ENSG00000162976

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000295083 ENSG00000162976 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC66A3 protein_coding protein_coding 3.178538 2.824576 3.985352 0.3395191 0.3037034 0.4951898 1.7917595 1.5540352 2.8432268 0.36974985 0.05600272 0.8673215 0.53081667 0.5369667 0.71993333 0.1829667 2.090862e-01 9.222438e-06 FALSE TRUE
ENST00000441908 ENSG00000162976 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC66A3 protein_coding protein_coding 3.178538 2.824576 3.985352 0.3395191 0.3037034 0.4951898 0.4896499 0.0000000 0.5624930 0.00000000 0.16269808 5.8391861 0.13889583 0.0000000 0.13980000 0.1398000 9.222438e-06 9.222438e-06 FALSE TRUE
ENST00000445921 ENSG00000162976 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC66A3 protein_coding nonsense_mediated_decay 3.178538 2.824576 3.985352 0.3395191 0.3037034 0.4951898 0.3625631 0.7403750 0.2439757 0.09168603 0.08265760 -1.5629212 0.13157917 0.2774000 0.06176667 -0.2156333 6.296369e-03 9.222438e-06 TRUE TRUE
ENST00000464700 ENSG00000162976 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC66A3 protein_coding retained_intron 3.178538 2.824576 3.985352 0.3395191 0.3037034 0.4951898 0.2283717 0.4112813 0.0000000 0.21303014 0.00000000 -5.3967119 0.09111667 0.1360333 0.00000000 -0.1360333 7.711065e-02 9.222438e-06 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000162976 E001 0.0000000       2 11155198 11155266 69 +      
ENSG00000162976 E002 0.1451727 0.0423404542 6.733928e-01   2 11155372 11155430 59 + 0.105 0.000 -10.158
ENSG00000162976 E003 4.3460612 0.0038002472 3.781788e-01 5.224707e-01 2 11155431 11155466 36 + 0.658 0.782 0.511
ENSG00000162976 E004 6.8485971 0.0173294938 1.817400e-01 3.034103e-01 2 11155467 11155514 48 + 0.791 0.965 0.666
ENSG00000162976 E005 6.7034244 0.0177485319 1.420535e-01 2.508400e-01 2 11155515 11155526 12 + 0.771 0.965 0.744
ENSG00000162976 E006 5.7811991 0.0499724204 7.554482e-01 8.407740e-01 2 11155527 11155532 6 + 0.791 0.842 0.201
ENSG00000162976 E007 4.9059997 0.0488256947 9.799295e-01 9.914746e-01 2 11155533 11155534 2 + 0.751 0.759 0.033
ENSG00000162976 E008 9.5538586 0.0019608833 4.280325e-01 5.708396e-01 2 11155535 11155608 74 + 0.976 1.060 0.311
ENSG00000162976 E009 12.8895758 0.0015298988 1.231916e-01 2.245274e-01 2 11155609 11155689 81 + 1.066 1.208 0.511
ENSG00000162976 E010 0.1472490 0.0439122521 6.719995e-01   2 11160290 11160465 176 + 0.105 0.000 -10.158
ENSG00000162976 E011 20.8399992 0.0011515844 3.569879e-01 5.014033e-01 2 11160466 11160548 83 + 1.309 1.378 0.242
ENSG00000162976 E012 25.4239649 0.0008677554 1.861400e-01 3.089122e-01 2 11160625 11160694 70 + 1.379 1.468 0.307
ENSG00000162976 E013 1.7962813 0.0082807263 1.422069e-01 2.510500e-01 2 11160695 11160748 54 + 0.320 0.570 1.317
ENSG00000162976 E014 0.6599951 0.0192785134 1.600000e-02 4.273390e-02 2 11160749 11160819 71 + 0.000 0.407 12.901
ENSG00000162976 E015 20.9447771 0.0009487143 8.326189e-01 8.951251e-01 2 11164204 11164261 58 + 1.331 1.349 0.063
ENSG00000162976 E016 23.3378510 0.0008545132 1.434531e-01 2.527115e-01 2 11171925 11172011 87 + 1.425 1.326 -0.344
ENSG00000162976 E017 16.6832755 0.0011722913 7.321863e-02 1.486699e-01 2 11172012 11172045 34 + 1.309 1.164 -0.510
ENSG00000162976 E018 0.1472490 0.0439122521 6.719995e-01   2 11172046 11172046 1 + 0.105 0.000 -10.158
ENSG00000162976 E019 6.3530224 0.0512530538 6.151075e-06 4.291617e-05 2 11172751 11172824 74 + 0.374 1.117 3.149
ENSG00000162976 E020 14.5531355 0.0015510875 1.015221e-02 2.913835e-02 2 11174968 11175009 42 + 1.285 1.060 -0.802
ENSG00000162976 E021 22.9316380 0.0100362632 3.308592e-02 7.798092e-02 2 11177737 11177904 168 + 1.455 1.282 -0.602
ENSG00000162976 E022 18.3847225 0.0010952156 1.526690e-01 2.653019e-01 2 11177905 11178033 129 + 1.337 1.228 -0.381
ENSG00000162976 E023 60.9371392 0.0210075360 5.155249e-01 6.501874e-01 2 11178034 11178870 837 + 1.809 1.778 -0.106