ENSG00000162650

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000369869 ENSG00000162650 HEK293_OSMI2_2hA HEK293_TMG_2hB ATXN7L2 protein_coding retained_intron 9.32906 13.20887 7.066855 0.9232845 0.1873419 -0.9014184 0.5929875 0.8354454 0.0000000 0.55394317 0.00000000 -6.4016396 0.05766250 0.06033333 0.0000000 -0.06033333 2.370808e-01 8.752152e-05 FALSE TRUE
ENST00000497545 ENSG00000162650 HEK293_OSMI2_2hA HEK293_TMG_2hB ATXN7L2 protein_coding retained_intron 9.32906 13.20887 7.066855 0.9232845 0.1873419 -0.9014184 1.0479890 0.6831587 1.0633431 0.16760937 0.02835365 0.6308538 0.12745417 0.05056667 0.1508000 0.10023333 8.752152e-05 8.752152e-05 TRUE TRUE
ENST00000683729 ENSG00000162650 HEK293_OSMI2_2hA HEK293_TMG_2hB ATXN7L2 protein_coding protein_coding 9.32906 13.20887 7.066855 0.9232845 0.1873419 -0.9014184 5.9316667 9.9803695 4.7551405 0.75505178 0.12205143 -1.0680193 0.61604583 0.76203333 0.6743333 -0.08770000 5.816396e-01 8.752152e-05 FALSE TRUE
ENST00000684472 ENSG00000162650 HEK293_OSMI2_2hA HEK293_TMG_2hB ATXN7L2 protein_coding nonsense_mediated_decay 9.32906 13.20887 7.066855 0.9232845 0.1873419 -0.9014184 0.7826135 0.4139168 0.7635725 0.07713282 0.14258576 0.8677553 0.09142083 0.03126667 0.1071667 0.07590000 6.708059e-04 8.752152e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000162650 E001 2.945352 0.0053608802 9.749815e-01 9.884195e-01 1 109483479 109483904 426 + 0.567 0.557 -0.044
ENSG00000162650 E002 1.653373 0.0110766674 9.199783e-01 9.534515e-01 1 109483905 109483938 34 + 0.370 0.384 0.084
ENSG00000162650 E003 2.933798 0.1157095385 8.031468e-01 8.746931e-01 1 109483939 109483953 15 + 0.510 0.568 0.272
ENSG00000162650 E004 38.867334 0.0057324850 7.911648e-02 1.581036e-01 1 109483954 109484080 127 + 1.639 1.518 -0.414
ENSG00000162650 E005 8.388328 0.0019750392 2.454853e-03 8.628764e-03 1 109484651 109484831 181 + 1.146 0.809 -1.255
ENSG00000162650 E006 8.432452 0.0070364779 7.354366e-05 3.971672e-04 1 109485332 109485509 178 + 1.198 0.741 -1.715
ENSG00000162650 E007 5.025174 0.0209709965 2.748254e-02 6.697392e-02 1 109485510 109485518 9 + 0.935 0.612 -1.299
ENSG00000162650 E008 18.703009 0.0296132732 3.065844e-04 1.406161e-03 1 109485546 109486056 511 + 1.485 1.085 -1.404
ENSG00000162650 E009 50.407271 0.0087087839 8.145224e-03 2.415075e-02 1 109486057 109486122 66 + 1.782 1.606 -0.596
ENSG00000162650 E010 8.707065 0.0078267957 4.307622e-06 3.109652e-05 1 109486123 109486304 182 + 1.233 0.702 -1.996
ENSG00000162650 E011 5.702751 0.0030966650 9.360729e-04 3.740481e-03 1 109486305 109486505 201 + 1.035 0.610 -1.678
ENSG00000162650 E012 43.547152 0.0043503812 3.508918e-01 4.950802e-01 1 109486506 109486610 105 + 1.652 1.589 -0.216
ENSG00000162650 E013 80.745277 0.0046328065 8.603383e-01 9.138996e-01 1 109487007 109487217 211 + 1.887 1.873 -0.046
ENSG00000162650 E014 66.803128 0.0006853580 9.642922e-01 9.814968e-01 1 109487518 109487613 96 + 1.801 1.793 -0.028
ENSG00000162650 E015 106.497628 0.0010521824 3.885811e-01 5.326584e-01 1 109487614 109487804 191 + 2.021 1.983 -0.127
ENSG00000162650 E016 73.479583 0.0008812819 5.664082e-02 1.208328e-01 1 109488383 109488465 83 + 1.779 1.859 0.269
ENSG00000162650 E017 117.995567 0.0002568120 2.025733e-03 7.306684e-03 1 109488847 109489100 254 + 1.963 2.064 0.337
ENSG00000162650 E018 12.329353 0.0014362950 3.539299e-05 2.066795e-04 1 109489101 109489282 182 + 1.316 0.921 -1.424
ENSG00000162650 E019 19.128999 0.0030861094 4.111597e-06 2.981207e-05 1 109489283 109489550 268 + 1.485 1.109 -1.318
ENSG00000162650 E020 10.895219 0.0021142773 5.799004e-04 2.459543e-03 1 109489551 109489706 156 + 1.245 0.896 -1.271
ENSG00000162650 E021 11.819116 0.0412692469 4.145089e-02 9.372185e-02 1 109489707 109489929 223 + 1.233 0.965 -0.969
ENSG00000162650 E022 102.567480 0.0003607478 2.648884e-01 4.028864e-01 1 109489930 109490128 199 + 1.950 1.985 0.117
ENSG00000162650 E023 85.531527 0.0003962245 3.157041e-01 4.584809e-01 1 109490271 109490392 122 + 1.872 1.906 0.116
ENSG00000162650 E024 332.643677 0.0004746561 4.365470e-08 4.764518e-07 1 109490922 109491717 796 + 2.409 2.514 0.349
ENSG00000162650 E025 7.594211 0.0081858997 2.091478e-01 3.375080e-01 1 109491718 109492026 309 + 0.773 0.934 0.625
ENSG00000162650 E026 5.870065 0.0118782930 5.907897e-01 7.136284e-01 1 109492027 109492104 78 + 0.739 0.811 0.288
ENSG00000162650 E027 46.713108 0.0012915638 7.380006e-04 3.035827e-03 1 109492586 109492804 219 + 1.497 1.691 0.659