ENSG00000163322

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000321945 ENSG00000163322 HEK293_OSMI2_2hA HEK293_TMG_2hB ABRAXAS1 protein_coding protein_coding 5.180415 3.826822 7.812709 0.5024064 1.367478 1.027756 0.360689 0.1540684 0.6194959 0.03913114 0.02995705 1.9398995 0.0699375 0.03986667 0.08596667 0.04610000 0.30456991 0.02115407 FALSE TRUE
ENST00000475656 ENSG00000163322 HEK293_OSMI2_2hA HEK293_TMG_2hB ABRAXAS1 protein_coding nonsense_mediated_decay 5.180415 3.826822 7.812709 0.5024064 1.367478 1.027756 1.416137 0.4936957 2.5183320 0.06418642 0.13623078 2.3275615 0.2438083 0.13330000 0.34183333 0.20853333 0.02115407 0.02115407 FALSE TRUE
ENST00000511801 ENSG00000163322 HEK293_OSMI2_2hA HEK293_TMG_2hB ABRAXAS1 protein_coding nonsense_mediated_decay 5.180415 3.826822 7.812709 0.5024064 1.367478 1.027756 1.254488 0.8747572 1.3963646 0.20630765 0.13876192 0.6686171 0.2435417 0.22186667 0.19723333 -0.02463333 0.85758133 0.02115407 FALSE FALSE
MSTRG.25050.10 ENSG00000163322 HEK293_OSMI2_2hA HEK293_TMG_2hB ABRAXAS1 protein_coding   5.180415 3.826822 7.812709 0.5024064 1.367478 1.027756 1.765877 2.1453994 2.5755294 0.30200645 1.25144054 0.2625045 0.3589500 0.56126667 0.29073333 -0.27053333 0.22440092 0.02115407   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000163322 E001 9.2636362 0.0023589757 2.656440e-01 4.037115e-01 4 83459517 83460944 1428 - 0.920 1.064 0.532
ENSG00000163322 E002 46.7099039 0.0008077631 2.278837e-01 3.599066e-01 4 83460945 83462008 1064 - 1.596 1.678 0.280
ENSG00000163322 E003 6.1657073 0.0027193327 9.624364e-01 9.803094e-01 4 83462009 83462074 66 - 0.797 0.820 0.087
ENSG00000163322 E004 53.1437845 0.0004451015 1.646557e-05 1.041103e-04 4 83462075 83462902 828 - 1.716 1.490 -0.772
ENSG00000163322 E005 17.5359677 0.0011846095 2.152093e-03 7.696081e-03 4 83463494 83463608 115 - 1.264 0.959 -1.100
ENSG00000163322 E006 0.1817044 0.0434608826 1.259001e-01   4 83463723 83463934 212 - 0.000 0.210 11.793
ENSG00000163322 E007 0.1451727 0.0444781688 1.000000e+00   4 83466839 83466945 107 - 0.068 0.000 -9.787
ENSG00000163322 E008 15.1595447 0.0013508828 1.448505e-03 5.468392e-03 4 83467454 83467538 85 - 1.208 0.859 -1.282
ENSG00000163322 E009 16.6290281 0.0011932009 8.273875e-05 4.409669e-04 4 83469032 83469151 120 - 1.256 0.820 -1.602
ENSG00000163322 E010 1.4917459 0.0188606284 5.202105e-02 1.127558e-01 4 83469152 83469901 750 - 0.432 0.000 -12.784
ENSG00000163322 E011 11.8304126 0.0015090514 1.661787e-02 4.409534e-02 4 83470203 83470273 71 - 1.099 0.820 -1.045
ENSG00000163322 E012 13.9404633 0.0014485633 5.488941e-02 1.178035e-01 4 83470274 83470396 123 - 1.154 0.959 -0.713
ENSG00000163322 E013 8.7594014 0.0019138706 1.805786e-02 4.725606e-02 4 83472222 83472288 67 - 0.993 0.675 -1.244
ENSG00000163322 E014 9.5106464 0.0020830809 1.276340e-01 2.308562e-01 4 83476643 83476679 37 - 1.001 0.820 -0.686
ENSG00000163322 E015 2.7969441 0.0059480364 8.486892e-06 5.731352e-05 4 83478808 83480368 1561 - 0.225 0.928 3.458
ENSG00000163322 E016 5.8551056 0.0031908166 3.867841e-05 2.237082e-04 4 83481064 83481124 61 - 0.571 1.108 2.120
ENSG00000163322 E017 22.7908587 0.0010112702 5.532423e-02 1.185527e-01 4 83482154 83482244 91 - 1.272 1.434 0.563
ENSG00000163322 E018 11.3208647 0.0260963755 3.112857e-07 2.870109e-06 4 83483992 83484166 175 - 0.773 1.403 2.301
ENSG00000163322 E019 0.7770552 0.0152866832 7.162491e-02 1.460443e-01 4 83484824 83484985 162 - 0.127 0.457 2.454
ENSG00000163322 E020 26.7220429 0.0077309494 1.907767e-06 1.490641e-05 4 83484986 83485149 164 - 1.256 1.630 1.291
ENSG00000163322 E021 0.0000000       4 83485290 83485555 266 -      
ENSG00000163322 E022 0.0000000       4 83523096 83523348 253 -