ENSG00000163577

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000295822 ENSG00000163577 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF5A2 protein_coding protein_coding 7.118512 3.718324 13.2799 0.47304 0.9720449 1.833731 1.7798182 0.8210424 3.461277 0.12854451 0.07303538 2.0624725 0.24394583 0.23133333 0.26416667 0.03283333 0.82215396 0.01049395 FALSE TRUE
ENST00000460117 ENSG00000163577 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF5A2 protein_coding processed_transcript 7.118512 3.718324 13.2799 0.47304 0.9720449 1.833731 0.8222981 0.1216798 1.919513 0.12167984 0.36881290 3.8731304 0.08480000 0.02613333 0.14516667 0.11903333 0.10594514 0.01049395   FALSE
ENST00000474096 ENSG00000163577 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF5A2 protein_coding protein_coding 7.118512 3.718324 13.2799 0.47304 0.9720449 1.833731 1.5552714 0.5399273 3.552036 0.53992734 0.42019414 2.6953892 0.27742500 0.11596667 0.26860000 0.15263333 0.22773454 0.01049395 FALSE TRUE
ENST00000474417 ENSG00000163577 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF5A2 protein_coding protein_coding 7.118512 3.718324 13.2799 0.47304 0.9720449 1.833731 2.2306906 2.0868209 3.318322 0.09817069 0.77839659 0.6665913 0.29796667 0.57923333 0.24670000 -0.33253333 0.01049395 0.01049395 FALSE FALSE
ENST00000487522 ENSG00000163577 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF5A2 protein_coding protein_coding 7.118512 3.718324 13.2799 0.47304 0.9720449 1.833731 0.7304337 0.1488535 1.028750 0.14885354 0.29871002 2.7090793 0.09586667 0.04736667 0.07536667 0.02800000 0.43581830 0.01049395 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000163577 E001 222.48871 0.0010606528 0.004118133 0.013473613 3 170888418 170893135 4718 - 2.325 2.251 -0.246
ENSG00000163577 E002 15.78555 0.0190646274 0.934289279 0.962570868 3 170893136 170893215 80 - 1.182 1.185 0.008
ENSG00000163577 E003 28.17920 0.0011921698 0.333925418 0.477687935 3 170893216 170893348 133 - 1.444 1.368 -0.262
ENSG00000163577 E004 37.06218 0.0013350554 0.377590662 0.521913164 3 170893349 170893419 71 - 1.558 1.496 -0.210
ENSG00000163577 E005 56.87302 0.0005271583 0.216429189 0.346121176 3 170894292 170894383 92 - 1.705 1.761 0.190
ENSG00000163577 E006 44.90081 0.0005772223 0.019704194 0.050861111 3 170894384 170894423 40 - 1.583 1.706 0.417
ENSG00000163577 E007 54.41573 0.0005396062 0.002155583 0.007706817 3 170906989 170907093 105 - 1.657 1.802 0.494
ENSG00000163577 E008 56.09292 0.0009107928 0.498325540 0.634803589 3 170907642 170907799 158 - 1.705 1.735 0.100
ENSG00000163577 E009 28.80843 0.0116975404 0.393442695 0.537339693 3 170907800 170907841 42 - 1.456 1.376 -0.276
ENSG00000163577 E010 24.28267 0.0014103612 0.953428068 0.974863642 3 170908543 170908644 102 - 1.358 1.358 -0.002