ENSG00000163527

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000295770 ENSG00000163527 HEK293_OSMI2_2hA HEK293_TMG_2hB STT3B protein_coding protein_coding 56.83592 19.61177 92.7315 2.724587 2.284783 2.24076 46.377135 9.303021 80.9143136 0.5253835 2.298230 3.119252 0.7070333 0.4854667 0.872433333 0.3869667 7.499909e-16 7.499909e-16 FALSE TRUE
ENST00000488151 ENSG00000163527 HEK293_OSMI2_2hA HEK293_TMG_2hB STT3B protein_coding retained_intron 56.83592 19.61177 92.7315 2.724587 2.284783 2.24076 3.889901 8.673462 0.4522843 1.9125502 0.280798 -4.231418 0.1839333 0.4332667 0.004733333 -0.4285333 9.643694e-06 7.499909e-16 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000163527 E001 0.5975289 0.0281870422 7.091913e-01 8.066364e-01 3 31530958 31531988 1031 + 0.176 0.000 -9.691
ENSG00000163527 E002 1.1876829 0.0107638304 9.130591e-01 9.489096e-01 3 31532510 31532568 59 + 0.272 0.267 -0.035
ENSG00000163527 E003 25.8872857 0.0060016596 2.083673e-02 5.324327e-02 3 31532569 31532637 69 + 1.315 1.111 -0.721
ENSG00000163527 E004 78.4359447 0.0227076752 2.265409e-02 5.703321e-02 3 31532638 31532848 211 + 1.779 1.611 -0.568
ENSG00000163527 E005 46.7314442 0.0140383874 1.812546e-02 4.739825e-02 3 31532849 31532920 72 + 1.560 1.379 -0.621
ENSG00000163527 E006 30.3922469 0.0066035482 4.353073e-04 1.910212e-03 3 31532921 31532924 4 + 1.394 1.050 -1.218
ENSG00000163527 E007 66.9799030 0.0114102133 1.871987e-05 1.168739e-04 3 31532925 31532971 47 + 1.725 1.381 -1.176
ENSG00000163527 E008 304.4859975 0.0271970109 2.675224e-07 2.500929e-06 3 31532972 31533312 341 + 2.382 1.964 -1.397
ENSG00000163527 E009 0.1515154 0.0433984490 1.000000e+00   3 31533625 31533686 62 + 0.051 0.000 -7.669
ENSG00000163527 E010 270.6535252 0.0049024882 1.724433e-08 2.035063e-07 3 31576396 31576504 109 + 2.316 2.090 -0.755
ENSG00000163527 E011 472.6657636 0.0001638704 1.646439e-09 2.344682e-08 3 31579809 31580096 288 + 2.542 2.455 -0.290
ENSG00000163527 E012 147.5501120 0.0013055948 1.525462e-02 4.106207e-02 3 31596798 31596805 8 + 2.035 1.984 -0.172
ENSG00000163527 E013 216.0167953 0.0020233432 1.178023e-03 4.566854e-03 3 31596806 31596863 58 + 2.202 2.126 -0.256
ENSG00000163527 E014 264.2316605 0.0001645209 1.268590e-05 8.231567e-05 3 31600360 31600459 100 + 2.290 2.209 -0.270
ENSG00000163527 E015 274.5900456 0.0001746446 7.794988e-05 4.183510e-04 3 31615105 31615203 99 + 2.304 2.240 -0.214
ENSG00000163527 E016 303.0410995 0.0001376995 1.459014e-04 7.294955e-04 3 31616929 31617075 147 + 2.346 2.294 -0.174
ENSG00000163527 E017 234.0052446 0.0002592188 8.498399e-05 4.515865e-04 3 31617940 31617988 49 + 2.238 2.163 -0.250
ENSG00000163527 E018 326.6192201 0.0002198212 2.200819e-04 1.049827e-03 3 31619676 31619785 110 + 2.378 2.331 -0.154
ENSG00000163527 E019 258.2637621 0.0003376706 5.275514e-05 2.955233e-04 3 31619786 31619830 45 + 2.279 2.204 -0.250
ENSG00000163527 E020 3.1828003 0.0048549135 5.065975e-01 6.422502e-01 3 31619831 31619964 134 + 0.527 0.432 -0.474
ENSG00000163527 E021 9.7084917 0.0017887346 2.343781e-02 5.865629e-02 3 31619965 31620296 332 + 0.836 1.165 1.219
ENSG00000163527 E022 576.0099212 0.0001072825 5.899499e-06 4.132223e-05 3 31622097 31622308 212 + 2.621 2.585 -0.120
ENSG00000163527 E023 237.0920943 0.0001900373 4.195238e-02 9.463864e-02 3 31623674 31623677 4 + 2.232 2.225 -0.024
ENSG00000163527 E024 555.5330121 0.0002076490 2.563862e-02 6.319983e-02 3 31623678 31623861 184 + 2.598 2.609 0.037
ENSG00000163527 E025 522.4189428 0.0001059103 3.175450e-01 4.604407e-01 3 31624914 31625085 172 + 2.568 2.607 0.128
ENSG00000163527 E026 438.7883045 0.0001625055 8.293359e-01 8.928370e-01 3 31625954 31626060 107 + 2.489 2.554 0.216
ENSG00000163527 E027 325.3999158 0.0005714273 1.440663e-01 2.535387e-01 3 31626061 31626127 67 + 2.353 2.453 0.334
ENSG00000163527 E028 1.8038612 0.0074878304 5.325948e-01 6.649301e-01 3 31629251 31629297 47 + 0.375 0.267 -0.686
ENSG00000163527 E029 433.7615066 0.0002007710 1.418552e-01 2.505739e-01 3 31629298 31629411 114 + 2.479 2.572 0.312
ENSG00000163527 E030 645.1505602 0.0001438457 2.007644e-02 5.164816e-02 3 31632935 31633147 213 + 2.649 2.753 0.344
ENSG00000163527 E031 9.5697675 0.0018276854 7.853064e-01 8.623777e-01 3 31633148 31633184 37 + 0.888 0.978 0.340
ENSG00000163527 E032 2178.7798955 0.0061872061 5.437314e-12 1.181288e-10 3 31635984 31637616 1633 + 3.142 3.406 0.879