ENSG00000162300

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000294258 ENSG00000162300 HEK293_OSMI2_2hA HEK293_TMG_2hB ZFPL1 protein_coding protein_coding 37.37643 61.1422 23.75393 1.798661 0.6794548 -1.363631 25.198674 44.8155333 16.016919 1.7413607 0.3544220 -1.483824 0.65451250 0.73266667 0.67453333 -0.05813333 0.07012978 0.04574204 FALSE  
ENST00000525509 ENSG00000162300 HEK293_OSMI2_2hA HEK293_TMG_2hB ZFPL1 protein_coding protein_coding 37.37643 61.1422 23.75393 1.798661 0.6794548 -1.363631 6.106595 10.1924778 2.495134 1.0639739 0.2442134 -2.025960 0.15746667 0.16673333 0.10463333 -0.06210000 0.04574204 0.04574204    
ENST00000533216 ENSG00000162300 HEK293_OSMI2_2hA HEK293_TMG_2hB ZFPL1 protein_coding retained_intron 37.37643 61.1422 23.75393 1.798661 0.6794548 -1.363631 1.720164 0.6305025 1.979798 0.4897833 0.4403444 1.635345 0.06015833 0.01013333 0.08456667 0.07443333 0.07908833 0.04574204 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000162300 E001 4.084643 0.1573566540 6.043840e-01 7.249645e-01 11 65084210 65084221 12 + 0.575 0.654 0.346
ENSG00000162300 E002 4.084643 0.1573566540 6.043840e-01 7.249645e-01 11 65084222 65084222 1 + 0.575 0.654 0.346
ENSG00000162300 E003 6.981732 0.2909299820 2.914227e-01 4.322976e-01 11 65084223 65084229 7 + 0.687 0.864 0.708
ENSG00000162300 E004 13.012236 0.0015249691 9.570974e-01 9.771092e-01 11 65084230 65084234 5 + 1.061 1.069 0.031
ENSG00000162300 E005 34.577170 0.0006052478 6.294685e-01 7.448341e-01 11 65084235 65084240 6 + 1.497 1.468 -0.102
ENSG00000162300 E006 35.683278 0.0006084484 6.949682e-01 7.956716e-01 11 65084241 65084241 1 + 1.505 1.482 -0.079
ENSG00000162300 E007 164.589363 0.0024967327 7.860109e-01 8.629562e-01 11 65084242 65084289 48 + 2.126 2.142 0.052
ENSG00000162300 E008 208.773611 0.0021899097 6.086681e-01 7.283327e-01 11 65084290 65084328 39 + 2.252 2.238 -0.044
ENSG00000162300 E009 206.647143 0.0033932339 5.227988e-01 6.565465e-01 11 65084329 65084360 32 + 2.252 2.232 -0.065
ENSG00000162300 E010 169.685318 0.0005641208 9.659523e-03 2.792607e-02 11 65084361 65084378 18 + 2.211 2.132 -0.263
ENSG00000162300 E011 5.338016 0.0030819712 6.735867e-02 1.389652e-01 11 65084379 65084518 140 + 0.913 0.666 -0.982
ENSG00000162300 E012 20.171215 0.0073062039 1.159782e-01 2.142057e-01 11 65084519 65084690 172 + 1.354 1.213 -0.493
ENSG00000162300 E013 280.231596 0.0007523529 9.500472e-01 9.727371e-01 11 65084691 65084782 92 + 2.365 2.367 0.007
ENSG00000162300 E014 178.251733 0.0002439683 8.501465e-02 1.674266e-01 11 65084783 65084800 18 + 2.124 2.182 0.193
ENSG00000162300 E015 33.226740 0.0006174783 3.727392e-03 1.237610e-02 11 65084801 65085012 212 + 1.272 1.498 0.783
ENSG00000162300 E016 186.881092 0.0008150197 2.636572e-01 4.015674e-01 11 65085115 65085152 38 + 2.157 2.198 0.135
ENSG00000162300 E017 215.370630 0.0016080189 4.023175e-01 5.460553e-01 11 65085153 65085226 74 + 2.269 2.246 -0.075
ENSG00000162300 E018 17.726580 0.0036722133 1.757592e-03 6.464318e-03 11 65085227 65085286 60 + 1.384 1.111 -0.964
ENSG00000162300 E019 13.781709 0.0029715871 3.996965e-03 1.313484e-02 11 65085287 65085314 28 + 1.284 1.009 -0.987
ENSG00000162300 E020 16.257331 0.0125287315 1.753960e-03 6.452715e-03 11 65085315 65085384 70 + 1.374 1.064 -1.097
ENSG00000162300 E021 82.187098 0.0015964642 1.459841e-20 9.973402e-19 11 65085385 65086338 954 + 2.112 1.713 -1.341
ENSG00000162300 E022 12.434737 0.0015708905 7.245066e-03 2.186156e-02 11 65086339 65086414 76 + 1.245 0.983 -0.945
ENSG00000162300 E023 322.376364 0.0013310293 9.208287e-01 9.540667e-01 11 65086415 65086608 194 + 2.426 2.429 0.008
ENSG00000162300 E024 5.493686 0.0032315878 7.997006e-03 2.377193e-02 11 65086706 65086719 14 + 0.993 0.648 -1.361
ENSG00000162300 E025 209.542712 0.0002091714 1.421428e-01 2.509532e-01 11 65086720 65086792 73 + 2.206 2.252 0.152
ENSG00000162300 E026 323.665243 0.0001770784 1.530902e-01 2.658805e-01 11 65086928 65087074 147 + 2.401 2.438 0.120
ENSG00000162300 E027 309.213153 0.0018072483 5.866578e-03 1.826228e-02 11 65087316 65087433 118 + 2.340 2.429 0.297
ENSG00000162300 E028 446.109125 0.0022119048 6.141608e-03 1.898222e-02 11 65087928 65088398 471 + 2.504 2.587 0.276