ENSG00000163781

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000260810 ENSG00000163781 HEK293_OSMI2_2hA HEK293_TMG_2hB TOPBP1 protein_coding protein_coding 25.46247 8.291585 39.88098 0.6903714 0.6519684 2.264604 8.001312 4.658350 11.049806 0.3005720 0.8758257 1.244342 0.4021625 0.56920000 0.27670000 -0.29250000 0.000555015 0.000555015 FALSE TRUE
ENST00000505804 ENSG00000163781 HEK293_OSMI2_2hA HEK293_TMG_2hB TOPBP1 protein_coding retained_intron 25.46247 8.291585 39.88098 0.6903714 0.6519684 2.264604 2.730187 1.067959 3.325463 0.1878480 0.4696901 1.629585 0.1238417 0.12763333 0.08326667 -0.04436667 0.217081054 0.000555015   FALSE
ENST00000513818 ENSG00000163781 HEK293_OSMI2_2hA HEK293_TMG_2hB TOPBP1 protein_coding retained_intron 25.46247 8.291585 39.88098 0.6903714 0.6519684 2.264604 3.384504 1.071227 5.340310 0.1938809 0.5410774 2.306953 0.1223875 0.12763333 0.13373333 0.00610000 0.936791953 0.000555015 FALSE TRUE
MSTRG.23733.5 ENSG00000163781 HEK293_OSMI2_2hA HEK293_TMG_2hB TOPBP1 protein_coding   25.46247 8.291585 39.88098 0.6903714 0.6519684 2.264604 4.105064 0.389380 8.295633 0.3258076 0.5595528 4.378256 0.1103792 0.04776667 0.20833333 0.16056667 0.119630532 0.000555015 FALSE TRUE
MSTRG.23733.6 ENSG00000163781 HEK293_OSMI2_2hA HEK293_TMG_2hB TOPBP1 protein_coding   25.46247 8.291585 39.88098 0.6903714 0.6519684 2.264604 5.925526 0.778575 10.748328 0.3926258 0.1585684 3.770062 0.1858000 0.08746667 0.26976667 0.18230000 0.371446429 0.000555015 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000163781 E001 2.5098381 0.0061306528 2.514188e-02 6.217193e-02 3 133598175 133598462 288 - 0.380 0.761 1.772
ENSG00000163781 E002 0.6256415 0.0175400243 7.559531e-01 8.411482e-01 3 133598463 133598555 93 - 0.166 0.226 0.551
ENSG00000163781 E003 1.7995922 0.0086030266 1.800381e-01 3.012208e-01 3 133598556 133598630 75 - 0.319 0.572 1.329
ENSG00000163781 E004 5.5867246 0.0038944701 3.462890e-02 8.095190e-02 3 133600238 133600270 33 - 0.660 0.963 1.198
ENSG00000163781 E005 89.2755096 0.0143754246 2.929594e-10 4.775861e-09 3 133600271 133600789 519 - 1.728 2.183 1.531
ENSG00000163781 E006 123.7430637 0.0165242529 5.797907e-11 1.064647e-09 3 133600790 133600987 198 - 1.857 2.336 1.603
ENSG00000163781 E007 64.6381847 0.0006859752 9.959579e-18 4.898267e-16 3 133600988 133601018 31 - 1.605 2.023 1.409
ENSG00000163781 E008 253.7035826 0.0002513725 4.548288e-49 2.603201e-46 3 133601019 133601393 375 - 2.210 2.580 1.235
ENSG00000163781 E009 192.3582856 0.0003045977 1.092746e-23 1.045543e-21 3 133608535 133608696 162 - 2.117 2.415 0.997
ENSG00000163781 E010 183.0304697 0.0002219471 8.351163e-11 1.493536e-09 3 133608873 133608962 90 - 2.126 2.332 0.686
ENSG00000163781 E011 235.5219527 0.0002051665 1.511346e-12 3.596081e-11 3 133611004 133611141 138 - 2.238 2.436 0.661
ENSG00000163781 E012 203.2683488 0.0002391472 6.437959e-08 6.786778e-07 3 133612389 133612552 164 - 2.183 2.350 0.558
ENSG00000163781 E013 75.4923067 0.0005729922 7.593153e-02 1.530058e-01 3 133616814 133616820 7 - 1.776 1.873 0.328
ENSG00000163781 E014 158.0423674 0.0013875938 8.360473e-02 1.651840e-01 3 133616821 133616925 105 - 2.099 2.175 0.254
ENSG00000163781 E015 192.9113724 0.0002183311 3.230017e-01 4.663398e-01 3 133617160 133617326 167 - 2.194 2.236 0.140
ENSG00000163781 E016 12.5651635 0.0015971863 1.007525e-01 1.916064e-01 3 133617327 133617526 200 - 1.000 1.186 0.671
ENSG00000163781 E017 11.0371707 0.0022179733 8.490680e-01 9.063235e-01 3 133618133 133618212 80 - 1.007 0.994 -0.047
