ENSG00000163743

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000324439 ENSG00000163743 HEK293_OSMI2_2hA HEK293_TMG_2hB RCHY1 protein_coding protein_coding 24.59083 10.99717 34.0535 1.825736 2.069108 1.629783 3.2185130 1.0194185 6.2268645 0.3329910 0.3941518 2.5989913 0.10947500 0.08826667 0.182833333 0.09456667 0.0008890196 0.0008890196 FALSE TRUE
ENST00000380840 ENSG00000163743 HEK293_OSMI2_2hA HEK293_TMG_2hB RCHY1 protein_coding protein_coding 24.59083 10.99717 34.0535 1.825736 2.069108 1.629783 0.6420368 1.4913863 0.1624564 0.2420402 0.1624564 -3.1219914 0.04830417 0.13616667 0.005433333 -0.13073333 0.0066129428 0.0008890196 FALSE TRUE
ENST00000505105 ENSG00000163743 HEK293_OSMI2_2hA HEK293_TMG_2hB RCHY1 protein_coding nonsense_mediated_decay 24.59083 10.99717 34.0535 1.825736 2.069108 1.629783 6.6469096 4.4759368 8.6951152 0.5139282 0.2279157 0.9564541 0.30850417 0.41346667 0.258200000 -0.15526667 0.0056042235 0.0008890196 TRUE TRUE
ENST00000512567 ENSG00000163743 HEK293_OSMI2_2hA HEK293_TMG_2hB RCHY1 protein_coding retained_intron 24.59083 10.99717 34.0535 1.825736 2.069108 1.629783 2.6325580 0.9384872 1.2588690 0.4716984 1.2588690 0.4198429 0.10868750 0.08110000 0.034433333 -0.04666667 0.4520513446 0.0008890196 FALSE TRUE
ENST00000512706 ENSG00000163743 HEK293_OSMI2_2hA HEK293_TMG_2hB RCHY1 protein_coding protein_coding 24.59083 10.99717 34.0535 1.825736 2.069108 1.629783 6.8973474 1.7164764 10.9748986 0.1872123 1.7082720 2.6696190 0.25958750 0.16146667 0.318400000 0.15693333 0.0126288207 0.0008890196 FALSE TRUE
ENST00000513257 ENSG00000163743 HEK293_OSMI2_2hA HEK293_TMG_2hB RCHY1 protein_coding protein_coding 24.59083 10.99717 34.0535 1.825736 2.069108 1.629783 1.8107412 0.4777979 2.5873683 0.1174777 0.5530534 2.4126950 0.06509167 0.04250000 0.075400000 0.03290000 0.2407493185 0.0008890196 FALSE TRUE
MSTRG.25026.5 ENSG00000163743 HEK293_OSMI2_2hA HEK293_TMG_2hB RCHY1 protein_coding   24.59083 10.99717 34.0535 1.825736 2.069108 1.629783 1.4664434 0.6263169 2.7044189 0.3137353 1.1619554 2.0928260 0.05349167 0.05573333 0.083266667 0.02753333 0.8750431729 0.0008890196 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000163743 E001 205.5491319 0.0072248326 3.519948e-08 3.908126e-07 4 75479033 75481468 2436 - 2.356 2.086 -0.902
ENSG00000163743 E002 57.7100407 0.0004352757 2.676232e-01 4.059468e-01 4 75481469 75482007 539 - 1.760 1.698 -0.208
ENSG00000163743 E003 53.6120230 0.0015751869 1.330132e-08 1.602069e-07 4 75482008 75482085 78 - 1.602 1.887 0.964
ENSG00000163743 E004 168.9893668 0.0017229270 1.867746e-13 5.114017e-12 4 75482086 75482341 256 - 2.116 2.349 0.780
ENSG00000163743 E005 118.0531744 0.0003938409 1.909378e-05 1.190005e-04 4 75482342 75482412 71 - 1.999 2.139 0.470
