ENSG00000162733

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000367921 ENSG00000162733 HEK293_OSMI2_2hA HEK293_TMG_2hB DDR2 protein_coding protein_coding 1.47392 0.6847993 2.365562 0.06137555 0.1225322 1.773601 0.8979870 0.41171920 1.6686881 0.20828326 0.07330006 1.992980 0.6202125 0.6383667 0.7061667 0.0678000 0.87860613 0.01067483 FALSE TRUE
ENST00000446985 ENSG00000162733 HEK293_OSMI2_2hA HEK293_TMG_2hB DDR2 protein_coding protein_coding 1.47392 0.6847993 2.365562 0.06137555 0.1225322 1.773601 0.1546595 0.18501215 0.0000000 0.18501215 0.00000000 -4.285492 0.1436208 0.2444667 0.0000000 -0.2444667 0.62467406 0.01067483 FALSE TRUE
ENST00000672207 ENSG00000162733 HEK293_OSMI2_2hA HEK293_TMG_2hB DDR2 protein_coding processed_transcript 1.47392 0.6847993 2.365562 0.06137555 0.1225322 1.773601 0.2099432 0.02080623 0.4594245 0.02080623 0.03262941 3.929599 0.1185500 0.0283000 0.1939333 0.1656333 0.01067483 0.01067483 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000162733 E001 0.1472490 0.0440886209 1.000000e+00   1 162631373 162631447 75 + 0.075 0.000 -8.199
ENSG00000162733 E002 0.0000000       1 162631946 162632010 65 +      
ENSG00000162733 E003 0.0000000       1 162632011 162632093 83 +      
ENSG00000162733 E004 0.9578041 0.0136968632 6.088715e-01 7.284701e-01 1 162632437 162632445 9 + 0.287 0.195 -0.727
ENSG00000162733 E005 1.3974748 0.0138830830 2.863956e-01 4.267786e-01 1 162632446 162632463 18 + 0.398 0.195 -1.405
ENSG00000162733 E006 1.3974748 0.0138830830 2.863956e-01 4.267786e-01 1 162632464 162632464 1 + 0.398 0.195 -1.405
ENSG00000162733 E007 5.4649246 0.0034154241 4.435907e-01 5.851706e-01 1 162632465 162632631 167 + 0.796 0.695 -0.406
ENSG00000162733 E008 6.6763400 0.0050579043 4.852743e-01 6.231740e-01 1 162655211 162655374 164 + 0.867 0.785 -0.324
ENSG00000162733 E009 0.1451727 0.0429370340 1.000000e+00   1 162677234 162677346 113 + 0.075 0.000 -10.181
ENSG00000162733 E010 0.4804688 0.0213958278 7.541469e-01 8.398537e-01 1 162705052 162705131 80 + 0.138 0.195 0.593
ENSG00000162733 E011 6.6574532 0.0159238330 2.821781e-01 4.221525e-01 1 162719037 162719145 109 + 0.889 0.741 -0.578
ENSG00000162733 E012 8.0830406 0.0288049253 3.379910e-01 4.818774e-01 1 162753095 162753197 103 + 0.956 0.823 -0.510
ENSG00000162733 E013 13.5824588 0.0095677902 5.779117e-01 7.030846e-01 1 162754624 162754855 232 + 1.138 1.090 -0.172
ENSG00000162733 E014 11.5730732 0.0033711554 5.666280e-01 6.935749e-01 1 162755156 162755303 148 + 1.074 1.026 -0.178
ENSG00000162733 E015 8.6169810 0.0022247128 1.716143e-01 2.903100e-01 1 162755664 162755769 106 + 0.983 0.823 -0.608
ENSG00000162733 E016 10.8424462 0.0016507544 2.222366e-03 7.912731e-03 1 162759796 162759979 184 + 1.114 0.742 -1.407
ENSG00000162733 E017 17.4208942 0.0011364717 1.138412e-02 3.208300e-02 1 162761211 162761454 244 + 1.278 1.048 -0.822
ENSG00000162733 E018 11.8467077 0.0407901048 1.402186e-01 2.483220e-01 1 162766001 162766063 63 + 1.114 0.923 -0.700
ENSG00000162733 E019 7.3911706 0.0023413067 2.606644e-02 6.408265e-02 1 162767229 162767236 8 + 0.948 0.643 -1.214
ENSG00000162733 E020 10.0591710 0.0020197896 2.153437e-02 5.471450e-02 1 162767237 162767289 53 + 1.060 0.785 -1.044
ENSG00000162733 E021 12.0799294 0.0015649463 9.339280e-01 9.623703e-01 1 162767290 162767359 70 + 1.067 1.090 0.082
ENSG00000162733 E022 16.3823437 0.0012904783 4.822098e-01 6.203741e-01 1 162770302 162770512 211 + 1.214 1.163 -0.180
ENSG00000162733 E023 0.9159869 0.0138699391 6.090260e-01 7.285933e-01 1 162770513 162770651 139 + 0.287 0.195 -0.728
ENSG00000162733 E024 1.4070518 0.0093773203 8.183928e-01 8.852096e-01 1 162770652 162770894 243 + 0.364 0.328 -0.214
ENSG00000162733 E025 15.4003996 0.0014825993 9.458721e-01 9.700087e-01 1 162772024 162772247 224 + 1.172 1.180 0.027
ENSG00000162733 E026 0.0000000       1 162772248 162772435 188 +      
ENSG00000162733 E027 13.7433331 0.0019385257 3.998256e-01 5.435454e-01 1 162773469 162773596 128 + 1.101 1.196 0.339
ENSG00000162733 E028 17.9144722 0.0010650085 8.133583e-02 1.616452e-01 1 162775652 162775843 192 + 1.274 1.128 -0.518
ENSG00000162733 E029 21.5826691 0.0009178571 2.695785e-01 4.080771e-01 1 162776136 162776370 235 + 1.331 1.254 -0.268
ENSG00000162733 E030 14.7484549 0.0013111719 8.558148e-01 9.108309e-01 1 162778580 162778729 150 + 1.150 1.146 -0.012
ENSG00000162733 E031 197.1376176 0.0019511350 1.838092e-09 2.592859e-08 1 162780112 162787405 7294 + 2.202 2.354 0.509