ENSG00000163781 E018 211.1845131 0.0001851173 8.284875e-01 8.922139e-01 3 133618213 133618433 221 - 2.240 2.245 0.015
ENSG00000163781 E019 213.6991561 0.0007367453 6.199167e-01 7.372107e-01 3 133620155 133620347 193 - 2.247 2.243 -0.014
ENSG00000163781 E020 167.4513544 0.0002474514 2.934007e-02 7.066258e-02 3 133623091 133623193 103 - 2.155 2.090 -0.217
ENSG00000163781 E021 176.8338398 0.0002476247 7.799267e-01 8.585250e-01 3 133623311 133623457 147 - 2.163 2.165 0.007
ENSG00000163781 E022 134.3519420 0.0007798519 6.024058e-02 1.270168e-01 3 133624052 133624175 124 - 2.059 1.994 -0.219
ENSG00000163781 E023 0.5181333 0.0201582690 5.315827e-01 6.640034e-01 3 133628107 133628361 255 - 0.117 0.226 1.137
ENSG00000163781 E024 122.2274036 0.0004692768 2.663402e-01 4.045165e-01 3 133628362 133628471 110 - 2.013 1.979 -0.115
ENSG00000163781 E025 152.5084452 0.0036701132 4.076884e-01 5.512715e-01 3 133628560 133628733 174 - 2.106 2.080 -0.089
ENSG00000163781 E026 203.3325783 0.0125005598 2.192396e-01 3.494535e-01 3 133637876 133638162 287 - 2.237 2.178 -0.197
ENSG00000163781 E027 187.1774626 0.0040409684 3.449630e-03 1.158091e-02 3 133639959 133640170 212 - 2.213 2.092 -0.407
ENSG00000163781 E028 177.0829886 0.0002407096 9.620050e-03 2.782937e-02 3 133643200 133643372 173 - 2.180 2.102 -0.259
ENSG00000163781 E029 129.1539431 0.0002442477 1.137039e-04 5.847774e-04 3 133644020 133644096 77 - 2.056 1.908 -0.498
ENSG00000163781 E030 82.4924683 0.0003478822 3.256315e-05 1.920465e-04 3 133644097 133644112 16 - 1.873 1.663 -0.708
ENSG00000163781 E031 205.2301167 0.0002834407 2.701420e-07 2.522804e-06 3 133644113 133644363 251 - 2.258 2.098 -0.536
ENSG00000163781 E032 159.2854850 0.0002953193 1.314790e-03 5.026293e-03 3 133649383 133649604 222 - 2.138 2.031 -0.358
ENSG00000163781 E033 72.3554922 0.0003957027 4.473031e-02 9.971081e-02 3 133649605 133649633 29 - 1.797 1.700 -0.327
ENSG00000163781 E034 2.0914649 0.0798552985 4.388884e-02 9.817930e-02 3 133649634 133649779 146 - 0.502 0.001 -10.537
ENSG00000163781 E035 162.5055538 0.0002262492 6.511198e-07 5.628687e-06 3 133649780 133649943 164 - 2.159 1.985 -0.585
ENSG00000163781 E036 123.8339108 0.0003264453 1.396894e-09 2.016438e-08 3 133652463 133652547 85 - 2.055 1.795 -0.874
ENSG00000163781 E037 114.0804532 0.0005961246 5.552118e-12 1.204572e-10 3 133652548 133652614 67 - 2.027 1.700 -1.101
ENSG00000163781 E038 57.6271028 0.0004303878 3.706932e-09 4.942759e-08 3 133652615 133652629 15 - 1.744 1.345 -1.366
ENSG00000163781 E039 170.6841402 0.0037773838 5.848740e-11 1.072629e-09 3 133653345 133653524 180 - 2.201 1.886 -1.055
ENSG00000163781 E040 147.9507881 0.0005831997 2.612073e-12 5.993090e-11 3 133655290 133655486 197 - 2.135 1.849 -0.961
ENSG00000163781 E041 128.4434971 0.0002427897 5.835364e-12 1.260682e-10 3 133656676 133656857 182 - 2.076 1.781 -0.993
ENSG00000163781 E042 124.6231596 0.0002814528 5.790761e-12 1.251903e-10 3 133657798 133657941 144 - 2.063 1.761 -1.017
ENSG00000163781 E043 107.1860458 0.0013073672 4.836968e-07 4.288819e-06 3 133659016 133659150 135 - 1.992 1.750 -0.813
ENSG00000163781 E044 67.8408409 0.0003971477 9.521149e-09 1.179001e-07 3 133661044 133661129 86 - 1.810 1.462 -1.183
ENSG00000163781 E045 23.5078909 0.0015399135 2.998150e-04 1.378885e-03 3 133661130 133661134 5 - 1.363 0.994 -1.313
ENSG00000163781 E046 40.1028941 0.0005380411 9.207071e-06 6.171504e-05 3 133661769 133662184 416 - 1.585 1.240 -1.192
ENSG00000163781 E047 0.0000000       3 133662185 133662380 196 -      
ENSG00000163781 E048 0.0000000       3 133671157 133671206 50 -