ENSG00000163950

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000429429 ENSG00000163950 HEK293_OSMI2_2hA HEK293_TMG_2hB SLBP protein_coding protein_coding 70.57411 58.92485 99.03136 5.911139 2.262457 0.74891 4.667658 11.36856 3.356398 2.6899932 0.7312741 -1.7570408 0.07225833 0.1881333 0.03413333 -0.1540000 2.876683e-06 2.876683e-06 FALSE TRUE
ENST00000489418 ENSG00000163950 HEK293_OSMI2_2hA HEK293_TMG_2hB SLBP protein_coding protein_coding 70.57411 58.92485 99.03136 5.911139 2.262457 0.74891 33.592496 19.65200 57.972980 0.2982664 2.4370027 1.5602196 0.46627083 0.3389333 0.58716667 0.2482333 5.054258e-04 2.876683e-06 FALSE TRUE
MSTRG.24444.4 ENSG00000163950 HEK293_OSMI2_2hA HEK293_TMG_2hB SLBP protein_coding   70.57411 58.92485 99.03136 5.911139 2.262457 0.74891 28.482554 25.98899 31.706240 2.5597013 5.3729783 0.2867664 0.41001667 0.4412000 0.31796667 -0.1232333 1.961879e-01 2.876683e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000163950 E001 11.5745640 4.923341e-02 1.223541e-02 3.409971e-02 4 1692731 1692799 69 - 0.908 1.258 1.271
ENSG00000163950 E002 90.8010348 5.158947e-04 3.595770e-19 2.088313e-17 4 1692800 1692857 58 - 1.795 2.109 1.055
ENSG00000163950 E003 172.1689718 5.136720e-04 5.963747e-23 5.259841e-21 4 1692858 1692959 102 - 2.108 2.362 0.848
ENSG00000163950 E004 920.7202426 8.462847e-05 1.653385e-23 1.550637e-21 4 1692960 1693243 284 - 2.926 3.012 0.286
ENSG00000163950 E005 790.3553958 1.348118e-04 4.683500e-15 1.617639e-13 4 1693244 1693446 203 - 2.866 2.937 0.238
ENSG00000163950 E006 893.3585114 2.010450e-04 2.033756e-08 2.368240e-07 4 1693447 1693713 267 - 2.934 2.976 0.139
ENSG00000163950 E007 568.9258198 1.553073e-04 4.507912e-01 5.918298e-01 4 1694774 1694840 67 - 2.775 2.735 -0.134
ENSG00000163950 E008 591.0700940 1.461874e-04 9.665886e-03 2.794106e-02 4 1696202 1696294 93 - 2.771 2.781 0.033
ENSG00000163950 E009 460.2656704 2.042547e-04 1.610815e-03 5.995719e-03 4 1696295 1696351 57 - 2.655 2.680 0.084
ENSG00000163950 E010 683.9794542 6.216968e-04 4.851606e-01 6.230979e-01 4 1699564 1699701 138 - 2.846 2.826 -0.065
ENSG00000163950 E011 436.3964035 1.500260e-03 6.210222e-01 7.380626e-01 4 1700011 1700070 60 - 2.662 2.617 -0.150
ENSG00000163950 E012 0.9264943 1.428055e-02 6.174361e-01 7.352485e-01 4 1700071 1700091 21 - 0.260 0.335 0.507
ENSG00000163950 E013 494.9029795 5.009035e-04 2.860668e-11 5.528358e-10 4 1703596 1703700 105 - 2.761 2.604 -0.522
ENSG00000163950 E014 253.7521888 2.944688e-03 2.551096e-19 1.509833e-17 4 1711874 1711927 54 - 2.534 2.179 -1.184
ENSG00000163950 E015 208.3066158 3.967270e-03 1.113326e-26 1.397722e-24 4 1711928 1711990 63 - 2.479 1.997 -1.613
ENSG00000163950 E016 4.0081317 4.640586e-03 1.570183e-04 7.784636e-04 4 1711991 1712018 28 - 0.893 0.249 -3.135
ENSG00000163950 E017 169.2945903 2.226696e-02 2.121026e-13 5.755112e-12 4 1712130 1712200 71 - 2.419 1.776 -2.155
ENSG00000163950 E018 132.0250749 3.220153e-02 1.751180e-14 5.568241e-13 4 1712201 1712344 144 - 2.335 1.525 -2.730