ENSG00000163535

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000357799 ENSG00000163535 HEK293_OSMI2_2hA HEK293_TMG_2hB SGO2 protein_coding protein_coding 7.620953 1.58602 11.80422 0.08153843 0.540525 2.887973 2.8623618 1.03096254 4.9574753 0.09006260 1.2480562 2.2545946 0.4829042 0.64783333 0.4198333 -0.2280 2.606535e-01 5.734375e-06 FALSE TRUE
ENST00000469840 ENSG00000163535 HEK293_OSMI2_2hA HEK293_TMG_2hB SGO2 protein_coding processed_transcript 7.620953 1.58602 11.80422 0.08153843 0.540525 2.887973 0.3457046 0.46785973 0.5211919 0.09675733 0.1080673 0.1526461 0.1117458 0.30230000 0.0435000 -0.2588 5.734375e-06 5.734375e-06 FALSE FALSE
MSTRG.19736.2 ENSG00000163535 HEK293_OSMI2_2hA HEK293_TMG_2hB SGO2 protein_coding   7.620953 1.58602 11.80422 0.08153843 0.540525 2.887973 4.1859654 0.08719802 5.9526191 0.08719802 1.3241378 5.9388755 0.3846000 0.04986667 0.5038667 0.4540 1.785684e-02 5.734375e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000163535 E001 0.0000000       2 200510008 200510241 234 +      
ENSG00000163535 E002 0.0000000       2 200510242 200510266 25 +      
ENSG00000163535 E003 0.0000000       2 200525749 200525836 88 +      
ENSG00000163535 E004 0.7385489 0.0345713270 8.179717e-01 8.849072e-01 2 200526136 200526158 23 + 0.189 0.000 -9.704
ENSG00000163535 E005 1.8478684 0.0075704439 7.947328e-01 8.688769e-01 2 200526159 200526166 8 + 0.342 0.295 -0.299
ENSG00000163535 E006 6.7503189 0.0052326248 2.897474e-02 6.995703e-02 2 200526167 200526206 40 + 0.763 0.295 -2.300
ENSG00000163535 E007 10.6006942 0.0065495564 2.037182e-03 7.342110e-03 2 200526207 200526252 46 + 0.936 0.295 -2.970
ENSG00000163535 E008 0.2903454 0.2961056722 1.000000e+00   2 200531775 200531901 127 + 0.085 0.000 -9.127
ENSG00000163535 E009 0.4460135 0.0404959144 1.000000e+00 1.000000e+00 2 200532303 200532355 53 + 0.123 0.000 -10.236
ENSG00000163535 E010 16.5625495 0.0010865745 5.050442e-02 1.100585e-01 2 200532974 200533108 135 + 1.091 0.835 -0.956
ENSG00000163535 E011 0.1472490 0.0444773532 6.734708e-01   2 200533109 200533375 267 + 0.045 0.000 -8.661
ENSG00000163535 E012 14.5120932 0.0204205622 4.982583e-01 6.347363e-01 2 200534996 200535045 50 + 1.024 0.945 -0.292
ENSG00000163535 E013 21.4397734 0.0009115972 7.372140e-01 8.274520e-01 2 200535046 200535140 95 + 1.174 1.165 -0.033
ENSG00000163535 E014 18.8211794 0.0010009410 3.042138e-01 4.462265e-01 2 200535141 200535171 31 + 1.131 1.031 -0.364
ENSG00000163535 E015 31.5311650 0.0006831756 7.356483e-02 1.492321e-01 2 200536065 200536142 78 + 1.347 1.193 -0.541
ENSG00000163535 E016 39.8326919 0.0005229442 6.595648e-03 2.017512e-02 2 200542579 200542664 86 + 1.451 1.219 -0.806
ENSG00000163535 E017 0.2966881 0.0268978007 1.000000e+00   2 200542665 200542950 286 + 0.086 0.000 -9.783
ENSG00000163535 E018 4.2176435 0.1121509917 1.353125e-02 3.715205e-02 2 200543025 200543622 598 + 0.471 1.016 2.258
ENSG00000163535 E019 52.2801932 0.0004931077 1.990702e-03 7.198010e-03 2 200569663 200569892 230 + 1.562 1.331 -0.797
ENSG00000163535 E020 0.2966881 0.0268978007 1.000000e+00   2 200569893 200570142 250 + 0.086 0.000 -9.783
ENSG00000163535 E021 523.9486209 0.0001945000 2.531857e-08 2.893303e-07 2 200571050 200573977 2928 + 2.538 2.477 -0.202
ENSG00000163535 E022 61.3674278 0.0004393962 5.375236e-09 6.973378e-08 2 200575311 200575461 151 + 1.548 1.902 1.200
ENSG00000163535 E023 44.4425388 0.0146225475 6.151396e-21 4.402582e-19 2 200583449 200584096 648 + 1.283 2.033 2.554