ENSG00000162522

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000294521 ENSG00000162522 HEK293_OSMI2_2hA HEK293_TMG_2hB KIAA1522 protein_coding protein_coding 18.36602 29.07291 13.21312 1.601756 0.03953296 -1.137109 5.924586 13.305076 2.570165 0.5321446 0.2390680 -2.3675256 0.28070833 0.46240000 0.1946333 -0.26776667 4.557476e-05 4.557476e-05 FALSE TRUE
ENST00000373481 ENSG00000162522 HEK293_OSMI2_2hA HEK293_TMG_2hB KIAA1522 protein_coding protein_coding 18.36602 29.07291 13.21312 1.601756 0.03953296 -1.137109 6.867627 8.348287 6.047083 0.8831387 0.6769262 -0.4645843 0.40464583 0.28660000 0.4573333 0.17073333 4.533750e-02 4.557476e-05 FALSE TRUE
ENST00000401073 ENSG00000162522 HEK293_OSMI2_2hA HEK293_TMG_2hB KIAA1522 protein_coding protein_coding 18.36602 29.07291 13.21312 1.601756 0.03953296 -1.137109 1.894318 2.048116 1.822771 0.5652782 0.1471915 -0.1672982 0.10810833 0.06916667 0.1379000 0.06873333 8.356324e-02 4.557476e-05 FALSE TRUE
ENST00000468130 ENSG00000162522 HEK293_OSMI2_2hA HEK293_TMG_2hB KIAA1522 protein_coding retained_intron 18.36602 29.07291 13.21312 1.601756 0.03953296 -1.137109 1.515304 2.801940 1.444822 0.3076295 0.1205691 -0.9507237 0.08149167 0.09576667 0.1093000 0.01353333 7.128180e-01 4.557476e-05 FALSE TRUE
MSTRG.778.3 ENSG00000162522 HEK293_OSMI2_2hA HEK293_TMG_2hB KIAA1522 protein_coding   18.36602 29.07291 13.21312 1.601756 0.03953296 -1.137109 1.934225 2.210087 1.328281 0.7190607 0.7160795 -0.7302359 0.11140833 0.07453333 0.1008333 0.02630000 9.749119e-01 4.557476e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000162522 E001 2.495052 0.1223026116 3.056195e-03 0.0104318219 1 32741830 32741888 59 + 0.805 0.249 -2.798
ENSG00000162522 E002 17.329350 0.0223443160 3.778282e-02 0.0869324211 1 32741889 32742189 301 + 1.321 1.182 -0.488
ENSG00000162522 E003 7.399046 0.0341708990 7.066847e-01 0.8048000203 1 32754011 32754018 8 + 0.883 0.901 0.067
ENSG00000162522 E004 10.938806 0.0072543952 1.879420e-01 0.3111299447 1 32754019 32754020 2 + 1.087 1.020 -0.242
ENSG00000162522 E005 43.712434 0.0052605298 8.252794e-02 0.1634964111 1 32754021 32754180 160 + 1.637 1.609 -0.096
ENSG00000162522 E006 0.000000       1 32765634 32765666 33 +      
ENSG00000162522 E007 0.000000       1 32765667 32765827 161 +      
ENSG00000162522 E008 126.119046 0.0162031301 1.077292e-02 0.0306215774 1 32767787 32767957 171 + 2.130 2.047 -0.277
ENSG00000162522 E009 98.808232 0.0059324284 2.514704e-04 0.0011813501 1 32768062 32768117 56 + 2.034 1.933 -0.338
ENSG00000162522 E010 127.770837 0.0076866097 3.231922e-03 0.0109497630 1 32768652 32768775 124 + 2.121 2.058 -0.211
ENSG00000162522 E011 22.204591 0.0009026638 7.424823e-02 0.1503270674 1 32768776 32768946 171 + 1.179 1.402 0.781
ENSG00000162522 E012 76.358455 0.0082474176 3.876809e-03 0.0127972991 1 32769673 32769786 114 + 1.911 1.829 -0.278
ENSG00000162522 E013 110.096973 0.0071577184 1.644403e-04 0.0008111211 1 32769880 32770110 231 + 2.086 1.979 -0.359
ENSG00000162522 E014 71.394888 0.0138623546 1.112963e-01 0.2074555181 1 32770111 32770220 110 + 1.849 1.825 -0.080
ENSG00000162522 E015 662.355029 0.0009107592 5.963994e-05 0.0003296650 1 32770221 32772441 2221 + 2.781 2.799 0.060
ENSG00000162522 E016 1381.240514 0.0109906052 8.203661e-06 0.0000555831 1 32772865 32774970 2106 + 2.963 3.177 0.713