ENSG00000162819

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000340934 ENSG00000162819 HEK293_OSMI2_2hA HEK293_TMG_2hB BROX protein_coding protein_coding 13.06682 10.68766 16.91688 0.2619795 0.7601438 0.6620212 3.9093054 1.1706444 6.650124 0.5249108 0.6071529 2.495975 0.25563750 0.11200000 0.39536667 0.28336667 1.046216e-01 3.506653e-41 FALSE TRUE
ENST00000426638 ENSG00000162819 HEK293_OSMI2_2hA HEK293_TMG_2hB BROX protein_coding protein_coding 13.06682 10.68766 16.91688 0.2619795 0.7601438 0.6620212 0.9168492 0.0000000 1.691830 0.0000000 0.2820317 7.410943 0.05127083 0.00000000 0.09923333 0.09923333 7.255178e-14 3.506653e-41 FALSE FALSE
ENST00000496267 ENSG00000162819 HEK293_OSMI2_2hA HEK293_TMG_2hB BROX protein_coding processed_transcript 13.06682 10.68766 16.91688 0.2619795 0.7601438 0.6620212 4.3540453 7.5158733 2.812394 0.2373542 0.1116250 -1.414940 0.41602083 0.70356667 0.16666667 -0.53690000 3.506653e-41 3.506653e-41   FALSE
MSTRG.3103.9 ENSG00000162819 HEK293_OSMI2_2hA HEK293_TMG_2hB BROX protein_coding   13.06682 10.68766 16.91688 0.2619795 0.7601438 0.6620212 2.1607687 0.8841549 3.796316 0.8841549 0.6689309 2.089799 0.15104583 0.07903333 0.22370000 0.14466667 1.640146e-01 3.506653e-41 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000162819 E001 1.5706715 0.1650079127 4.953023e-01 6.321310e-01 1 222712553 222712558 6 + 0.342 0.493 0.818
ENSG00000162819 E002 1.8349806 0.0346322386 8.282423e-01 8.920628e-01 1 222712559 222712563 5 + 0.447 0.413 -0.183
ENSG00000162819 E003 4.5942628 0.0609938549 1.971345e-01 3.226455e-01 1 222712564 222712574 11 + 0.643 0.865 0.896
ENSG00000162819 E004 12.0056808 0.0019350146 1.187657e-05 7.756952e-05 1 222712575 222712582 8 + 0.903 1.335 1.559
ENSG00000162819 E005 106.9323116 0.0006536278 8.319381e-34 1.865186e-31 1 222712583 222712942 360 + 1.814 2.247 1.452
ENSG00000162819 E006 0.8200183 0.0166037724 5.712811e-01 6.975983e-01 1 222713321 222713354 34 + 0.204 0.314 0.818
ENSG00000162819 E007 1.1187827 0.0409108359 9.788548e-01 9.908412e-01 1 222713355 222713368 14 + 0.301 0.314 0.081
ENSG00000162819 E008 0.8189741 0.0329471637 6.926182e-01 7.938091e-01 1 222713369 222713375 7 + 0.255 0.185 -0.594
ENSG00000162819 E009 1.4874769 0.1019785433 7.511535e-01 8.376772e-01 1 222713376 222713380 5 + 0.343 0.415 0.412
ENSG00000162819 E010 2.5226002 0.0230623297 4.087635e-01 5.523822e-01 1 222713381 222713400 20 + 0.557 0.413 -0.710
ENSG00000162819 E011 5.9878325 0.0486538645 6.076580e-02 1.279171e-01 1 222713401 222713416 16 + 0.893 0.562 -1.364
ENSG00000162819 E012 59.4077412 0.0005047382 1.053502e-67 1.435848e-64 1 222713588 222713667 80 + 1.301 2.135 2.833
ENSG00000162819 E013 42.2304751 0.0010505014 1.880323e-53 1.386076e-50 1 222713668 222713706 39 + 1.107 2.004 3.082
ENSG00000162819 E014 46.7810305 0.0021280337 7.113196e-53 5.104445e-50 1 222713707 222713985 279 + 1.165 2.047 3.020
ENSG00000162819 E015 3.5159158 0.0045429647 4.041138e-01 5.478221e-01 1 222715145 222715269 125 + 0.580 0.719 0.596
ENSG00000162819 E016 41.6034254 0.0005919181 3.581746e-14 1.085161e-12 1 222715684 222715800 117 + 1.706 1.169 -1.856
ENSG00000162819 E017 0.4418608 0.0249314093 3.590979e-01 5.034363e-01 1 222717858 222718094 237 + 0.204 0.000 -9.537
ENSG00000162819 E018 44.9015756 0.0074451073 6.918709e-08 7.248403e-07 1 222718925 222719031 107 + 1.719 1.305 -1.424
ENSG00000162819 E019 43.6202399 0.0005450414 1.311774e-10 2.271172e-09 1 222719263 222719359 97 + 1.709 1.291 -1.439
ENSG00000162819 E020 48.3643633 0.0012459313 1.916609e-11 3.810031e-10 1 222722419 222722514 96 + 1.755 1.325 -1.472
ENSG00000162819 E021 23.7929864 0.0008204203 2.066792e-06 1.602732e-05 1 222724092 222724099 8 + 1.454 1.044 -1.447
ENSG00000162819 E022 42.1034274 0.0005406108 9.601483e-10 1.428437e-08 1 222724100 222724164 65 + 1.692 1.291 -1.380
ENSG00000162819 E023 55.5038892 0.0005361439 7.303725e-16 2.809480e-14 1 222725450 222725555 106 + 1.821 1.335 -1.660
ENSG00000162819 E024 41.0709860 0.0018174943 1.890911e-09 2.660236e-08 1 222727168 222727257 90 + 1.683 1.267 -1.434
ENSG00000162819 E025 45.5236796 0.0004772214 2.348985e-07 2.221820e-06 1 222728743 222728828 86 + 1.713 1.404 -1.057
ENSG00000162819 E026 47.3636454 0.0005955669 1.239018e-09 1.803243e-08 1 222729620 222729701 82 + 1.737 1.366 -1.271
ENSG00000162819 E027 64.0542377 0.0050110746 8.520615e-08 8.766331e-07 1 222730027 222730177 151 + 1.860 1.536 -1.099
ENSG00000162819 E028 65.4557816 0.0004222002 4.662339e-11 8.701272e-10 1 222731357 222731516 160 + 1.871 1.536 -1.135
ENSG00000162819 E029 501.4109411 0.0029649764 5.917001e-01 7.144183e-01 1 222732628 222735196 2569 + 2.668 2.671 0.010