ENSG00000162772

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000341491 ENSG00000162772 HEK293_OSMI2_2hA HEK293_TMG_2hB ATF3 protein_coding protein_coding 17.90694 23.76162 7.535556 1.984963 0.228133 -1.655541 13.313175 15.318361 6.8608377 0.33849246 0.21539061 -1.157646 0.76782917 0.65183333 0.91040000 0.2585667 1.234861e-07 1.3806e-12 FALSE TRUE
ENST00000465155 ENSG00000162772 HEK293_OSMI2_2hA HEK293_TMG_2hB ATF3 protein_coding retained_intron 17.90694 23.76162 7.535556 1.984963 0.228133 -1.655541 1.039396 1.269988 0.2826338 0.05907419 0.04078197 -2.128962 0.05622083 0.05466667 0.03756667 -0.0171000 5.386318e-01 1.3806e-12 FALSE FALSE
ENST00000613104 ENSG00000162772 HEK293_OSMI2_2hA HEK293_TMG_2hB ATF3 protein_coding protein_coding 17.90694 23.76162 7.535556 1.984963 0.228133 -1.655541 1.884027 5.192430 0.0000000 1.51110071 0.00000000 -9.023042 0.08657083 0.21093333 0.00000000 -0.2109333 1.380600e-12 1.3806e-12 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000162772 E001 0.1451727 0.0429294069 1.448724e-01   1 212565334 212565483 150 + 0.198 0.000 -10.653
ENSG00000162772 E002 0.1451727 0.0429294069 1.448724e-01   1 212608628 212608669 42 + 0.198 0.000 -12.572
ENSG00000162772 E003 0.9630129 0.0143129149 5.956030e-01 7.176625e-01 1 212608670 212608760 91 + 0.334 0.238 -0.661
ENSG00000162772 E004 50.0521582 0.0007302745 4.936823e-02 1.080333e-01 1 212608761 212608914 154 + 1.700 1.596 -0.354
ENSG00000162772 E005 36.9842446 0.0006066208 1.525000e-01 2.650812e-01 1 212608915 212608930 16 + 1.560 1.473 -0.295
ENSG00000162772 E006 3.7307236 0.0047499124 5.348115e-01 6.668055e-01 1 212609069 212609271 203 + 0.520 0.633 0.510
ENSG00000162772 E007 3.9784359 0.2841267142 3.507968e-01 4.949718e-01 1 212613436 212614127 692 + 0.795 0.562 -0.984
ENSG00000162772 E008 30.9304812 0.0007223580 9.977675e-01 1.000000e+00 1 212615018 212615021 4 + 1.413 1.419 0.023
ENSG00000162772 E009 30.7821880 0.0007482479 9.701026e-01 9.852997e-01 1 212615022 212615022 1 + 1.413 1.416 0.013
ENSG00000162772 E010 43.1242317 0.0005555306 1.007122e-01 1.915491e-01 1 212615023 212615045 23 + 1.630 1.538 -0.313
ENSG00000162772 E011 45.0822728 0.0005019079 1.969717e-01 3.224347e-01 1 212615046 212615060 15 + 1.636 1.567 -0.235
ENSG00000162772 E012 39.6157475 0.0005845396 1.792323e-01 3.001599e-01 1 212615061 212615061 1 + 1.586 1.509 -0.264
ENSG00000162772 E013 80.7011664 0.0003402910 2.967417e-01 4.380031e-01 1 212615062 212615147 86 + 1.858 1.818 -0.134
ENSG00000162772 E014 44.2810355 0.0005198673 7.726566e-01 8.532643e-01 1 212615148 212615151 4 + 1.553 1.577 0.085
ENSG00000162772 E015 62.6504086 0.0004404970 4.892585e-01 6.267089e-01 1 212615152 212615177 26 + 1.743 1.715 -0.096
ENSG00000162772 E016 98.8140985 0.0003592109 2.273126e-02 5.719632e-02 1 212615178 212615261 84 + 1.979 1.895 -0.281
ENSG00000162772 E017 133.9001314 0.0002957500 1.099180e-02 3.114341e-02 1 212618127 212618234 108 + 2.108 2.029 -0.264
ENSG00000162772 E018 27.3908896 0.0134483604 2.514586e-02 6.218097e-02 1 212618235 212619045 811 + 1.173 1.400 0.798
ENSG00000162772 E019 9.9509287 0.0023167762 7.439936e-04 3.057360e-03 1 212619046 212619266 221 + 0.520 1.036 2.094
ENSG00000162772 E020 9.0443431 0.0019174287 9.285775e-05 4.884398e-04 1 212619267 212619357 91 + 0.334 0.999 2.956
ENSG00000162772 E021 74.7404287 0.0075184594 2.521758e-01 3.884978e-01 1 212619358 212619366 9 + 1.844 1.784 -0.201
ENSG00000162772 E022 633.9482338 0.0005566155 3.371685e-06 2.496407e-05 1 212619367 212620777 1411 + 2.658 2.732 0.246