ENSG00000162614

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000330010 ENSG00000162614 HEK293_OSMI2_2hA HEK293_TMG_2hB NEXN protein_coding protein_coding 2.215141 1.145134 4.081909 0.1877254 0.3634936 1.824714 1.2587736 0.93461880 2.5045544 0.02345580 0.2365313 1.412499 0.62298750 0.85146667 0.6251 -0.2263667 0.38161605 0.01168074 FALSE TRUE
ENST00000401035 ENSG00000162614 HEK293_OSMI2_2hA HEK293_TMG_2hB NEXN protein_coding protein_coding 2.215141 1.145134 4.081909 0.1877254 0.3634936 1.824714 0.4247727 0.03024101 0.9406703 0.03024101 0.5737569 4.562207 0.13513750 0.02926667 0.2112 0.1819333 0.60067174 0.01168074 FALSE TRUE
ENST00000440324 ENSG00000162614 HEK293_OSMI2_2hA HEK293_TMG_2hB NEXN protein_coding protein_coding 2.215141 1.145134 4.081909 0.1877254 0.3634936 1.824714 0.2765038 0.00000000 0.5944139 0.00000000 0.1866913 5.917465 0.09341667 0.00000000 0.1549 0.1549000 0.01168074 0.01168074 FALSE TRUE
ENST00000470735 ENSG00000162614 HEK293_OSMI2_2hA HEK293_TMG_2hB NEXN protein_coding retained_intron 2.215141 1.145134 4.081909 0.1877254 0.3634936 1.824714 0.1530901 0.11245111 0.0000000 0.11245111 0.0000000 -3.614134 0.08841667 0.07440000 0.0000 -0.0744000 0.49480213 0.01168074 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000162614 E001 1.0987209 0.0174273899 3.488551e-02 0.0814331945 1 77888513 77888514 2 + 0.139 0.512 2.574
ENSG00000162614 E002 4.5613963 0.1367335396 6.854296e-01 0.7881699465 1 77888515 77888623 109 + 0.695 0.742 0.191
ENSG00000162614 E003 6.0059116 0.0702004163 6.740030e-01 0.7795475778 1 77888624 77888661 38 + 0.798 0.852 0.210
ENSG00000162614 E004 11.7998318 0.0772434005 6.436871e-01 0.7561657998 1 77888662 77888759 98 + 1.097 1.013 -0.306
ENSG00000162614 E005 15.5189734 0.0486998266 3.695978e-01 0.5139965880 1 77916055 77916133 79 + 1.216 1.097 -0.425
ENSG00000162614 E006 2.1571283 0.0302225520 8.955957e-02 0.1744513111 1 77917566 77917757 192 + 0.539 0.194 -2.129
ENSG00000162614 E007 16.9699938 0.0011937035 6.847870e-02 0.1408510349 1 77917960 77918038 79 + 1.260 1.087 -0.616
ENSG00000162614 E008 10.1203660 0.0017668802 2.914040e-01 0.4322731833 1 77918125 77918127 3 + 1.042 0.918 -0.458
ENSG00000162614 E009 25.7758015 0.0007689176 4.094802e-02 0.0927839298 1 77918128 77918273 146 + 1.423 1.265 -0.552
ENSG00000162614 E010 12.4904818 0.0164902212 4.484944e-02 0.0999221173 1 77925188 77925229 42 + 1.141 0.891 -0.922
ENSG00000162614 E011 17.8899931 0.0127168723 6.700231e-02 0.1383869471 1 77926414 77926568 155 + 1.282 1.088 -0.688
ENSG00000162614 E012 9.2519473 0.0113389327 2.061034e-01 0.3338142438 1 77926569 77926611 43 + 1.019 0.855 -0.617
ENSG00000162614 E013 0.1817044 0.0404976402 1.556356e-01   1 77926612 77926715 104 + 0.000 0.193 11.846
ENSG00000162614 E014 19.6098907 0.0009305639 2.228217e-01 0.3537403429 1 77926716 77926892 177 + 1.299 1.193 -0.373
ENSG00000162614 E015 20.4712467 0.0009635407 3.005510e-01 0.4422577993 1 77929316 77929504 189 + 1.311 1.223 -0.309
ENSG00000162614 E016 0.1472490 0.0432266390 1.000000e+00   1 77931662 77932054 393 + 0.075 0.000 -8.980
ENSG00000162614 E017 0.0000000       1 77933048 77933281 234 +      
ENSG00000162614 E018 21.4551246 0.0132775203 4.347378e-01 0.5770481998 1 77933282 77933479 198 + 1.327 1.253 -0.257
ENSG00000162614 E019 20.1186404 0.0010526275 1.142070e-01 0.2116743474 1 77935823 77936044 222 + 1.246 1.370 0.432
ENSG00000162614 E020 0.4428904 0.3313826806 6.822323e-02 0.1404216167 1 77941711 77942022 312 + 0.000 0.336 12.904
ENSG00000162614 E021 22.8093718 0.0013616969 5.005567e-01 0.6367907645 1 77942023 77942208 186 + 1.319 1.370 0.178
ENSG00000162614 E022 28.2278254 0.0016897121 3.143080e-03 0.0106862234 1 77942461 77942818 358 + 1.356 1.554 0.679
ENSG00000162614 E023 5.1095016 0.0080263922 4.587487e-02 0.1018069932 1 77942819 77942899 81 + 0.643 0.919 1.104
ENSG00000162614 E024 38.6616538 0.0029356425 4.218831e-05 0.0002418599 1 77942900 77943895 996 + 1.467 1.708 0.820