ENSG00000163214

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000452978 ENSG00000163214 HEK293_OSMI2_2hA HEK293_TMG_2hB DHX57 protein_coding protein_coding 16.42326 10.64535 22.78421 1.008971 0.1096978 1.097089 1.5549665 3.121809 0.2362565 0.6975441 0.1501504 -3.6687623 0.13315417 0.2878000 0.01043333 -0.2773667 0.0002563908 0.0001985972 FALSE TRUE
ENST00000457308 ENSG00000163214 HEK293_OSMI2_2hA HEK293_TMG_2hB DHX57 protein_coding protein_coding 16.42326 10.64535 22.78421 1.008971 0.1096978 1.097089 12.8238821 5.916573 20.2366065 0.5954725 0.5604689 1.7724101 0.72466667 0.5664000 0.88800000 0.3216000 0.0003122026 0.0001985972 FALSE TRUE
ENST00000497514 ENSG00000163214 HEK293_OSMI2_2hA HEK293_TMG_2hB DHX57 protein_coding retained_intron 16.42326 10.64535 22.78421 1.008971 0.1096978 1.097089 0.7841468 1.301215 0.8858894 0.3633398 0.1482842 -0.5495114 0.05816667 0.1174333 0.03893333 -0.0785000 0.0158095585 0.0001985972 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000163214 E001 0.3299976 0.0274424043 5.870957e-02   2 38797729 38797735 7 - 0.000 0.292 12.560
ENSG00000163214 E002 85.1077622 0.0003618545 7.446040e-17 3.255550e-15 2 38797736 38797940 205 - 1.785 2.092 1.034
ENSG00000163214 E003 59.6174846 0.0013236145 4.602103e-11 8.593935e-10 2 38797941 38797967 27 - 1.633 1.936 1.021
ENSG00000163214 E004 213.0110450 0.0010136921 1.194723e-20 8.263482e-19 2 38797968 38798279 312 - 2.210 2.456 0.818
ENSG00000163214 E005 142.7190650 0.0002783509 1.484036e-14 4.772469e-13 2 38798280 38798351 72 - 2.051 2.268 0.727
ENSG00000163214 E006 145.6951594 0.0003404633 7.825234e-12 1.657870e-10 2 38798352 38798442 91 - 2.072 2.264 0.642
ENSG00000163214 E007 201.5501359 0.0003094132 1.098374e-12 2.674941e-11 2 38802715 38802915 201 - 2.220 2.390 0.566
ENSG00000163214 E008 2.0817956 0.0610170227 3.118809e-01 4.544084e-01 2 38802916 38803023 108 - 0.406 0.587 0.885
ENSG00000163214 E009 2.5904683 0.0058285789 2.200433e-01 3.504631e-01 2 38806404 38806558 155 - 0.612 0.387 -1.106
ENSG00000163214 E010 170.0025221 0.0002640750 1.390639e-09 2.007868e-08 2 38806559 38806693 135 - 2.152 2.307 0.517
ENSG00000163214 E011 121.8601041 0.0002878462 7.203308e-09 9.111343e-08 2 38813821 38813895 75 - 2.001 2.176 0.587
ENSG00000163214 E012 152.0874175 0.0002385857 1.055625e-05 6.978575e-05 2 38815521 38815655 135 - 2.124 2.239 0.386
ENSG00000163214 E013 1.2157955 0.0101979050 7.174283e-01 8.128039e-01 2 38815875 38816185 311 - 0.367 0.292 -0.469
ENSG00000163214 E014 0.2924217 0.0290785164 5.295539e-01   2 38818873 38818876 4 - 0.159 0.000 -10.628
ENSG00000163214 E015 104.7038709 0.0002720228 1.261785e-01 2.288303e-01 2 38818877 38818960 84 - 1.990 2.031 0.136
ENSG00000163214 E016 108.1530766 0.0028511090 3.881397e-02 8.883991e-02 2 38819049 38819144 96 - 1.993 2.066 0.245
ENSG00000163214 E017 176.6500697 0.0002053376 1.354779e-02 3.719207e-02 2 38822993 38823269 277 - 2.213 2.267 0.180