ENSG00000163743 E006 169.3844965 0.0002185939 5.380401e-03 1.696148e-02 4 75482413 75482537 125 - 2.182 2.256 0.247
ENSG00000163743 E007 134.0191958 0.0002699897 7.948919e-02 1.587228e-01 4 75482538 75482603 66 - 2.089 2.141 0.173
ENSG00000163743 E008 124.5262994 0.0003554904 4.614433e-02 1.023019e-01 4 75482604 75482666 63 - 2.054 2.117 0.210
ENSG00000163743 E009 95.0040958 0.0003274043 2.802688e-01 4.201172e-01 4 75490581 75490597 17 - 1.946 1.983 0.125
ENSG00000163743 E010 151.2300180 0.0003154280 7.181949e-01 8.133904e-01 4 75490598 75490663 66 - 2.162 2.146 -0.055
ENSG00000163743 E011 140.0144221 0.0002978826 1.394075e-01 2.472299e-01 4 75490664 75490701 38 - 2.139 2.084 -0.181
ENSG00000163743 E012 7.7043785 0.0145468333 6.075205e-01 7.274837e-01 4 75490702 75491582 881 - 0.937 0.863 -0.279
ENSG00000163743 E013 1.1844606 0.0108785241 8.720986e-01 9.217560e-01 4 75491583 75491606 24 - 0.344 0.312 -0.199
ENSG00000163743 E014 1.1801942 0.0117751968 8.710446e-01 9.210871e-01 4 75491607 75491610 4 - 0.344 0.312 -0.199
ENSG00000163743 E015 120.3542116 0.0002480238 4.533922e-02 1.008131e-01 4 75491611 75491637 27 - 2.080 2.004 -0.255
ENSG00000163743 E016 10.5259827 0.0021169060 6.559915e-01 7.657532e-01 4 75491638 75491723 86 - 1.052 0.998 -0.198
ENSG00000163743 E017 154.7063765 0.0002333848 2.035522e-03 7.336965e-03 4 75491724 75491782 59 - 2.196 2.094 -0.343
ENSG00000163743 E018 106.9598835 0.0002801301 3.409218e-03 1.146454e-02 4 75491889 75491910 22 - 2.041 1.925 -0.391
ENSG00000163743 E019 105.2855581 0.0002935448 8.918609e-02 1.738902e-01 4 75491911 75491933 23 - 2.021 1.951 -0.235
ENSG00000163743 E020 142.9891274 0.0002876419 1.501054e-01 2.618148e-01 4 75494101 75494179 79 - 2.148 2.096 -0.176
ENSG00000163743 E021 0.1451727 0.0444177007 1.000000e+00   4 75494180 75494431 252 - 0.080 0.000 -7.911
ENSG00000163743 E022 70.8857115 0.0005293775 8.097384e-01 8.792286e-01 4 75508820 75508823 4 - 1.835 1.818 -0.058
ENSG00000163743 E023 146.1737671 0.0002386758 4.668529e-01 6.063864e-01 4 75508824 75508935 112 - 2.149 2.121 -0.096
ENSG00000163743 E024 123.4348749 0.0003138984 3.161002e-01 4.588654e-01 4 75509177 75509296 120 - 2.078 2.037 -0.138
ENSG00000163743 E025 3.0180583 0.1222800286 7.634734e-01 8.467757e-01 4 75509297 75509807 511 - 0.605 0.495 -0.510
ENSG00000163743 E026 0.5181333 0.0212177651 8.107968e-01 8.799692e-01 4 75514155 75514196 42 - 0.147 0.184 0.390
ENSG00000163743 E027 67.6473553 0.0006880300 8.229719e-01 8.884040e-01 4 75514197 75514240 44 - 1.806 1.811 0.016
ENSG00000163743 E028 57.4915796 0.0064908456 8.428735e-01 9.021144e-01 4 75514241 75514430 190 - 1.736 1.737 0.002
ENSG00000163743 E029 2.6957864 0.0066890442 2.871226e-01 4.275997e-01 4 75514663 75514984 322 - 0.604 0.411 -0.935