ENSG00000163818

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000296135 ENSG00000163818 HEK293_OSMI2_2hA HEK293_TMG_2hB LZTFL1 protein_coding protein_coding 18.51155 4.719427 32.46548 0.5779725 0.6102843 2.779614 6.282325 2.785993 11.322622 0.3440758 0.7846296 2.019049 0.4058167 0.5966000 0.3487000 -0.24790000 3.660778e-03 3.532718e-38 FALSE TRUE
ENST00000411866 ENSG00000163818 HEK293_OSMI2_2hA HEK293_TMG_2hB LZTFL1 protein_coding nonsense_mediated_decay 18.51155 4.719427 32.46548 0.5779725 0.6102843 2.779614 5.959320 1.651807 9.184291 0.5271509 0.4419305 2.467986 0.3048333 0.3357667 0.2827000 -0.05306667 8.213514e-01 3.532718e-38   FALSE
MSTRG.22853.4 ENSG00000163818 HEK293_OSMI2_2hA HEK293_TMG_2hB LZTFL1 protein_coding   18.51155 4.719427 32.46548 0.5779725 0.6102843 2.779614 5.573487 0.000000 10.945911 0.0000000 0.1117448 10.097494 0.2400208 0.0000000 0.3373667 0.33736667 3.532718e-38 3.532718e-38 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000163818 E001 0.0000000       3 45823316 45823319 4 -      
ENSG00000163818 E002 0.0000000       3 45823320 45823342 23 -      
ENSG00000163818 E003 172.8111438 0.0189811458 1.279518e-05 8.292028e-05 3 45823343 45824775 1433 - 2.056 2.384 1.096
ENSG00000163818 E004 131.8261210 0.0351257250 7.358429e-01 8.265095e-01 3 45824776 45825042 267 - 2.024 2.038 0.049
ENSG00000163818 E005 193.0277662 0.0020968032 3.740717e-01 5.184905e-01 3 45825043 45825965 923 - 2.193 2.171 -0.074
ENSG00000163818 E006 144.3977887 0.0002872318 1.874223e-03 6.835957e-03 3 45825966 45826240 275 - 2.038 2.159 0.406
ENSG00000163818 E007 65.2344690 0.0003883233 1.779937e-02 4.670521e-02 3 45826241 45826255 15 - 1.694 1.829 0.457
ENSG00000163818 E008 75.9123000 0.0003376756 4.838585e-02 1.063024e-01 3 45826256 45826277 22 - 1.765 1.874 0.365
ENSG00000163818 E009 77.5562937 0.0003481419 9.582335e-02 1.841195e-01 3 45826278 45826301 24 - 1.780 1.874 0.314
ENSG00000163818 E010 74.2266157 0.0003302952 7.097381e-01 8.070306e-01 3 45826302 45826332 31 - 1.774 1.805 0.106
ENSG00000163818 E011 1.1844606 0.0108647759 5.885741e-01 7.117689e-01 3 45827128 45827355 228 - 0.274 0.388 0.717
ENSG00000163818 E012 94.4348610 0.0003182233 1.551052e-01 2.685477e-01 3 45827356 45827459 104 - 1.868 1.943 0.253
ENSG00000163818 E013 158.4782650 0.0003275868 6.821200e-01 7.856280e-01 3 45828439 45828615 177 - 2.100 2.127 0.091
ENSG00000163818 E014 91.5044160 0.0002923264 5.442741e-01 6.747613e-01 3 45830913 45830945 33 - 1.872 1.856 -0.053
ENSG00000163818 E015 96.4544081 0.0002708620 1.547628e-01 2.681043e-01 3 45830946 45830990 45 - 1.901 1.848 -0.180
ENSG00000163818 E016 106.7945235 0.0003843017 2.165024e-01 3.461915e-01 3 45831073 45831138 66 - 1.941 1.898 -0.143
ENSG00000163818 E017 0.6060617 0.9223430810 1.000000e+00 1.000000e+00 3 45832580 45832827 248 - 0.202 0.000 -9.160
ENSG00000163818 E018 1.0488529 0.1534985276 8.886552e-01 9.328177e-01 3 45832828 45833049 222 - 0.275 0.235 -0.298
ENSG00000163818 E019 132.1678327 0.0030094771 7.974945e-02 1.591654e-01 3 45833050 45833121 72 - 2.037 1.965 -0.241
ENSG00000163818 E020 130.9252246 0.0190575565 2.154331e-01 3.449475e-01 3 45834238 45834298 61 - 2.035 1.954 -0.269
ENSG00000163818 E021 0.8963053 0.1682750772 4.182638e-01 5.615407e-01 3 45834299 45835589 1291 - 0.201 0.384 1.275
ENSG00000163818 E022 128.6086856 0.0173788041 4.950471e-02 1.082854e-01 3 45835590 45835669 80 - 2.036 1.900 -0.457
ENSG00000163818 E023 126.3106503 0.0002861609 7.447906e-04 3.060171e-03 3 45835670 45835784 115 - 2.028 1.898 -0.437
ENSG00000163818 E024 49.9987144 0.0004340873 4.033798e-02 9.164828e-02 3 45837927 45837940 14 - 1.631 1.506 -0.424
ENSG00000163818 E025 104.4249116 0.0003354787 4.443856e-05 2.535310e-04 3 45837941 45838047 107 - 1.954 1.770 -0.619
ENSG00000163818 E026 52.3662477 0.0304258312 2.143627e-02 5.450764e-02 3 45838048 45838051 4 - 1.669 1.420 -0.852
ENSG00000163818 E027 0.5244761 0.2843407135 4.795642e-01 6.179357e-01 3 45841695 45841988 294 - 0.112 0.240 1.326
ENSG00000163818 E028 79.8537007 0.0168979879 1.054640e-02 3.008378e-02 3 45841989 45842145 157 - 1.843 1.629 -0.725
ENSG00000163818 E029 0.2214452 0.0402332060 8.201396e-02   3 45854411 45854537 127 - 0.000 0.237 11.472
ENSG00000163818 E030 1.6166533 0.0126675238 2.964899e-03 1.015879e-02 3 45854986 45855074 89 - 0.200 0.727 2.888
ENSG00000163818 E031 0.0000000       3 45858940 45859016 77 -      
ENSG00000163818 E032 0.0000000       3 45884663 45884685 23 -      
ENSG00000163818 E033 1.2479590 0.0184709672 5.304919e-03 1.676092e-02 3 45913120 45913177 58 - 0.158 0.663 3.037
ENSG00000163818 E034 0.9911135 0.0127263610 6.901680e-02 1.417424e-01 3 45915421 45916042 622 - 0.158 0.501 2.305