ENSG00000162994

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000401408 ENSG00000162994 HEK293_OSMI2_2hA HEK293_TMG_2hB CLHC1 protein_coding protein_coding 2.966097 1.689037 4.006598 0.1606394 0.6962423 1.241257 0.29622164 0.14340539 0.4752604 0.06032701 0.06614464 1.6614099 0.09754583 0.08696667 0.126466667 0.03950000 7.761951e-01 4.736099e-06 FALSE TRUE
ENST00000407122 ENSG00000162994 HEK293_OSMI2_2hA HEK293_TMG_2hB CLHC1 protein_coding protein_coding 2.966097 1.689037 4.006598 0.1606394 0.6962423 1.241257 0.16724997 0.00000000 0.2924010 0.00000000 0.15414854 4.9183910 0.04649583 0.00000000 0.064933333 0.06493333 1.514356e-02 4.736099e-06 FALSE TRUE
ENST00000425841 ENSG00000162994 HEK293_OSMI2_2hA HEK293_TMG_2hB CLHC1 protein_coding protein_coding 2.966097 1.689037 4.006598 0.1606394 0.6962423 1.241257 0.21842587 0.21582396 0.4217692 0.21582396 0.24346300 0.9350617 0.07644583 0.14260000 0.110066667 -0.03253333 8.219281e-01 4.736099e-06 TRUE TRUE
ENST00000463300 ENSG00000162994 HEK293_OSMI2_2hA HEK293_TMG_2hB CLHC1 protein_coding processed_transcript 2.966097 1.689037 4.006598 0.1606394 0.6962423 1.241257 0.21970175 0.07598156 0.4683870 0.04672857 0.20680424 2.4760788 0.06625417 0.04950000 0.107866667 0.05836667 4.977496e-01 4.736099e-06   FALSE
ENST00000464243 ENSG00000162994 HEK293_OSMI2_2hA HEK293_TMG_2hB CLHC1 protein_coding processed_transcript 2.966097 1.689037 4.006598 0.1606394 0.6962423 1.241257 0.34701270 0.00000000 0.7176186 0.00000000 0.03239792 6.1851105 0.10272917 0.00000000 0.191100000 0.19110000 4.736099e-06 4.736099e-06   FALSE
MSTRG.18419.14 ENSG00000162994 HEK293_OSMI2_2hA HEK293_TMG_2hB CLHC1 protein_coding   2.966097 1.689037 4.006598 0.1606394 0.6962423 1.241257 0.06145926 0.19773646 0.0201213 0.16751017 0.02012130 -2.7858987 0.02737500 0.10150000 0.003733333 -0.09776667 3.069307e-01 4.736099e-06 TRUE TRUE
MSTRG.18419.16 ENSG00000162994 HEK293_OSMI2_2hA HEK293_TMG_2hB CLHC1 protein_coding   2.966097 1.689037 4.006598 0.1606394 0.6962423 1.241257 0.61748364 0.68900784 0.2995368 0.14178280 0.17490201 -1.1751976 0.25696250 0.40750000 0.079733333 -0.32776667 9.095471e-02 4.736099e-06 TRUE TRUE
MSTRG.18419.6 ENSG00000162994 HEK293_OSMI2_2hA HEK293_TMG_2hB CLHC1 protein_coding   2.966097 1.689037 4.006598 0.1606394 0.6962423 1.241257 0.24711769 0.13332751 0.3764987 0.13332751 0.21777731 1.4311480 0.07254167 0.06633333 0.098300000 0.03196667 7.994082e-01 4.736099e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000162994 E001 0.0000000       2 55151167 55151202 36 -      
ENSG00000162994 E002 0.3666179 0.030886289 5.559077e-01 6.846909e-01 2 55170190 55170270 81 - 0.087 0.170 1.105
ENSG00000162994 E003 18.4780545 0.020411226 2.528031e-01 3.892299e-01 2 55172547 55174790 2244 - 1.317 1.195 -0.432
ENSG00000162994 E004 5.3885529 0.019887422 2.284078e-02 5.741092e-02 2 55174791 55175638 848 - 0.897 0.528 -1.535
ENSG00000162994 E005 0.7374030 0.015374776 5.904532e-01 7.133175e-01 2 55175639 55175648 10 - 0.276 0.169 -0.900
ENSG00000162994 E006 1.0329452 0.012974807 8.864858e-01 9.314012e-01 2 55175649 55175661 13 - 0.324 0.290 -0.223
ENSG00000162994 E007 1.4768824 0.009562849 4.358392e-01 5.779957e-01 2 55175662 55175668 7 - 0.444 0.291 -0.900
