ENSG00000163754

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000296048 ENSG00000163754 HEK293_OSMI2_2hA HEK293_TMG_2hB GYG1 protein_coding protein_coding 36.94834 44.80443 35.00492 9.187734 1.558807 -0.3559933 32.414736 36.732902 31.8566395 6.672863 1.3905270 -0.2054187 0.8877208 0.8270000 0.910466667 0.08346667 0.09681987 0.00145419 FALSE TRUE
ENST00000479119 ENSG00000163754 HEK293_OSMI2_2hA HEK293_TMG_2hB GYG1 protein_coding processed_transcript 36.94834 44.80443 35.00492 9.187734 1.558807 -0.3559933 2.730363 6.817993 0.1316283 2.683449 0.1316283 -5.5912805 0.0615500 0.1426667 0.003466667 -0.13920000 0.00145419 0.00145419   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000163754 E001 1.3640887 0.0099934951 1.892857e-01 3.128346e-01 3 148991408 148991451 44 + 0.486 0.284 -1.161
ENSG00000163754 E002 5.0574556 0.0032452331 4.672612e-02 1.033366e-01 3 148991452 148991511 60 + 0.896 0.672 -0.897
ENSG00000163754 E003 12.2012232 0.0014451978 1.260621e-02 3.498334e-02 3 148991512 148991539 28 + 1.226 1.035 -0.684
ENSG00000163754 E004 69.6224610 0.0014123161 1.634434e-02 4.348370e-02 3 148991540 148991557 18 + 1.888 1.822 -0.223
ENSG00000163754 E005 77.1649083 0.0013901794 2.021311e-02 5.193274e-02 3 148991558 148991567 10 + 1.929 1.871 -0.194
ENSG00000163754 E006 78.8234805 0.0017049508 1.849966e-02 4.823033e-02 3 148991568 148991571 4 + 1.939 1.878 -0.206
ENSG00000163754 E007 139.4464297 0.0046720535 4.171611e-04 1.840173e-03 3 148991572 148991647 76 + 2.214 2.090 -0.415
ENSG00000163754 E008 1.7692129 0.0080741317 5.850785e-01 7.088471e-01 3 148992371 148992657 287 + 0.376 0.499 0.646
ENSG00000163754 E009 0.6653823 0.0171383060 2.294684e-01 3.617922e-01 3 148992658 148992772 115 + 0.308 0.117 -1.743
ENSG00000163754 E010 177.7577719 0.0034980425 4.549232e-04 1.986214e-03 3 148994142 148994211 70 + 2.307 2.208 -0.330
ENSG00000163754 E011 173.9007192 0.0004663129 1.318502e-07 1.309153e-06 3 148994212 148994260 49 + 2.303 2.200 -0.343
ENSG00000163754 E012 122.7874892 0.0065469040 5.435819e-03 1.711398e-02 3 148994261 148994277 17 + 2.151 2.044 -0.356
ENSG00000163754 E013 108.0573784 0.0016058801 6.131251e-04 2.581273e-03 3 148996302 148996314 13 + 2.090 2.000 -0.303
ENSG00000163754 E014 188.0963664 0.0006005950 1.572861e-03 5.870481e-03 3 148996315 148996427 113 + 2.306 2.262 -0.146
ENSG00000163754 E015 120.7912022 0.0003239081 9.007333e-01 9.406852e-01 3 148996428 148996476 49 + 2.063 2.110 0.159
ENSG00000163754 E016 190.1152666 0.0005694819 4.285208e-02 9.627159e-02 3 148996742 148996830 89 + 2.293 2.280 -0.043
ENSG00000163754 E017 186.2296329 0.0001955899 2.790306e-01 4.187348e-01 3 148996831 148996892 62 + 2.269 2.285 0.055
ENSG00000163754 E018 135.0234425 0.0002824254 2.393391e-01 3.733517e-01 3 148996893 148996904 12 + 2.135 2.144 0.030
ENSG00000163754 E019 0.0000000       3 149000934 149001183 250 +      
ENSG00000163754 E020 0.1472490 0.0440914964 4.194375e-01   3 149001446 149001593 148 + 0.128 0.000 -10.999
ENSG00000163754 E021 0.5149242 0.2613024395 7.397170e-01 8.293598e-01 3 149003953 149003953 1 + 0.129 0.209 0.846
ENSG00000163754 E022 0.6600969 0.1137647592 8.521530e-01 9.083663e-01 3 149003954 149003998 45 + 0.227 0.209 -0.156
ENSG00000163754 E023 0.4375944 0.0239679960 6.061402e-02 1.276643e-01 3 149003999 149004050 52 + 0.308 0.000 -12.832
ENSG00000163754 E024 0.1482932 0.0411597534 5.725526e-01   3 149007999 149008103 105 + 0.000 0.116 10.048
ENSG00000163754 E025 0.3686942 0.0311011143 8.935303e-01 9.359177e-01 3 149008252 149008276 25 + 0.128 0.117 -0.156
ENSG00000163754 E026 0.6579068 0.4684587608 9.079602e-01 9.455742e-01 3 149008881 149009275 395 + 0.227 0.210 -0.146
ENSG00000163754 E027 318.3924456 0.0006783282 4.279897e-01 5.707977e-01 3 149009276 149009402 127 + 2.494 2.520 0.085
ENSG00000163754 E028 441.4304642 0.0003690961 2.495877e-02 6.178688e-02 3 149024053 149024272 220 + 2.602 2.685 0.278
ENSG00000163754 E029 2.2175051 0.0070913188 1.473682e-01 2.581195e-01 3 149026452 149026502 51 + 0.612 0.405 -1.008
ENSG00000163754 E030 1119.8628725 0.0038555617 1.432372e-10 2.462643e-09 3 149026760 149027632 873 + 2.930 3.117 0.621
ENSG00000163754 E031 21.9788741 0.0190891614 5.133971e-04 2.209686e-03 3 149027633 149032358 4726 + 1.509 1.191 -1.108