ENSG00000162377

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000371538 ENSG00000162377 HEK293_OSMI2_2hA HEK293_TMG_2hB COA7 protein_coding protein_coding 24.73065 19.06445 33.13534 2.08975 1.623836 0.7971649 18.6631045 11.715308 28.0906636 0.4026412 1.1263128 1.260978338 0.72704167 0.62650000 0.84913333 0.22263333 0.009216485 0.009216485 FALSE TRUE
ENST00000486918 ENSG00000162377 HEK293_OSMI2_2hA HEK293_TMG_2hB COA7 protein_coding processed_transcript 24.73065 19.06445 33.13534 2.08975 1.623836 0.7971649 1.3690437 1.223951 1.2217066 0.5243668 0.4504885 -0.002626977 0.05797083 0.06876667 0.03810000 -0.03066667 0.855968929 0.009216485 FALSE TRUE
MSTRG.1193.1 ENSG00000162377 HEK293_OSMI2_2hA HEK293_TMG_2hB COA7 protein_coding   24.73065 19.06445 33.13534 2.08975 1.623836 0.7971649 0.9977557 1.302283 0.5175072 0.3326088 0.2391399 -1.314816146 0.04990833 0.06626667 0.01593333 -0.05033333 0.021562693 0.009216485 FALSE FALSE
MSTRG.1193.4 ENSG00000162377 HEK293_OSMI2_2hA HEK293_TMG_2hB COA7 protein_coding   24.73065 19.06445 33.13534 2.08975 1.623836 0.7971649 3.7007502 4.822905 3.3054656 1.6302107 1.1703533 -0.543679235 0.16506667 0.23846667 0.09690000 -0.14156667 0.237720085 0.009216485 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000162377 E001 3.126942 0.005565415 0.0002773674 0.001287461 1 52663558 52663676 119 - 0.301 0.841 2.573
ENSG00000162377 E002 2.132622 0.006770568 0.0141944231 0.038665251 1 52663677 52663758 82 - 0.301 0.683 1.934
ENSG00000162377 E003 1456.363623 0.006149672 0.0074944036 0.022497461 1 52684449 52687561 3113 - 3.192 3.104 -0.291
ENSG00000162377 E004 583.035176 0.005090254 0.0160327958 0.042809334 1 52687562 52688168 607 - 2.746 2.783 0.125
ENSG00000162377 E005 265.730705 0.001715131 0.0449729239 0.100146759 1 52692727 52692867 141 - 2.414 2.437 0.075
ENSG00000162377 E006 7.070194 0.192742042 0.2342275560 0.367429350 1 52697909 52698165 257 - 0.760 1.038 1.061
ENSG00000162377 E007 4.570616 0.003740422 0.4102841322 0.553884017 1 52698166 52698220 55 - 0.698 0.785 0.351
ENSG00000162377 E008 162.879238 0.001525895 0.4471883646 0.588604916 1 52698221 52698359 139 - 2.214 2.205 -0.032