ENSG00000163874

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000373087 ENSG00000163874 HEK293_OSMI2_2hA HEK293_TMG_2hB ZC3H12A protein_coding protein_coding 2.694424 3.685158 1.700774 0.1852273 0.08105737 -1.110987 0.9635975 1.2397425 0.6256521 0.92127466 0.14319027 -0.97532153 0.35317083 0.35150000 0.36210000 0.01060000 0.86533626 0.01163543 FALSE  
ENST00000471012 ENSG00000163874 HEK293_OSMI2_2hA HEK293_TMG_2hB ZC3H12A protein_coding protein_coding 2.694424 3.685158 1.700774 0.1852273 0.08105737 -1.110987 0.1701098 0.1584715 0.1704490 0.08024676 0.10292890 0.09908624 0.05385417 0.04213333 0.09826667 0.05613333 0.82453398 0.01163543 FALSE  
ENST00000472312 ENSG00000163874 HEK293_OSMI2_2hA HEK293_TMG_2hB ZC3H12A protein_coding retained_intron 2.694424 3.685158 1.700774 0.1852273 0.08105737 -1.110987 0.3873013 0.3639739 0.1129619 0.10774366 0.02045568 -1.60472662 0.13095000 0.10010000 0.06563333 -0.03446667 0.71909915 0.01163543    
ENST00000640233 ENSG00000163874 HEK293_OSMI2_2hA HEK293_TMG_2hB ZC3H12A protein_coding nonsense_mediated_decay 2.694424 3.685158 1.700774 0.1852273 0.08105737 -1.110987 0.1670651 0.3079447 0.0000000 0.13561768 0.00000000 -4.99070381 0.06555000 0.08556667 0.00000000 -0.08556667 0.01163543 0.01163543 TRUE  
MSTRG.857.1 ENSG00000163874 HEK293_OSMI2_2hA HEK293_TMG_2hB ZC3H12A protein_coding   2.694424 3.685158 1.700774 0.1852273 0.08105737 -1.110987 1.0063500 1.6150249 0.7917108 0.88854570 0.11665847 -1.01930801 0.39647500 0.42070000 0.47400000 0.05330000 0.90637150 0.01163543 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000163874 E001 3.675203 0.0056257482 0.56446319 0.69175195 1 37474536 37474579 44 + 0.539 0.668 0.575
ENSG00000163874 E002 8.730350 0.0019771795 0.48157674 0.61978608 1 37474580 37474629 50 + 0.971 0.932 -0.147
ENSG00000163874 E003 4.729413 0.0106451022 0.88778573 0.93227172 1 37475459 37475461 3 + 0.693 0.708 0.062
ENSG00000163874 E004 32.835544 0.0007639886 0.01195624 0.03342542 1 37475462 37475939 478 + 1.555 1.450 -0.362
ENSG00000163874 E005 2.503404 0.0063556671 0.46761408 0.60711656 1 37479999 37480229 231 + 0.393 0.554 0.810
ENSG00000163874 E006 1.955082 0.0101462472 0.35572533 0.50006750 1 37480230 37480289 60 + 0.297 0.498 1.130
ENSG00000163874 E007 18.974012 0.0010741336 0.15457053 0.26784223 1 37480290 37480429 140 + 1.305 1.234 -0.246
ENSG00000163874 E008 15.363311 0.0577638290 0.52695572 0.66013027 1 37481601 37481689 89 + 1.073 1.196 0.440
ENSG00000163874 E009 19.377737 0.0332277250 0.68743938 0.78978591 1 37481690 37481835 146 + 1.197 1.287 0.317
ENSG00000163874 E010 1.400519 0.0120339672 0.73634316 0.82685573 1 37482350 37482353 4 + 0.393 0.360 -0.192
ENSG00000163874 E011 2.990014 0.1441893068 0.45557708 0.59610124 1 37482354 37482433 80 + 0.393 0.632 1.156
ENSG00000163874 E012 16.732874 0.0950225464 0.69880554 0.79868369 1 37482434 37482540 107 + 1.247 1.185 -0.220
ENSG00000163874 E013 1.298411 0.0107717443 0.04637902 0.10272727 1 37482541 37482602 62 + 0.539 0.217 -1.926
ENSG00000163874 E014 118.634644 0.0508015655 0.30307350 0.44497772 1 37482737 37484377 1641 + 1.932 2.053 0.407