ENSG00000162994 E008 3.7620410 0.052806783 7.526176e-01 8.387571e-01 2 55175669 55175746 78 - 0.699 0.633 -0.279
ENSG00000162994 E009 9.1062440 0.001843608 5.750229e-01 7.007432e-01 2 55175747 55175860 114 - 1.014 0.936 -0.291
ENSG00000162994 E010 10.3294264 0.001740954 7.022304e-01 8.010854e-01 2 55175861 55175986 126 - 1.059 1.002 -0.206
ENSG00000162994 E011 7.6541517 0.002398836 5.762473e-01 7.017328e-01 2 55177602 55177743 142 - 0.909 0.959 0.188
ENSG00000162994 E012 4.6959065 0.014608128 9.756561e-02 1.867663e-01 2 55177744 55177781 38 - 0.658 0.883 0.902
ENSG00000162994 E013 9.5834775 0.001781515 8.046394e-01 8.756945e-01 2 55180510 55180712 203 - 1.023 0.981 -0.155
ENSG00000162994 E014 6.6816531 0.004868303 2.241479e-02 5.654096e-02 2 55181570 55181744 175 - 0.965 0.637 -1.301
ENSG00000162994 E015 1.2501371 0.027800799 6.922298e-01 7.935235e-01 2 55205712 55205728 17 - 0.324 0.385 0.363
ENSG00000162994 E016 5.6385898 0.003800318 5.199778e-02 1.127193e-01 2 55206270 55206376 107 - 0.885 0.587 -1.220
ENSG00000162994 E017 2.2270580 0.007152875 1.520546e-01 2.644816e-01 2 55206818 55206940 123 - 0.564 0.291 -1.482
ENSG00000162994 E018 1.3424206 0.010190309 1.990406e-02 5.128617e-02 2 55208626 55208710 85 - 0.477 0.000 -12.447
ENSG00000162994 E019 2.3732749 0.006438736 3.434854e-01 4.875239e-01 2 55209404 55209516 113 - 0.564 0.386 -0.898
ENSG00000162994 E020 7.4271496 0.003646415 2.460314e-02 6.106049e-02 2 55209630 55209831 202 - 0.995 0.683 -1.221
ENSG00000162994 E021 5.0214956 0.004364427 1.160034e-02 3.258474e-02 2 55212673 55212806 134 - 0.872 0.463 -1.757
ENSG00000162994 E022 3.5122343 0.078155424 7.369987e-03 2.217915e-02 2 55217811 55217933 123 - 0.771 0.170 -3.356
ENSG00000162994 E023 1.6411850 0.008823774 8.725746e-03 2.559996e-02 2 55217934 55217998 65 - 0.537 0.000 -12.638
ENSG00000162994 E024 3.2611517 0.004722156 1.062525e-04 5.507308e-04 2 55218120 55218344 225 - 0.770 0.000 -13.247
ENSG00000162994 E025 4.8111992 0.003615506 5.760721e-04 2.444674e-03 2 55218345 55218600 256 - 0.885 0.291 -2.805
ENSG00000162994 E026 1.6681517 0.008397694 3.417063e-01 4.856210e-01 2 55222235 55222271 37 - 0.477 0.291 -1.069
ENSG00000162994 E027 4.2109406 0.023963103 1.872725e-02 4.873784e-02 2 55222272 55222359 88 - 0.817 0.385 -1.963
ENSG00000162994 E028 5.0495977 0.003417891 2.901248e-04 1.339799e-03 2 55222360 55222493 134 - 0.909 0.291 -2.899
ENSG00000162994 E029 8.8018968 0.002161663 4.743632e-04 2.062203e-03 2 55223996 55224190 195 - 0.817 1.171 1.315
ENSG00000162994 E030 33.2166400 0.002105647 2.553261e-24 2.614636e-22 2 55224191 55225313 1123 - 1.213 1.786 1.972
ENSG00000162994 E031 4.6639320 0.003950717 6.915606e-01 7.930107e-01 2 55228032 55228204 173 - 0.753 0.683 -0.290
ENSG00000162994 E032 2.7451888 0.088954164 6.681434e-01 7.752096e-01 2 55232004 55232222 219 - 0.509 0.594 0.396
ENSG00000162994 E033 3.9694880 0.533413686 7.695992e-01 8.512033e-01 2 55232223 55232235 13 - 0.567 0.809 1.017
ENSG00000162994 E034 6.5807216 0.053653749 6.106469e-01 7.299536e-01 2 55232236 55232354 119 - 0.831 0.891 0.231
ENSG00000162994 E035 0.0000000       2 55232492 55232563 72 -