ENSG00000163053

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000295190 ENSG00000163053 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC16A14 protein_coding protein_coding 2.900073 2.059077 3.72428 0.2768298 0.2354348 0.8518431 2.0032256 0.6809897 3.233782 0.07539656 0.1059819 2.230941 0.6142125 0.3496333 0.87543333 0.5258 0.0007244700 0.0003510182 FALSE TRUE
ENST00000457406 ENSG00000163053 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC16A14 protein_coding protein_coding 2.900073 2.059077 3.72428 0.2768298 0.2354348 0.8518431 0.8112243 1.3321459 0.394647 0.29576836 0.2255092 -1.729806 0.3573833 0.6308333 0.09973333 -0.5311 0.0003510182 0.0003510182 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000163053 E001 0.3289534 0.0297332656 5.600838e-01   2 230034794 230034981 188 - 0.093 0.159 0.878
ENSG00000163053 E002 130.9481668 0.0243621877 1.071516e-05 7.072519e-05 2 230034982 230037531 2550 - 2.216 1.881 -1.124
ENSG00000163053 E003 0.4815130 0.0202958126 2.114424e-01 3.401909e-01 2 230045657 230045669 13 - 0.093 0.274 1.875
ENSG00000163053 E004 3.0549618 0.0095030970 4.727384e-04 2.055847e-03 2 230045670 230045744 75 - 0.343 0.828 2.255
ENSG00000163053 E005 79.9510954 0.0003701076 6.729554e-07 5.797870e-06 2 230045745 230046614 870 - 1.862 1.957 0.318
ENSG00000163053 E006 18.8017877 0.0182527062 1.333482e-02 3.670101e-02 2 230046615 230046722 108 - 1.220 1.378 0.551
ENSG00000163053 E007 15.7463124 0.0167132196 7.453770e-01 8.334235e-01 2 230049761 230049904 144 - 1.238 1.190 -0.170
ENSG00000163053 E008 19.7933894 0.0096429304 6.170044e-01 7.349043e-01 2 230059094 230059226 133 - 1.359 1.237 -0.425
ENSG00000163053 E009 13.5510352 0.0015748595 2.027348e-01 3.296471e-01 2 230059227 230059298 72 - 1.227 1.029 -0.708
ENSG00000163053 E010 13.9069488 0.0019877575 2.609707e-01 3.985660e-01 2 230059299 230059366 68 - 1.152 1.179 0.096
ENSG00000163053 E011 0.0000000       2 230067099 230067237 139 -      
ENSG00000163053 E012 0.0000000       2 230068032 230068485 454 -      
ENSG00000163053 E013 0.0000000       2 230068486 230068554 69 -      
ENSG00000163053 E014 31.1060345 0.0158104856 3.912167e-03 1.289705e-02 2 230068555 230068993 439 - 1.437 1.583 0.498