ENSG00000162413

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000377658 ENSG00000162413 HEK293_OSMI2_2hA HEK293_TMG_2hB KLHL21 protein_coding protein_coding 34.3803 49.49962 30.25383 3.626675 1.105022 -0.7101144 27.648480 44.984239 22.548355 3.323661 0.103253 -0.9960784 0.7802375 0.9087 0.7471000 -0.16160000 6.263355e-09 1.427446e-29 FALSE TRUE
ENST00000377663 ENSG00000162413 HEK293_OSMI2_2hA HEK293_TMG_2hB KLHL21 protein_coding protein_coding 34.3803 49.49962 30.25383 3.626675 1.105022 -0.7101144 4.448445 4.455763 3.335202 0.309670 0.284083 -0.4168132 0.1395833 0.0901 0.1100333 0.01993333 3.419765e-01 1.427446e-29 FALSE TRUE
ENST00000496707 ENSG00000162413 HEK293_OSMI2_2hA HEK293_TMG_2hB KLHL21 protein_coding protein_coding 34.3803 49.49962 30.25383 3.626675 1.105022 -0.7101144 2.196918 0.000000 4.258366 0.000000 0.741408 8.7375401 0.0776875 0.0000 0.1393333 0.13933333 1.427446e-29 1.427446e-29 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000162413 E001 3578.5388783 0.0028118108 1.174778e-14 3.823365e-13 1 6590724 6593192 2469 - 3.402 3.570 0.558
ENSG00000162413 E002 377.9649622 0.0003183981 1.481616e-01 2.591789e-01 1 6593193 6593380 188 - 2.520 2.556 0.118
ENSG00000162413 E003 566.2412128 0.0006361297 7.764424e-03 2.318643e-02 1 6593381 6593658 278 - 2.708 2.724 0.054
ENSG00000162413 E004 73.8613744 0.0004048119 4.239642e-04 1.866185e-03 1 6593659 6594014 356 - 1.898 1.810 -0.295
ENSG00000162413 E005 36.7766005 0.0005581337 1.515370e-01 2.637605e-01 1 6594015 6594080 66 - 1.561 1.537 -0.083
ENSG00000162413 E006 29.6610917 0.0006800268 1.579685e-01 2.724296e-01 1 6594081 6594110 30 - 1.476 1.444 -0.111
ENSG00000162413 E007 170.2287381 0.0003846259 1.663945e-05 1.050730e-04 1 6594111 6595484 1374 - 2.242 2.180 -0.205
ENSG00000162413 E008 268.4758779 0.0002779342 4.749428e-05 2.690604e-04 1 6595485 6595557 73 - 2.417 2.386 -0.103
ENSG00000162413 E009 617.2562180 0.0004455068 1.328348e-11 2.711762e-10 1 6599047 6599452 406 - 2.790 2.742 -0.162
ENSG00000162413 E010 0.6245948 0.0366529134 2.620245e-01 3.997467e-01 1 6599750 6599816 67 - 0.000 0.241 10.280
ENSG00000162413 E011 468.2232084 0.0069855240 1.917928e-12 4.487412e-11 1 6601797 6603001 1205 - 2.775 2.558 -0.724
ENSG00000162413 E012 0.2934659 0.0301719000 6.035477e-01   1 6614450 6614607 158 - 0.156 0.095 -0.815