ENSG00000163376

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000417314 ENSG00000163376 HEK293_OSMI2_2hA HEK293_TMG_2hB KBTBD8 protein_coding protein_coding 4.319875 0.8420911 8.701695 0.2059466 0.3834857 3.353874 3.0132517 0.3018533 5.4371513 0.03867251 0.18589333 4.126563 0.5866667 0.4216333 0.62540000 0.20376667 2.751412e-01 2.110787e-09 FALSE TRUE
ENST00000460576 ENSG00000163376 HEK293_OSMI2_2hA HEK293_TMG_2hB KBTBD8 protein_coding protein_coding 4.319875 0.8420911 8.701695 0.2059466 0.3834857 3.353874 0.2967909 0.5402378 0.1273668 0.24329287 0.02467329 -2.002022 0.2706792 0.5783667 0.01446667 -0.56390000 2.110787e-09 2.110787e-09 FALSE FALSE
ENST00000484414 ENSG00000163376 HEK293_OSMI2_2hA HEK293_TMG_2hB KBTBD8 protein_coding protein_coding 4.319875 0.8420911 8.701695 0.2059466 0.3834857 3.353874 0.8790917 0.0000000 2.4197635 0.00000000 0.16561526 7.924672 0.1177875 0.0000000 0.27766667 0.27766667 1.105620e-08 2.110787e-09 FALSE FALSE
MSTRG.23206.1 ENSG00000163376 HEK293_OSMI2_2hA HEK293_TMG_2hB KBTBD8 protein_coding   4.319875 0.8420911 8.701695 0.2059466 0.3834857 3.353874 0.1014704 0.0000000 0.6005946 0.00000000 0.06378832 5.932143 0.0213875 0.0000000 0.06866667 0.06866667 1.854485e-02 2.110787e-09 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000163376 E001 1.3318114 0.0096902261 3.719296e-01 5.164018e-01 3 66998291 66998306 16 + 0.296 0.001 -9.180
ENSG00000163376 E002 13.5482686 0.0207742321 1.606729e-02 4.288572e-02 3 66998307 66998371 65 + 1.024 0.594 -1.710
ENSG00000163376 E003 0.4439371 0.0217305627 1.000000e+00 1.000000e+00 3 66998925 66998980 56 + 0.122 0.000 -8.669
ENSG00000163376 E004 20.8403541 0.0102911243 3.447627e-03 1.157500e-02 3 66998981 66999191 211 + 1.195 0.769 -1.589
ENSG00000163376 E005 39.1363294 0.0005446441 1.124363e-04 5.790755e-04 3 67003195 67003537 343 + 1.451 1.070 -1.342
ENSG00000163376 E006 25.2705227 0.0008482105 1.377576e-04 6.937571e-04 3 67003538 67003697 160 + 1.276 0.769 -1.874
ENSG00000163376 E007 72.4767560 0.0004437278 2.858941e-06 2.149584e-05 3 67003698 67004309 612 + 1.707 1.388 -1.090
ENSG00000163376 E008 269.2545279 0.0014852062 1.269366e-19 7.752340e-18 3 67007922 67011210 3289 + 2.219 2.406 0.625