147638385 147638680 296 - 1.611 1.810 0.674
ENSG00000164169 E003 37.4365153 0.0484402077 0.1748343859 0.294479302 4 147638681 147638747 67 - 1.449 1.644 0.668
ENSG00000164169 E004 45.1345288 0.0006085887 0.0088643633 0.025942765 4 147638960 147639082 123 - 1.540 1.704 0.559
ENSG00000164169 E005 0.1482932 0.0414638195 0.0954235256   4 147639083 147639293 211 - 0.000 0.227 12.132
ENSG00000164169 E006 45.6736856 0.0005517233 0.1146570723 0.212334927 4 147642787 147642940 154 - 1.562 1.667 0.356
ENSG00000164169 E007 90.8681180 0.0003816767 0.1304011167 0.234722245 4 147653852 147654245 394 - 1.858 1.933 0.252
ENSG00000164169 E008 75.4135818 0.0004306374 0.3105838580 0.452995867 4 147654246 147654566 321 - 1.782 1.840 0.197
ENSG00000164169 E009 49.4976210 0.0005188883 0.4837213652 0.621744525 4 147657792 147657975 184 - 1.622 1.590 -0.110
ENSG00000164169 E010 55.8430185 0.0006101434 0.4715841243 0.610687194 4 147660846 147661038 193 - 1.672 1.641 -0.105
ENSG00000164169 E011 0.2924217 0.0273314735 1.0000000000   4 147661039 147661318 280 - 0.116 0.000 -9.082
ENSG00000164169 E012 47.4528155 0.0004879145 0.0353348706 0.082293556 4 147668539 147668645 107 - 1.622 1.496 -0.432
ENSG00000164169 E013 48.3301371 0.0005040649 0.0031831622 0.010806403 4 147670641 147670743 103 - 1.641 1.456 -0.633
ENSG00000164169 E014 55.9539297 0.0010267343 0.0013998335 0.005306220 4 147672959 147673126 168 - 1.705 1.514 -0.648
ENSG00000164169 E015 58.0268837 0.0004095036 0.0005345393 0.002289034 4 147673638 147673874 237 - 1.723 1.523 -0.678
ENSG00000164169 E016 42.3451027 0.0046689831 0.0096915456 0.028002840 4 147680323 147680471 149 - 1.589 1.399 -0.652
ENSG00000164169 E017 46.5252498 0.0016201691 0.0371858719 0.085824018 4 147683799 147684163 365 - 1.562 1.698 0.461