ENSG00000162222

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000316461 ENSG00000162222 HEK293_OSMI2_2hA HEK293_TMG_2hB TTC9C protein_coding protein_coding 35.97577 45.15581 34.2248 1.448344 0.3234737 -0.3997673 18.371491 23.743289 16.985909 1.8558237 0.8061164 -0.4829398 0.51074167 0.52426667 0.4967333 -0.02753333 0.81621361 0.01704791 FALSE  
ENST00000530625 ENSG00000162222 HEK293_OSMI2_2hA HEK293_TMG_2hB TTC9C protein_coding protein_coding 35.97577 45.15581 34.2248 1.448344 0.3234737 -0.3997673 2.134637 1.475263 3.329579 1.4752626 0.4378979 1.1689481 0.06146667 0.03486667 0.0971000 0.06223333 0.13732211 0.01704791 FALSE  
ENST00000601045 ENSG00000162222 HEK293_OSMI2_2hA HEK293_TMG_2hB TTC9C protein_coding retained_intron 35.97577 45.15581 34.2248 1.448344 0.3234737 -0.3997673 5.643621 9.232082 4.708791 0.2309046 0.6956549 -0.9698004 0.14994583 0.20500000 0.1374000 -0.06760000 0.14724262 0.01704791    
MSTRG.5617.9 ENSG00000162222 HEK293_OSMI2_2hA HEK293_TMG_2hB TTC9C protein_coding   35.97577 45.15581 34.2248 1.448344 0.3234737 -0.3997673 7.062013 6.801596 7.616505 0.4097455 0.4251580 0.1630293 0.20150000 0.15110000 0.2223667 0.07126667 0.01704791 0.01704791 TRUE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000162222 E001 4.0932797 0.0109895660 5.355635e-01 6.673654e-01 11 62727923 62728050 128 + 0.738 0.643 -0.394
ENSG00000162222 E002 0.7728880 0.0166932370 9.264564e-02 1.792591e-01 11 62728051 62728068 18 + 0.000 0.311 10.152
ENSG00000162222 E003 0.9244034 0.0148474572 3.630933e-01 5.075141e-01 11 62728069 62728102 34 + 0.148 0.311 1.363
ENSG00000162222 E004 1.9197833 0.0078128656 8.496140e-01 9.066723e-01 11 62728103 62728111 9 + 0.419 0.451 0.170
ENSG00000162222 E005 1.9197833 0.0078128656 8.496140e-01 9.066723e-01 11 62728112 62728114 3 + 0.419 0.451 0.170
ENSG00000162222 E006 8.3139504 0.0588311634 3.790668e-01 5.233540e-01 11 62728115 62728161 47 + 1.031 0.885 -0.546
ENSG00000162222 E007 26.3003512 0.0026265250 2.827968e-01 4.228392e-01 11 62728162 62728227 66 + 1.463 1.384 -0.270
ENSG00000162222 E008 7.3223700 0.0032334388 5.002688e-01 6.365398e-01 11 62728228 62728376 149 + 0.851 0.933 0.312
ENSG00000162222 E009 9.0238816 0.0019391011 2.267507e-01 3.585088e-01 11 62728377 62728485 109 + 1.063 0.933 -0.481
ENSG00000162222 E010 164.7239123 0.0003070812 1.750235e-02 4.605005e-02 11 62728486 62728641 156 + 2.246 2.180 -0.219
ENSG00000162222 E011 186.5051824 0.0002211307 1.114916e-02 3.152406e-02 11 62728642 62728736 95 + 2.297 2.232 -0.218
ENSG00000162222 E012 118.9285767 0.0002952651 4.419485e-01 5.836110e-01 11 62728737 62728822 86 + 2.045 2.071 0.086
ENSG00000162222 E013 397.0640533 0.0001536253 3.562123e-01 5.006071e-01 11 62728823 62729086 264 + 2.593 2.577 -0.053
ENSG00000162222 E014 8.7173095 0.0018966443 4.864532e-03 1.555315e-02 11 62733118 62733199 82 + 1.146 0.845 -1.116
ENSG00000162222 E015 213.5987000 0.0007721444 8.709877e-01 9.210495e-01 11 62735382 62735449 68 + 2.319 2.315 -0.013
ENSG00000162222 E016 279.5545565 0.0001928860 3.499056e-01 4.941008e-01 11 62735450 62735564 115 + 2.418 2.437 0.065
ENSG00000162222 E017 3.1879205 0.0178181501 1.499660e-02 4.048019e-02 11 62735565 62736243 679 + 0.825 0.450 -1.646
ENSG00000162222 E018 341.6312766 0.0001542178 3.185886e-02 7.558061e-02 11 62738288 62738490 203 + 2.492 2.532 0.135
ENSG00000162222 E019 173.1027320 0.0002441542 8.971545e-01 9.383299e-01 11 62738491 62738783 293 + 2.222 2.225 0.012
ENSG00000162222 E020 106.8406501 0.0002942553 1.322243e-01 2.372021e-01 11 62738784 62739075 292 + 2.049 1.997 -0.174
ENSG00000162222 E021 30.8091200 0.0008053291 1.075095e-05 7.092435e-05 11 62739948 62739975 28 + 1.266 1.574 1.063
ENSG00000162222 E022 70.0736160 0.0005433973 4.811850e-06 3.440044e-05 11 62739976 62740293 318 + 1.686 1.896 0.709