ENSG00000163558

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000295797 ENSG00000163558 HEK293_OSMI2_2hA HEK293_TMG_2hB PRKCI protein_coding protein_coding 12.46487 3.67377 21.99082 0.5509728 0.3028841 2.578302 11.4804294 2.009827 20.81815 0.08090273 0.6476125 3.366231 0.8387667 0.5662667 0.9462 0.3799333 2.173914e-06 3.356673e-24 FALSE TRUE
ENST00000485837 ENSG00000163558 HEK293_OSMI2_2hA HEK293_TMG_2hB PRKCI protein_coding retained_intron 12.46487 3.67377 21.99082 0.5509728 0.3028841 2.578302 0.4078796 1.663943 0.00000 0.50015823 0.0000000 -7.387107 0.1099583 0.4337333 0.0000 -0.4337333 3.356673e-24 3.356673e-24 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000163558 E001 65.9356408 0.0005025271 1.111584e-05 7.308468e-05 3 170222424 170222770 347 + 1.641 1.441 -0.685
ENSG00000163558 E002 85.9720003 0.0003114985 4.273917e-07 3.832446e-06 3 170235230 170235351 122 + 1.755 1.554 -0.685
ENSG00000163558 E003 85.7592864 0.0011423263 1.834851e-07 1.771336e-06 3 170259969 170260058 90 + 1.756 1.528 -0.775
ENSG00000163558 E004 71.5243473 0.0021790471 1.208930e-02 3.374796e-02 3 170263379 170263429 51 + 1.664 1.622 -0.142
ENSG00000163558 E005 94.2835754 0.0003111821 4.346447e-02 9.740365e-02 3 170267915 170268000 86 + 1.773 1.795 0.074
ENSG00000163558 E006 128.8872151 0.0121947699 1.611235e-01 2.765789e-01 3 170270421 170270561 141 + 1.906 1.933 0.089
ENSG00000163558 E007 82.3941606 0.0025032756 3.018979e-02 7.234026e-02 3 170273286 170273340 55 + 1.719 1.709 -0.033
ENSG00000163558 E008 84.3111316 0.0054091206 1.802724e-02 4.719026e-02 3 170275229 170275287 59 + 1.731 1.692 -0.132
ENSG00000163558 E009 0.3268771 0.0317642060 1.516013e-01   3 170280145 170280226 82 + 0.043 0.306 3.305
ENSG00000163558 E010 69.8055157 0.0004060203 4.037996e-05 2.324833e-04 3 170280227 170280239 13 + 1.663 1.501 -0.554
ENSG00000163558 E011 147.6310537 0.0006572893 3.627294e-05 2.112060e-04 3 170280240 170280403 164 + 1.975 1.913 -0.211
ENSG00000163558 E012 110.4151936 0.0164955600 2.779659e-02 6.761226e-02 3 170281166 170281263 98 + 1.851 1.803 -0.161
ENSG00000163558 E013 1.3318114 0.0096279870 2.785457e-01 4.182176e-01 3 170281264 170281537 274 + 0.286 0.000 -10.379
ENSG00000163558 E014 1.7757486 0.0076800777 1.630169e-01 2.790731e-01 3 170281610 170281881 272 + 0.351 0.000 -10.794
ENSG00000163558 E015 97.2453323 0.0116013927 8.401461e-02 1.658260e-01 3 170281882 170281968 87 + 1.792 1.803 0.037
ENSG00000163558 E016 109.9023443 0.0047781788 9.969049e-02 1.900129e-01 3 170284461 170284596 136 + 1.841 1.881 0.136
ENSG00000163558 E017 1.0373134 0.0117265282 4.152205e-01 5.586175e-01 3 170291642 170291853 212 + 0.237 0.000 -10.017
ENSG00000163558 E018 88.3502815 0.0003365282 8.526426e-04 3.447089e-03 3 170291854 170291941 88 + 1.758 1.691 -0.226
ENSG00000163558 E019 0.2214452 0.0433988811 4.541540e-02   3 170293332 170293346 15 + 0.000 0.307 13.546
ENSG00000163558 E020 0.6631923 0.5865260540 4.303939e-01 5.730373e-01 3 170293347 170293382 36 + 0.117 0.314 1.774
ENSG00000163558 E021 101.1582475 0.0002917610 7.177150e-04 2.962480e-03 3 170293383 170293508 126 + 1.813 1.758 -0.186
ENSG00000163558 E022 88.7611397 0.0003004683 9.279751e-03 2.698421e-02 3 170295911 170295990 80 + 1.752 1.734 -0.061
ENSG00000163558 E023 96.6907569 0.0003230597 3.293431e-02 7.767286e-02 3 170297304 170297393 90 + 1.785 1.802 0.057
ENSG00000163558 E024 109.1593472 0.0003959814 2.621350e-03 9.131323e-03 3 170298995 170299110 116 + 1.842 1.816 -0.087
ENSG00000163558 E025 763.7142064 0.0075547156 8.280813e-20 5.140155e-18 3 170303040 170305977 2938 + 2.611 3.020 1.358