ENSG00000162971

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000354611 ENSG00000162971 HEK293_OSMI2_2hA HEK293_TMG_2hB TYW5 protein_coding protein_coding 3.011939 0.9280829 4.443614 0.06491927 0.0367981 2.247189 0.8674140 0.37632337 1.6332623 0.07830404 0.05484109 2.088682 0.3377333 0.39896667 0.36743333 -0.03153333 0.79150112 0.00391586 FALSE TRUE
ENST00000441832 ENSG00000162971 HEK293_OSMI2_2hA HEK293_TMG_2hB TYW5 protein_coding nonsense_mediated_decay 3.011939 0.9280829 4.443614 0.06491927 0.0367981 2.247189 1.2683300 0.44402585 1.4187011 0.11088290 0.12442092 1.653858 0.4439417 0.47483333 0.31893333 -0.15590000 0.43603694 0.00391586 TRUE FALSE
ENST00000452512 ENSG00000162971 HEK293_OSMI2_2hA HEK293_TMG_2hB TYW5 protein_coding processed_transcript 3.011939 0.9280829 4.443614 0.06491927 0.0367981 2.247189 0.4465134 0.07958526 0.7857227 0.07958526 0.13104705 3.150932 0.1100375 0.09466667 0.17720000 0.08253333 0.47091908 0.00391586 FALSE TRUE
ENST00000483328 ENSG00000162971 HEK293_OSMI2_2hA HEK293_TMG_2hB TYW5 protein_coding nonsense_mediated_decay 3.011939 0.9280829 4.443614 0.06491927 0.0367981 2.247189 0.3213664 0.00000000 0.4133587 0.00000000 0.03776082 5.403809 0.0725875 0.00000000 0.09306667 0.09306667 0.00391586 0.00391586 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000162971 E001 26.139233 0.0008835095 0.041955392 0.094644302 2 199928913 199929974 1062 - 1.321 1.480 0.551
ENSG00000162971 E002 30.440753 0.0007644482 0.052389884 0.113416485 2 199929975 199931332 1358 - 1.446 1.302 -0.502
ENSG00000162971 E003 24.405374 0.0012318330 0.199283082 0.325341314 2 199931333 199931898 566 - 1.343 1.239 -0.366
ENSG00000162971 E004 101.066977 0.0066800648 0.075409158 0.152175020 2 199931899 199933323 1425 - 1.908 1.988 0.268
ENSG00000162971 E005 26.172697 0.0025388403 0.203691748 0.330819665 2 199935931 199936047 117 - 1.331 1.439 0.376
ENSG00000162971 E006 23.820944 0.0008884511 0.868690502 0.919615226 2 199936405 199936492 88 - 1.321 1.316 -0.017
ENSG00000162971 E007 25.235247 0.0011292186 0.333681905 0.477422501 2 199938933 199939070 138 - 1.358 1.287 -0.250
ENSG00000162971 E008 15.503787 0.0025353986 0.358967898 0.503299990 2 199940089 199940133 45 - 1.163 1.074 -0.319
ENSG00000162971 E009 5.207993 0.0031680591 0.260834730 0.398409704 2 199942058 199943764 1707 - 0.754 0.571 -0.781
ENSG00000162971 E010 15.663454 0.0011180904 0.892847683 0.935518262 2 199943765 199943834 70 - 1.149 1.143 -0.019
ENSG00000162971 E011 10.444796 0.0020903351 0.001522119 0.005708535 2 199943835 199948317 4483 - 1.046 0.571 -1.896
ENSG00000162971 E012 4.879062 0.0036476373 0.349724950 0.493917703 2 199948318 199948336 19 - 0.661 0.809 0.602
ENSG00000162971 E013 27.599672 0.0008021709 0.890628192 0.934153688 2 199948337 199948472 136 - 1.376 1.394 0.065
ENSG00000162971 E014 21.666937 0.0016485903 0.849480769 0.906607559 2 199955393 199955736 344 - 1.280 1.271 -0.032