ENSG00000163214 E018 129.7752526 0.0026127683 2.641010e-01 4.020438e-01 2 38825847 38826047 201 - 2.089 2.119 0.102
ENSG00000163214 E019 115.8864647 0.0004773310 2.541819e-01 3.908255e-01 2 38826516 38826689 174 - 2.070 2.015 -0.184
ENSG00000163214 E020 82.0275524 0.0010080889 6.115078e-02 1.285681e-01 2 38828340 38828436 97 - 1.937 1.842 -0.322
ENSG00000163214 E021 87.9404068 0.0003847341 2.428506e-03 8.547891e-03 2 38837831 38837947 117 - 1.978 1.839 -0.469
ENSG00000163214 E022 132.7981847 0.0002390321 4.239204e-04 1.866123e-03 2 38843005 38843210 206 - 2.152 2.019 -0.444
ENSG00000163214 E023 74.9187262 0.0003210546 1.882186e-02 4.894102e-02 2 38847019 38847073 55 - 1.903 1.785 -0.398
ENSG00000163214 E024 117.1282191 0.0021912790 1.913542e-04 9.268632e-04 2 38848269 38848402 134 - 2.107 1.934 -0.580
ENSG00000163214 E025 5.3874055 0.0039872690 1.553456e-01 2.688606e-01 2 38853045 38854053 1009 - 0.857 0.639 -0.883
ENSG00000163214 E026 117.7159100 0.0114174393 3.083394e-02 7.359375e-02 2 38854054 38854178 125 - 2.104 1.952 -0.510
ENSG00000163214 E027 2.8713504 0.0127456704 4.055600e-02 9.203988e-02 2 38854179 38855056 878 - 0.677 0.292 -1.970
ENSG00000163214 E028 144.2875287 0.0016617281 3.203648e-05 1.892608e-04 2 38855057 38855252 196 - 2.198 2.027 -0.570
ENSG00000163214 E029 0.8148214 0.0238265047 5.986066e-01 7.201550e-01 2 38855253 38855275 23 - 0.275 0.170 -0.881
ENSG00000163214 E030 51.3215800 0.0004655787 7.967055e-03 2.369405e-02 2 38856340 38856362 23 - 1.752 1.595 -0.533
ENSG00000163214 E031 86.0478202 0.0003057069 5.447068e-05 3.040904e-04 2 38856363 38856461 99 - 1.979 1.795 -0.622
ENSG00000163214 E032 0.2214452 0.0421404811 2.149160e-01   2 38856663 38856794 132 - 0.000 0.170 11.591
ENSG00000163214 E033 0.2214452 0.0421404811 2.149160e-01   2 38856795 38857144 350 - 0.000 0.170 11.591
ENSG00000163214 E034 100.9428340 0.0003226470 7.203581e-09 9.111343e-08 2 38858661 38858836 176 - 2.063 1.818 -0.826
ENSG00000163214 E035 288.8403047 0.0066080763 9.837504e-09 1.214919e-07 2 38860999 38861785 787 - 2.523 2.250 -0.909
ENSG00000163214 E036 53.9827856 0.0036613321 5.117773e-07 4.517925e-06 2 38861786 38861837 52 - 1.813 1.481 -1.130
ENSG00000163214 E037 118.1543570 0.0002827351 4.090152e-18 2.090280e-16 2 38862145 38862333 189 - 2.153 1.805 -1.169
ENSG00000163214 E038 100.6006460 0.0003027715 6.851970e-19 3.850742e-17 2 38863361 38863519 159 - 2.092 1.702 -1.313
ENSG00000163214 E039 0.0000000       2 38867109 38867238 130 -      
ENSG00000163214 E040 96.9186706 0.0044367063 2.748821e-08 3.117925e-07 2 38868182 38868411 230 - 2.058 1.747 -1.046
ENSG00000163214 E041 2.9936058 0.0057364926 1.366827e-01 2.434552e-01 2 38875567 38875786 220 - 0.657 0.387 -1.297
ENSG00000163214 E042 52.6032492 0.0008089712 2.641561e-03 9.195467e-03 2 38875787 38875934 148 - 1.764 1.584 -0.608