ENSG00000163811

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000296126 ENSG00000163811 HEK293_OSMI2_2hA HEK293_TMG_2hB WDR43 protein_coding protein_coding 42.16895 12.71642 74.56377 1.104621 1.719352 2.550842 5.7413883 0.0000000 9.7293685 0.0000000 1.7124375 9.927684 0.07880417 0.00000000 0.129966667 0.12996667 8.895428e-17 8.895428e-17 FALSE FALSE
ENST00000407426 ENSG00000163811 HEK293_OSMI2_2hA HEK293_TMG_2hB WDR43 protein_coding protein_coding 42.16895 12.71642 74.56377 1.104621 1.719352 2.550842 32.1446518 9.9790483 58.7453823 1.1434613 1.4590434 2.556302 0.80671250 0.80690000 0.789066667 -0.01783333 7.431645e-01 8.895428e-17 FALSE TRUE
ENST00000446643 ENSG00000163811 HEK293_OSMI2_2hA HEK293_TMG_2hB WDR43 protein_coding processed_transcript 42.16895 12.71642 74.56377 1.104621 1.719352 2.550842 0.8750050 1.5045889 0.4293349 1.2271189 0.2051912 -1.785533 0.05206667 0.10500000 0.005766667 -0.09923333 4.910295e-01 8.895428e-17 FALSE TRUE
MSTRG.18145.4 ENSG00000163811 HEK293_OSMI2_2hA HEK293_TMG_2hB WDR43 protein_coding   42.16895 12.71642 74.56377 1.104621 1.719352 2.550842 0.1486669 0.8594957 0.0000000 0.8594957 0.0000000 -6.442107 0.00790000 0.05893333 0.000000000 -0.05893333 5.159140e-01 8.895428e-17 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000163811 E001 211.834247 0.0002730032 3.499916e-09 4.691492e-08 2 28894667 28894923 257 + 2.237 2.080 -0.525
ENSG00000163811 E002 0.000000       2 28895086 28895353 268 +      
ENSG00000163811 E003 0.294498 0.4250576968 1.000000e+00   2 28895804 28895861 58 + 0.106 0.000 -9.064
ENSG00000163811 E004 0.000000       2 28896920 28896998 79 +      
ENSG00000163811 E005 260.975638 0.0005687106 7.588146e-06 5.180739e-05 2 28901987 28902124 138 + 2.319 2.226 -0.310
ENSG00000163811 E006 1.037200 0.0250284535 2.321291e-01 3.649595e-01 2 28905006 28905051 46 + 0.294 0.000 -10.866
ENSG00000163811 E007 295.771620 0.0005056554 1.867249e-07 1.799311e-06 2 28906460 28906581 122 + 2.376 2.269 -0.356
ENSG00000163811 E008 318.782369 0.0002453639 4.487722e-09 5.899149e-08 2 28912590 28912710 121 + 2.410 2.295 -0.381
ENSG00000163811 E009 339.132687 0.0002134821 2.408414e-09 3.326529e-08 2 28914069 28914208 140 + 2.436 2.325 -0.370
ENSG00000163811 E010 250.795745 0.0014079167 8.427751e-03 2.485734e-02 2 28917893 28917995 103 + 2.294 2.251 -0.142
ENSG00000163811 E011 186.271929 0.0005300045 7.233354e-03 2.183074e-02 2 28922919 28922983 65 + 2.167 2.120 -0.158
ENSG00000163811 E012 293.001047 0.0001800963 5.841243e-03 1.819387e-02 2 28924982 28925096 115 + 2.358 2.331 -0.088
ENSG00000163811 E013 194.464458 0.0001964160 2.463226e-01 3.816405e-01 2 28925097 28925153 57 + 2.174 2.185 0.037
ENSG00000163811 E014 295.416790 0.0002966166 8.664197e-02 1.700269e-01 2 28926468 28926554 87 + 2.356 2.358 0.007
ENSG00000163811 E015 375.907255 0.0012187276 5.174223e-02 1.122589e-01 2 28927569 28927700 132 + 2.462 2.456 -0.020
ENSG00000163811 E016 355.299250 0.0017157265 5.879727e-01 7.112142e-01 2 28929579 28929710 132 + 2.431 2.464 0.112
ENSG00000163811 E017 3.921248 0.0041285318 8.344567e-01 8.963734e-01 2 28930058 28930149 92 + 0.607 0.608 0.004
ENSG00000163811 E018 250.835793 0.0002278508 2.400954e-01 3.742603e-01 2 28935521 28935607 87 + 2.269 2.352 0.278
ENSG00000163811 E019 2.912047 0.0054511888 3.085998e-01 4.508762e-01 2 28935608 28935740 133 + 0.468 0.683 0.978
ENSG00000163811 E020 214.208975 0.0002746125 1.690139e-01 2.869128e-01 2 28936922 28936953 32 + 2.200 2.292 0.307
ENSG00000163811 E021 264.710771 0.0001803061 1.420238e-01 2.508086e-01 2 28937931 28937994 64 + 2.292 2.382 0.300
ENSG00000163811 E022 345.297862 0.0001865945 7.211882e-01 8.156346e-01 2 28941461 28941574 114 + 2.414 2.472 0.195
ENSG00000163811 E023 324.500766 0.0005061327 8.543691e-01 9.098933e-01 2 28942312 28942381 70 + 2.389 2.443 0.182
ENSG00000163811 E024 268.308353 0.0009137107 4.341845e-01 5.765743e-01 2 28946450 28946499 50 + 2.302 2.375 0.243
ENSG00000163811 E025 1226.360246 0.0083560410 5.317125e-07 4.679085e-06 2 28946600 28948219 1620 + 2.921 3.160 0.797