ENSG00000163293

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000295461 ENSG00000163293 HEK293_OSMI2_2hA HEK293_TMG_2hB NIPAL1 protein_coding protein_coding 1.701535 0.4999167 3.147305 0.06588766 0.1358919 2.63036 1.16177292 0.22976850 2.0897702 0.03366471 0.05800186 3.130517 0.61321667 0.49390000 0.6679333 0.17403333 0.46003860 0.03631697 FALSE TRUE
ENST00000500571 ENSG00000163293 HEK293_OSMI2_2hA HEK293_TMG_2hB NIPAL1 protein_coding processed_transcript 1.701535 0.4999167 3.147305 0.06588766 0.1358919 2.63036 0.09320783 0.00000000 0.1898912 0.00000000 0.18989117 4.321143 0.09278333 0.00000000 0.0556000 0.05560000 1.00000000 0.03631697   FALSE
ENST00000508180 ENSG00000163293 HEK293_OSMI2_2hA HEK293_TMG_2hB NIPAL1 protein_coding processed_transcript 1.701535 0.4999167 3.147305 0.06588766 0.1358919 2.63036 0.13750404 0.00000000 0.4008416 0.00000000 0.12703563 5.360510 0.05288333 0.00000000 0.1245000 0.12450000 0.04540251 0.03631697 FALSE TRUE
ENST00000511123 ENSG00000163293 HEK293_OSMI2_2hA HEK293_TMG_2hB NIPAL1 protein_coding protein_coding 1.701535 0.4999167 3.147305 0.06588766 0.1358919 2.63036 0.04271760 0.01105856 0.0000000 0.01105856 0.00000000 -1.074407 0.06057083 0.01783333 0.0000000 -0.01783333 0.33314511 0.03631697   FALSE
ENST00000513724 ENSG00000163293 HEK293_OSMI2_2hA HEK293_TMG_2hB NIPAL1 protein_coding processed_transcript 1.701535 0.4999167 3.147305 0.06588766 0.1358919 2.63036 0.03869048 0.22332947 0.0000000 0.12142059 0.00000000 -4.544297 0.07653750 0.39710000 0.0000000 -0.39710000 0.03631697 0.03631697   FALSE
MSTRG.24837.1 ENSG00000163293 HEK293_OSMI2_2hA HEK293_TMG_2hB NIPAL1 protein_coding   1.701535 0.4999167 3.147305 0.06588766 0.1358919 2.63036 0.11626932 0.03576015 0.2243598 0.03576015 0.11244424 2.356561 0.05493333 0.09113333 0.0745000 -0.01663333 0.98207940 0.03631697 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000163293 E001 1.0319116 0.0134466006 2.042958e-03 7.359568e-03 4 47914142 47914226 85 + 0.095 0.652 3.842
ENSG00000163293 E002 6.0055915 0.0030389172 4.141369e-07 3.723635e-06 4 47914227 47914704 478 + 0.535 1.199 2.607
ENSG00000163293 E003 0.0000000       4 47959024 47959141 118 +      
ENSG00000163293 E004 0.6256415 0.0178719024 4.637576e-01 6.035251e-01 4 48016772 48016778 7 + 0.135 0.272 1.257
ENSG00000163293 E005 5.0971739 0.0032532991 1.542918e-01 2.674890e-01 4 48016779 48016809 31 + 0.619 0.851 0.949
ENSG00000163293 E006 7.1028118 0.0024562504 7.433423e-02 1.504799e-01 4 48016810 48016851 42 + 0.730 0.987 0.994
ENSG00000163293 E007 6.3341877 0.0026585307 8.593593e-02 1.688811e-01 4 48016852 48016885 34 + 0.689 0.947 1.011
ENSG00000163293 E008 10.3660649 0.0021248937 2.045251e-01 3.318204e-01 4 48025068 48025126 59 + 0.892 1.059 0.619
ENSG00000163293 E009 12.4783442 0.0015619006 9.611844e-01 9.795714e-01 4 48025127 48025334 208 + 0.989 0.987 -0.006
ENSG00000163293 E010 6.6839595 0.0027362017 4.531161e-01 5.938820e-01 4 48030120 48030176 57 + 0.777 0.652 -0.516
ENSG00000163293 E011 7.2511857 0.0023564778 1.418296e-01 2.505426e-01 4 48032993 48033083 91 + 0.819 0.558 -1.097
ENSG00000163293 E012 12.2491473 0.0057460556 5.916259e-02 1.251974e-01 4 48034881 48035041 161 + 1.015 0.729 -1.104
ENSG00000163293 E013 65.3730291 0.0004293918 1.016897e-04 5.296894e-04 4 48035562 48037278 1717 + 1.699 1.461 -0.813
ENSG00000163293 E014 8.0210669 0.0021964523 8.112155e-01 8.802056e-01 4 48037279 48037341 63 + 0.827 0.794 -0.128
ENSG00000163293 E015 41.6805280 0.0005337135 2.930843e-01 4.341166e-01 4 48037342 48038700 1359 + 1.492 1.419 -0.249
ENSG00000163293 E016 44.6654059 0.0006363068 2.087314e-01 3.369835e-01 4 48038701 48040173 1473 + 1.498 1.585 0.296