ENSG00000164031

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000334223 ENSG00000164031 HEK293_OSMI2_2hA HEK293_TMG_2hB DNAJB14 protein_coding nonsense_mediated_decay 9.474655 2.975025 13.53307 0.2222987 0.6457006 2.181739 0.7610538 0.01856809 1.0319854 0.01856809 0.2782841 5.188787 0.05033333 0.005566667 0.07470000 0.06913333 3.601162e-03 4.565137e-06 FALSE TRUE
ENST00000442697 ENSG00000164031 HEK293_OSMI2_2hA HEK293_TMG_2hB DNAJB14 protein_coding protein_coding 9.474655 2.975025 13.53307 0.2222987 0.6457006 2.181739 3.5300205 0.55870342 5.4616463 0.12417162 0.2179774 3.266227 0.33531667 0.196100000 0.40493333 0.20883333 3.366723e-02 4.565137e-06 FALSE TRUE
ENST00000455208 ENSG00000164031 HEK293_OSMI2_2hA HEK293_TMG_2hB DNAJB14 protein_coding nonsense_mediated_decay 9.474655 2.975025 13.53307 0.2222987 0.6457006 2.181739 0.7550277 0.00000000 1.6514347 0.00000000 0.3849925 7.376286 0.04585833 0.000000000 0.12233333 0.12233333 4.565137e-06 4.565137e-06 FALSE FALSE
ENST00000471738 ENSG00000164031 HEK293_OSMI2_2hA HEK293_TMG_2hB DNAJB14 protein_coding processed_transcript 9.474655 2.975025 13.53307 0.2222987 0.6457006 2.181739 1.2180809 1.57391307 0.5525697 0.35162191 0.2140462 -1.493389 0.32381250 0.524466667 0.04046667 -0.48400000 2.218713e-05 4.565137e-06 FALSE FALSE
ENST00000610281 ENSG00000164031 HEK293_OSMI2_2hA HEK293_TMG_2hB DNAJB14 protein_coding protein_coding 9.474655 2.975025 13.53307 0.2222987 0.6457006 2.181739 2.5272400 0.62901837 3.2317195 0.34583241 0.5732826 2.342830 0.16803333 0.209466667 0.23613333 0.02666667 8.665992e-01 4.565137e-06 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000164031 E001 192.7520968 0.0004003740 7.561150e-02 1.524859e-01 4 99896248 99898759 2512 - 2.117 2.189 0.243
ENSG00000164031 E002 45.1547396 0.0005074722 1.135213e-04 5.839970e-04 4 99898760 99899395 636 - 1.541 1.209 -1.153
ENSG00000164031 E003 101.0816613 0.0003164848 5.172945e-06 3.669034e-05 4 99899396 99900634 1239 - 1.875 1.633 -0.817
ENSG00000164031 E004 70.3895633 0.0034165391 1.439767e-04 7.207853e-04 4 99900635 99901152 518 - 1.724 1.458 -0.910
ENSG00000164031 E005 35.0096553 0.0158139985 4.648533e-02 1.029122e-01 4 99903726 99903777 52 - 1.424 1.210 -0.747
ENSG00000164031 E006 48.5740034 0.0090070726 5.311870e-03 1.678119e-02 4 99903778 99903898 121 - 1.565 1.315 -0.862
ENSG00000164031 E007 40.9987953 0.0005013286 6.897991e-06 4.758242e-05 4 99905597 99905706 110 - 1.506 1.069 -1.534
ENSG00000164031 E008 1.6391087 0.0146915822 1.973127e-01 3.228758e-01 4 99906038 99906153 116 - 0.363 0.000 -11.310
ENSG00000164031 E009 30.8820622 0.0010443848 3.221477e-05 1.901599e-04 4 99906517 99906611 95 - 1.389 0.911 -1.715
ENSG00000164031 E010 18.1534852 0.0010699636 7.033278e-03 2.131177e-02 4 99908711 99908728 18 - 1.165 0.804 -1.346
ENSG00000164031 E011 31.3212525 0.0006771159 2.464076e-04 1.160338e-03 4 99908729 99908896 168 - 1.391 0.997 -1.401
ENSG00000164031 E012 0.1472490 0.0458648945 7.573103e-01   4 99910349 99910375 27 - 0.049 0.000 -7.922
ENSG00000164031 E013 2.6969548 0.0073808640 2.786273e-03 9.633373e-03 4 99922520 99923039 520 - 0.340 0.860 2.398
ENSG00000164031 E014 30.6123713 0.0065968846 5.241536e-01 6.577813e-01 4 99923040 99923185 146 - 1.349 1.297 -0.184
ENSG00000164031 E015 6.8575952 0.0380428421 6.879067e-12 1.472064e-10 4 99923681 99923877 197 - 0.425 1.421 3.935
ENSG00000164031 E016 0.2903454 0.3008561679 1.000000e+00   4 99924307 99924759 453 - 0.092 0.000 -7.970
ENSG00000164031 E017 7.7465253 0.0105839362 7.255503e-16 2.792632e-14 4 99924760 99924790 31 - 0.479 1.455 3.772
ENSG00000164031 E018 7.0971418 0.0459993179 1.233241e-07 1.230944e-06 4 99924791 99924815 25 - 0.543 1.343 3.076
ENSG00000164031 E019 15.4347149 0.0463794149 2.415221e-01 3.759768e-01 4 99924816 99927716 2901 - 1.041 1.225 0.661
ENSG00000164031 E020 4.2735206 0.1492636385 1.479277e-01 2.588578e-01 4 99927717 99927838 122 - 0.544 0.852 1.290
ENSG00000164031 E021 1.9711826 0.0154481760 7.289094e-01 8.212439e-01 4 99927839 99928541 703 - 0.363 0.445 0.453
ENSG00000164031 E022 4.7713661 0.0033260013 1.232111e-02 3.430524e-02 4 99928542 99928617 76 - 0.557 0.956 1.623
ENSG00000164031 E023 2.0713900 0.0070476700 7.250032e-01 8.183516e-01 4 99928618 99930449 1832 - 0.362 0.445 0.456
ENSG00000164031 E024 47.2919234 0.0006359132 1.084629e-08 1.328587e-07 4 99930450 99930621 172 - 1.455 1.815 1.226
ENSG00000164031 E025 36.2314545 0.0006421029 2.771410e-09 3.787040e-08 4 99946439 99946618 180 - 1.330 1.744 1.418